Satellite Banner
Scientific Community
Become a Member | Sign in
Home>Products>This Product

5’/3’ RACE Kit, 2nd Generation

Product Description
Robust and reliable amplification of long unknown sequence by RACE.
Obtaining a full-length cDNA is of critical importance for structural and expression studies. The amplification of DNA sequences from a messenger RNA template between a defined internal site and unknown sequences of either the 3' or the 5' end of the mRNA are often referred to as RACE (rapid amplification of cDNA ends), “anchored” PCR, or “one-sided” PCR. Transcriptor Reverse Transcriptase is supplied with the kit to transcribe full-length cDNA up to 14 kb in length and, due to the thermostability of Transcriptor Reverse Transcriptase (up to 65°C), to work with GC-rich templates with high secondary structure. In addition, with Transcriptor Reverse Transcriptase, high sensitivity can be achieved. This results in highly efficient cDNA synthesis and the generation of long RACE products.
Purification of the first-strand cDNA is performed with the High Pure PCR Product Purification Kit. Recombinant Terminal Transferase is used to add a homopolymeric A-tail to the 3' end of the cDNA. The use of poly(A) tail decreases the likelihood of inappropriate trunction by the oligo dT-anchor primer, and due to the weaker A/T binding compared to the G/C binding, longer stretches of A residues are required before the oligo dT-anchor primer will bind to an internal site and truncate the amplification product.
Tailed cDNA is amplified by PCR using a gene-specific primer and the oligo dT-anchor primer. The obtained cDNA is further amplified by a second PCR using a nested specific primer and the PCR anchor primer. As a result, the RACE products can be cloned into an appropriate vector for subsequent characterization studies.
Product 5’/3’ RACE Kit, 2nd Generation
Company Roche - Products Listing
Price Request a quote
More Information View company product page
Catalog Number 03353621001
Quantity 1 kit (10 reactions)
Company Logo

Roche - Products Listing
Roche Diagnostics Corporation Roche Applied Science P.O. Box 50414 9115 Hague Road Indianapolis, IN 46250-0414 US

Tel: +1-800-428-5433
Fax: +1-800-428-2883

Scientific News
Ancient Viral Molecules Essential for Human Development
Genetic material from ancient viral infections is critical to human development, according to researchers at the Stanford University School of Medicine.
Measuring microRNAs in Blood to Speed Cancer Detection
A simple, ultrasensitive microRNA sensor holds promise for the design of new diagnostic strategies and, potentially, for the prognosis and treatment of pancreatic and other cancers.
Best Test to Diagnose Strangles in Horses Identified
New research by Dr. Ashley Boyle of New Bolton Center’s Equine Field Service team shows that the best method for diagnosing Strangles in horses is to take samples from a horse’s guttural pouch and analyze them using a loop-mediated amplification (LAMP) polymerase chain reaction (PCR) test.
Tardigrade's Are DNA Master Thieves
Tardigrades, nearly microscopic animals that can survive the harshest of environments, including outer space, hold the record for the animal that has the most foreign DNA.
Rapid, Portable Ebola Diagnostic
Scientists confirmed the efficiency of the novel Ebola detection method in field trials.
Detecting When Hormone Treatment for Breast Cancer Stops Working
Scientists have developed a highly sensitive blood test that can spot when breast cancers become resistant to standard hormone treatment, and have demonstrated that this test could guide further treatment.
Packaging and Unpacking of the Genome
New research improves understanding of the importance of histone replacement.
New Way to Find DNA Damage
University of Utah chemists devised a new way to detect chemical damage to DNA that sometimes leads to genetic mutations responsible for many diseases, including various cancers and neurological disorders.
How Different Treatments for Crohn's Effect the Microbiome
Different treatments for Crohn's disease in children affects their gut microbes in distinct ways, which has implications for future development of microbial-targeted therapies for these patients, according to a study led by researchers from the Perelman School of Medicine at the University of Pennsylvania.
Charting the 'Genomic Biography' of Leukemia
A new study by scientists at Dana-Farber Cancer Institute and the Broad Institute of MIT and Harvard offers a glimpse of the wealth of information that can be gleaned by combing the genome of a large collection of leukemia tissue samples.

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos