|A Novel Cell-Free DNA Extraction System for Cancer Diagnostics|
Amanda Fan, Alex Yeo, Yin Kum Ng, Elian Rakhmanaliev, Pramila Ariyaratne, Charlie Lee, Gerd Michel, Tatiana Ivanova
We developed a magnetic bead-based cfDNA extraction kit and integrated it into the automated qPCR- and NGS-based IVD workflows. In this study we are compering this magnetic beads-based cfDNA extraction system with a column-based method.
|Integrated Next-Generation Sequencing and qPCR Workflow in Clinical Diagnostics|
Elian Rakhmanaliev, Tatiana Ivanova, Gerd Michel
Combined automated qPCR and NGS Sentosa workflow is a reliable and efficient in vitro diagnostics (IVD) tool for the detection and/or quantitation of a wide range of bacterial and viral pathogens as well as gene mutations.
|Single Cell Whole Genome Amplification|
The analysis of genomes at the single cell level offers unprecedented biological insights in diverse fields such as cancer research, immunology & microbiology. This poster discusses a technology for amplification of genomic DNA from a single cell, that provides utmost sensitivity, accuracy & robustness. GE Healthcare Life Sciences had previously developed a method of multiple strand displacement amplification (MDA) by Phi29 DNA polymerase.
|System-Specific Periodicity in qPCR Data and its Impact on Quantitation|
Joel Tellinghuisen,1 Stefan Roediger,2 Thomas Volksdorf,3 and Andrej-Nikolai Spiess3
In some commercial qPCR instruments, the fluorescence signal shows reaction-to-reaction periodicity in all cycles, and this translates into periodicity in threshold-based Cq markers.
|A Microwave/Microfluidics Integrated Label-Free Detection and Content Sensing System for Point-of-Care Applications|
Gurkan Yesiloz, Muhammed S. Boybay, Carolyn L. Ren
This poster presents a droplet-based microfluidic device integrated with a microwave wave sensor, which is also a heater, for simultaneous sensing and heating of individual nanoliter-sized droplets.
|Rapid Detection of Somatic Mutations in Cancer Genes|
Michael J. Powell et. al.,
Rapid higly sensitive detection of tumor gene mutations in DNA derived from FFPE or plasma samples can be achieved with QClamp PCR technology.
|Accurate Normalization of Grain Samples Using Pressure-Based Volume Measurement Technology|
Bill Gigante, John Thomas Bradshaw, Tanya Knaide
A common challenge experienced by sample screening facilities is identifying individual sample quantity prior to DNA extraction. Accurately identifying sample quantity allows the user to pipette exact amounts of extraction buffer into each well.
|DHPLC Technology as a High-throughput Detection of Mutations in a Durum Wheat TILLING Population|
Colasuonno P.1, Incerti O. 1, Lozito M.L. 1, Sbalzarini M. 2, Zaccagna P. 2, Papadimitriou S. 2, Blanco A. 1, Gadaleta A. 1
This study is a beautiful example of DHPLC technology application and shows an alternative tool to current strategies of SNP detection based on genotyping array.
|IDENTIFICATION AND DIFERENTIATION OF Verticillium SPECIES WITH PCR MARKERS AND SEQUENCING OF ITS REGION|
Taja Jesenicnik, Nataša Štajner, Jernej Jakše, Sebastijan Radišek and Branka Javornik
The genus Verticillium is a group of ascomycete fungi, including plant-pathogenic species capable of affecting the vasculature of many agricultural crops, and therefore causes major economic losses worldwide. In 2011, a new taxonomic classification of the genus was proposed, which is now referred to as Verticillium sensu stricto, comprising ten species: V. dahliae V. albo-atru, V. alfalfae, V. longisporum, V. nonalfalfae, V. tricorpus, V. zaregamsianum, V. nubilum, V. isaacii and V. klebahnii. <
|Expression Profiling of Circulating Tumor Cells: a Prognostic and Predictive Biomarker in Cancer.|
Mikael Kubista, Robert Sjöback,Marie Jindrichova, Eva Rohlova, Vendula Novosadová, Siegfrid Hauch, Bahriye Aktas, Mitra Tewes,Maren Bredemeier, and SabineKasimir-Bauer
We show non-responders to treatment among breast cancer patients can be identified by expression profiling of circulating tumor cells
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