Satellite Banner
Scientific Community
Become a Member | Sign in
Home>News>This Article

Improving Reproducibility in Real-Time PCR

Published: Tuesday, February 19, 2013
Last Updated: Tuesday, February 19, 2013
Bookmark and Share
Improved reproducibility and productivity by using VIAFLO 96 handheld electronic pipette.

INTEGRA has reported how a leading US clinical diagnostic laboratory has, using a VIAFLO 96 handheld electronic pipette, significantly improved the reproducibility and productivity with which it carries out Real-time PCR assays.

An important source of income for the clinical diagnostic lab is the screening of patient samples for the presence of disease markers by gene expression analysis.

This analysis involved transferring pre-treated patient samples from a 96-well plate source to a target PCR plate, adding the PCR master mix and then subsequently analyzing the plate by real-time PCR.

The lab traditionally processed around 100 plates per day and the work was all done using 12-channel pipettes.

Manually filling a 96-well plate, including changing of pipette tips after every sample transfer, was acknowledged to be a time consuming task and transferring the samples often proved to be prone to error.

Looking to improve this process the lab invested in an INTEGRA VIAFLO 96 handheld electronic pipette to both load the patient samples into the plates and then to transfer the PCR master mix to the reaction plate.

As a result - the work is now done in half the time. More important to the clinical diagnostic lab are two additional improvements.

Transfer mistakes from plate to plate have been eliminated and now the light-sensitive PCR master mix is only exposed to daylight for a few seconds prior to the reaction, as all plate wells can be processed at the same time.

According to the lab manager, the VIAFLO 96 has increased accuracy, robustness and precision of the assay in comparison to their previous use of manual 12 channel pipettes.

The INTEGRA VIAFLO 96 is a handheld 96-channel electronic pipette that enables fast, precise and easy simultaneous transfer of 96 samples.

Customers will immediately benefit from the increased productivity delivered by their VIAFLO 96. Fast replication or reformatting of 96 and 384 well plates and high precision transferring of reagents, compounds and solutions to or from microplates is now as easy as pipetting with a standard electronic pipette into a single tube.

Four pipetting heads with pipetting volumes up to 12.5 µl, 125 µl, 300 µl or 1250 µl are available for the VIAFLO 96. These pipetting heads are interchangeable within seconds enabling optimal matching of the available volume range to the application performed.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,600+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Scientific News
New Virus Identified In Blood Supply
Scientists have discovered a new virus that can be transmitted through the blood supply.
Tracking Changes in DNA Methylation In Real Time At Single-Cell Resolution
Whitehead Institute researchers have developed a methodology to monitor changes in DNA methylation over time in individual cells.
DNA Alterations as Among Earliest to Occur in Lung Cancer Development
Genetic footprints of precancer detectable in some blood samples.
Bar-Coding Technique Opens Up Studies Within Single Cells
Scientists have developed a method for simultaneously imaging and identifying dozens of molecules within individual cells.
Scientists Learn How to Predict Plant Size
VIB and UGent scientists have developed a new method which allows them to predict the final size of a plant while it is still a seedling.
New Device Reduces Time to Diagnose Infections
A new diagnostic device created by a collaborative team of UA engineers and scientists may significantly reduce the amount of time necessary to diagnose tissue infections.
Surprising Mechanism Behind Antibiotic-Resistant Bacteria Uncovered
Now, scientists at TSRI have discovered that the important human pathogen Staphylococcus aureus, develops resistance to this drug by “switching on” a previously uncharacterized set of genes.
Researchers Develop qPCR Prognosis Test for NSCLC Patients
A nine-gene molecular prognostic index (MPI) for patients with early-stage non-small cell lung cancer (NSCLC) was able to provide accurate survival stratification and could potentially inform the use of adjuvant therapy in patients struggling with the disease.
Genome Wide Annotation of Primary miRNAs Reveals Novel Mechanisms
Researchers have devised a strategy for genome-wide annotation of primary miRNA transcripts, providing extensive new annotations in human and mouse, and shedding light on mechanisms of regulation of microRNA gene expression.
‘Fishing Expedition’ Nets Nearly Tenfold Increase in Number of Sequenced Virus Genomes
Newly developed computational tool finds 12,500 genomes of viruses that infect microbes.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos