Integrated DNA Technologies (IDT) features a “Core Concepts” article highlighting useful tips for multiplex qPCR users in the recently released April 3.2 issue of the IDT DECODED newsletter.
The article provides recommendations and step-by-step considerations from the IDT qPCR experts that will help researchers to make informed decisions about their multiplex qPCR experiments, including: general set-up, assay design, and whether or not multiplex qPCR is the right choice for existing applications.
Multiplex qPCR can be an extremely attractive amplification method, allowing scientists to quickly measure the expression levels of several targets simultaneously, enhancing experimental efficiency.
However, successful multiplexing requires attention to experimental design. For example, target amplification can be influenced by numerous factors such as gene expression levels, primer interactions, and competition for reaction reagents.
IDT produced these guidelines as a valuable reference for new and experienced users of multiplex qPCR, providing key considerations and technique fundamentals to maximize approach success.
The full piece, along with real scientific application examples and links to the useful IDT online primer design and analysis tools, can be found in the 3.2 issue of DECODED.