The FastStart Universal Probe Master (Rox) is a ready-to-use reagent mix that simplifies the preparation of reactions for qPCR and two-step qRT-PCR. In combination with a real-time PCR instrument, suitable PCR primers, and a hydrolysis probe, FastStart Universal Probe Master (Rox) enables very sensitive detection and quantification of defined DNA sequences. This product is not designed for use with the LightCycler® Instruments. In principle, the FastStart Universal Probe Master (Rox) can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich. Combine this master mix with our Transcriptor First Strand cDNA Synthesis Kit to achieve excellent results in two-step qRT-PCR. Insist on the Transcriptor First Strand cDNA Synthesis Kit -designed and function-tested for qPCR, and efficient with all real-time PCR instruments.