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A novel multiplex qPCR for the simultaneous detection of Salmonella spp., E. coli O157:H7 and Listeria monocytogenes from raw Atlantic Salmon fillets


A triplex qPCR assay was developed to screen for three major food-borne pathogens in fish. The assay is based on simultaneous qPCR amplification of specific target genomes using fluorophore labelled locked nucleic acid (LNA) TaqMan probes. The detection limit of the qPCR is 1 colony forming unit (cfu) per 1ml and the detection limit of the assay with 24h general enrichment is 1 cfu per 25g of fish meat.

Date Posted: 14/09/2009

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