The FastStart TaqMan® Probe Master (Rox) is a ready-to-use, 2x-concentrated master reagent mix for hydrolysis-probe-based qPCR and two-step qRT-PCR assays. When used with an appropriate real-time PCR instrument and hydrolysis probes, FastStart TaqMan®Probe Master (Rox) allows very sensitive detection and quantification of defined DNA sequences. Do not use this product with the LightCycler® Instruments. In principle, the FastStart TaqMan®Probe Master (Rox) can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich. However, you would need to adapt your detection protocol to the reaction conditions of the particular real-time PCR instrument used and design a specific hydrolysis probe and PCR primers for each target. See the Operator's Manual of your real-time PCR instrument for general recommendations. Combine this master mix with our Transcriptor First Strand cDNA Synthesis Kit to achieve excellent results in two-step qRT-PCR. Insist on the Transcriptor First Strand cDNA Synthesis Kit - designed and function-tested for qPCR, and efficient with all real-time PCR instruments.