Bio-Rad Laboratories, Inc. today announced the launch of its PrimePCR probe assays for quantitative real-time PCR (qPCR). The new assays for human and mouse genomes set the bar for specificity, sensitivity, and linear dynamic range.
Bio-Rad has again partnered with Biogazelle, a qPCR data analysis and services company, to design probe assays for qPCR gene expression analysis. The fluorescently labeled oligonucleotide probes were designed to complement Bio-Rad’s high-specificity PCR primer assays, enabling multiplex assays and eliminating the need for melt curve analysis.
The primer pair of each probe assay has been wet-lab validated following strict assay performance standards for amplification efficiency, specificity, sensitivity, and linear dynamic range. These specifications help researchers meet the MIQE (minimum information for publication of quantitative real-time PCR experiments) guidelines.
The new probe assays are validated for use with iScript™ advanced cDNA synthesis kit for RT-qPCR and SsoAdvanced™ universal probes supermix for superior performance.
The assays are available with probes labeled with FAM, HEX, or TEX 615 fluorophores, and each includes a gene-specific synthetic qPCR template designed as a positive control. The probe assays can be used with CFX real-time PCR detection systems and data analysis software, streamlining PCR data collection and analysis.
PrimePCR probe assays are available in 500, 1,000, and 2,500 reaction sizes.