|Rapid Detection of Somatic Mutations in Cancer Genes|
Michael J. Powell et. al.,
Rapid higly sensitive detection of tumor gene mutations in DNA derived from FFPE or plasma samples can be achieved with QClamp PCR technology.
|DHPLC Technology as a High-throughput Detection of Mutations in a Durum Wheat TILLING Population|
Colasuonno P.1, Incerti O. 1, Lozito M.L. 1, Sbalzarini M. 2, Zaccagna P. 2, Papadimitriou S. 2, Blanco A. 1, Gadaleta A. 1
This study is a beautiful example of DHPLC technology application and shows an alternative tool to current strategies of SNP detection based on genotyping array.
|IDENTIFICATION AND DIFERENTIATION OF Verticillium SPECIES WITH PCR MARKERS AND SEQUENCING OF ITS REGION|
Taja Jesenicnik, Nataša Štajner, Jernej Jakše, Sebastijan Radišek and Branka Javornik
The genus Verticillium is a group of ascomycete fungi, including plant-pathogenic species capable of affecting the vasculature of many agricultural crops, and therefore causes major economic losses worldwide. In 2011, a new taxonomic classification of the genus was proposed, which is now referred to as Verticillium sensu stricto, comprising ten species: V. dahliae V. albo-atru, V. alfalfae, V. longisporum, V. nonalfalfae, V. tricorpus, V. zaregamsianum, V. nubilum, V. isaacii and V. klebahnii. <
|Expression Profiling of Circulating Tumor Cells: a Prognostic and Predictive Biomarker in Cancer.|
Mikael Kubista, Robert Sjöback,Marie Jindrichova, Eva Rohlova, Vendula Novosadová, Siegfrid Hauch, Bahriye Aktas, Mitra Tewes,Maren Bredemeier, and SabineKasimir-Bauer
We show non-responders to treatment among breast cancer patients can be identified by expression profiling of circulating tumor cells
|Detection in Environmental Samples of Enteroagregative Escherichia coli, stx-producing Escherichia coli and stx-converting Bacteriophage by RT- PCR: Preliminary Data|
Mura Elia, Noli Alessia Caterina, Rossi Maria Lucia, Terrosu Giovanni, Fadda Antonio
The aim of this work was to collect epidemiological information concerning the presence of Enteroaggregative Escherichia coli, stx-producing Escherichia coli and stx2a-converting bacteriophage, in ruminants faeces.
|Effect of exposure to stress conditions on propidium monoazide (PMA) - qPCR based enumeration of Campylobacter in broiler carcass rinses|
A. Duarte1,2*, N. Botteldoorn2 ,W. Coucke2, S. Denayer2, K. Dierick2, and M. Uyttendaele1
Evaluation and comparison of a developed PMA-qPCR Campylobacter enumeration method with the reference method, after exposure of the broiler rinse artificially contaminated with the bacteria to stress conditions.
|Digital PCR to Determine the Number of Transcripts from Single Neurons after Patch-clamp Recording|
Nóra Faragó1,2, Ágnes K. Kocsis3, Sándor Lovas3, Gábor Molnár3, Márton Rózsa3, Viktor Szemenyei3, Ágnes Zvara2, Gábor Tamás3, László G Puskás1,2
Whole-cell patch-clamp recording enables detecting electrophysiological signals from neurons, and RNA can be harvested into the patch pipette from the cells.We have optimized a dPCR protocol for determining exact transcript numbers in single neurons after patch-clamp recording by using dPCR based on high-density nanocapillary PCR.
|On-chip quantification of miRNA using digital droplet PCR|
Q. Cai1, R.S. Wiederkehr1, B. Jones1, B. Majeed1, T. Stakenborg1, P. Fiorini1, L. Lagae1, M Tsukuda2, T. Matsuno2, I. Yamashita2
miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.
|A multianalyte algorithm PCR-based blood test outperforms single analyte ELISA-based blood tests for neuroendocrine tumor detection|
Mark Kidd, Irvin M Modlin, Daniele Alaimo, Stephen Callahan, Nancy Teixiera, Lisa Bodei, Ignat Drozdov
In a prospective study, in age-/sex- and ethnicity-matched patients and controls (n=82), a 51 panel multigene blood transcript test (NETest) was identified to be significantly more sensitive and accurate (>93%) than any single analyte assay (Chromogranin A, Pancreastatin or Neurokinin A) for neuroendocrine tumor detection.
|Stealth-Adapted Viruses and Viteria: Insights into Virus Construction, Replication and Potential Therapies|
W. John Martin
There is an increasing incidence of diseases with accompanying signs and symptoms of brain damage. These include neurological and psychiatric illnesses, childhood behavioral disorders, and such common conditions as chronic fatigue, Gulf War Syndrome, so-called “chronic Lyme disease”, and many cancers. Altogether, these diseases have an enormous social impact.
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