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High Yield RNA Isolation without the Paraffin Removal Step

Published: Wednesday, May 15, 2013
Last Updated: Wednesday, May 15, 2013
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AMSBIO offers a range of kits and reagents for the extraction (isolation) of high quality RNA from FFPE tissues and LCM samples that can be used not only for PCR but also microarray applications.

Using AMSBIO ExpressArt® RNA isolation technology it is now possible to achieve high RNA yield and maximum sequence length from FFPE or LCM samples without the paraffin removal step. ExpressArt® RNA technology overcomes traditional limitations placed on research due to limited sample size, RNA degradation and interference from the crosslinking in FFPE samples.

ExpressArt® FFPE RNA-ready isolation and mRNA amplification technology allows researcher to isolate higher quality and quantities of RNA from samples previously unsuitable for gene expression analysis. The kits includes patented de-cross linking technology which is uniquely able to isolate high quality RNA from old, archival FFPE samples (10 years old or more). The FFPE RNAready Kit provides special lysis and incubation conditions for optimal RNA recovery and improved template activity. Deparaffinisation of the tissue samples is followed by homogenisation and lysis by incubation with proteinase K and detergents. The lysis buffer is supplemented with the nucleic acid analogue NucleoGuard, which acts as universal inhibitor of RNases and displaces tightly bound RNA from high-molecular-weight aggregates, resulting in efficient release of RNA and preservation of the RNA quality. RNA solubilisation is followed by an innovative demodification step to reverse formalin induced cross-links and this enables the purified RNA to deliver maximal performance as template in reverse transcription reactions and subsequent downstream assays.

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