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Single Cell Whole Genome Amplification
GE Healthcare

The analysis of genomes at the single cell level offers unprecedented biological insights in diverse fields such as cancer research, immunology & microbiology. This poster discusses the a technology for amplification of genomic DNA form a single cell, that provides utmost sensitivity, accuracy & robustness. GE Healthcare Life Sciences had previously developed method of multiple strand displacement amplification (MDA) by Phi29 DNA polymerase

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System-Specific Periodicity in qPCR Data and its Impact on Quantitation
Joel Tellinghuisen,1 Stefan Roediger,2 Thomas Volksdorf,3 and Andrej-Nikolai Spiess3

In some commercial qPCR instruments, the fluorescence signal shows reaction-to-reaction periodicity in all cycles, and this translates into periodicity in threshold-based Cq markers.

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A Microwave/Microfluidics Integrated Label-Free Detection and Content Sensing System for Point-of-Care Applications
Gurkan Yesiloz, Muhammed S. Boybay, Carolyn L. Ren

This poster presents a droplet-based microfluidic device integrated with a microwave wave sensor, which is also a heater, for simultaneous sensing and heating of individual nanoliter-sized droplets.

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Rapid Detection of Somatic Mutations in Cancer Genes
Michael J. Powell et. al.,

Rapid higly sensitive detection of tumor gene mutations in DNA derived from FFPE or plasma samples can be achieved with QClamp PCR technology.

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Accurate Normalization of Grain Samples Using Pressure-Based Volume Measurement Technology
Bill Gigante, John Thomas Bradshaw, Tanya Knaide

A common challenge experienced by sample screening facilities is identifying individual sample quantity prior to DNA extraction. Accurately identifying sample quantity allows the user to pipette exact amounts of extraction buffer into each well.

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DHPLC Technology as a High-throughput Detection of Mutations in a Durum Wheat TILLING Population
Colasuonno P.1, Incerti O. 1, Lozito M.L. 1, Sbalzarini M. 2, Zaccagna P. 2, Papadimitriou S. 2, Blanco A. 1, Gadaleta A. 1

This study is a beautiful example of DHPLC technology application and shows an alternative tool to current strategies of SNP detection based on genotyping array.

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IDENTIFICATION AND DIFERENTIATION OF Verticillium SPECIES WITH PCR MARKERS AND SEQUENCING OF ITS REGION
Taja Jesenicnik, Nataša Štajner, Jernej Jakše, Sebastijan Radišek and Branka Javornik

The genus Verticillium is a group of ascomycete fungi, including plant-pathogenic species capable of affecting the vasculature of many agricultural crops, and therefore causes major economic losses worldwide. In 2011, a new taxonomic classification of the genus was proposed, which is now referred to as Verticillium sensu stricto, comprising ten species: V. dahliae V. albo-atru, V. alfalfae, V. longisporum, V. nonalfalfae, V. tricorpus, V. zaregamsianum, V. nubilum, V. isaacii and V. klebahnii. <

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Expression Profiling of Circulating Tumor Cells: a Prognostic and Predictive Biomarker in Cancer.
Mikael Kubista, Robert Sjöback,Marie Jindrichova, Eva Rohlova, Vendula Novosadová, Siegfrid Hauch, Bahriye Aktas, Mitra Tewes,Maren Bredemeier, and SabineKasimir-Bauer

We show non-responders to treatment among breast cancer patients can be identified by expression profiling of circulating tumor cells

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Detection in Environmental Samples of Enteroagregative Escherichia coli, stx-producing Escherichia coli and stx-converting Bacteriophage by RT- PCR: Preliminary Data
Mura Elia, Noli Alessia Caterina, Rossi Maria Lucia, Terrosu Giovanni, Fadda Antonio

The aim of this work was to collect epidemiological information concerning the presence of Enteroaggregative Escherichia coli, stx-producing Escherichia coli and stx2a-converting bacteriophage, in ruminants faeces.

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Effect of exposure to stress conditions on propidium monoazide (PMA) - qPCR based enumeration of Campylobacter in broiler carcass rinses
A. Duarte1,2*, N. Botteldoorn2 ,W. Coucke2, S. Denayer2, K. Dierick2, and M. Uyttendaele1

Evaluation and comparison of a developed PMA-qPCR Campylobacter enumeration method with the reference method, after exposure of the broiler rinse artificially contaminated with the bacteria to stress conditions.

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Scientific News
Analysing 10,000 Cells Simultaneously
New techniquethat traps 10,000 cells on a single chip has potential for cancer screening for individuals.
$1M NIH Grant to Refine PCR Based Cancer Test
Researchers at Cornell University, Weill Cornell Medicine, the University of California, San Francisco, and the Infectious Diseases Institute in Kampala, Uganda, recieve a four-year, $1 million grant to hone technology for a quick, in-the-field diagnosis of Kaposi's sarcoma — a cancer frequently related to HIV infections.
Genetic Tug of War Before Cells Decide Fate
Researchers report that as developing blood cells are triggered by genetic signals firing on and off, a 'tug of war' occurs.
Linkage Biosciences Awarded NHS Contract
Comapny announces that it has been awarded a four-year contract by NHS Blood and Transplant (NHSBT) in the UK for implementation of the LinkSeq™ Real-Time PCR HLA typing product.
Understanding Tumor Evolution
Study provides insight into tumor evolution; may point to improved diagnosis and treatment.
Frankfurter Fraud: Finding Out What’s In Your Hot Dog
Scientists have developed a technique to test the meat content of Frankfurters.
How Cloud Connectivity Can Combat the Reproducibility Crisis
This infographic explains the reproducibility crisis, and how cloud connectivity can help overcome this problem.
Keeping Cells in Shape to Fight Sepsis
Boosting levels of a protein that controls the shape and activity of a crucial group of white blood cells improves survival and recovery chances during sepsis.
This 3D Printer Can Detect Infectious Diseases
Researchers at AI Biosciences, Inc. have repurposed 3D printers into machines capable of performing sample preparation and DNA amplification.
Natera, UCSF Announce Collaboration
Joint research program to study DNA markers of kidney transplant rejection.
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