Corporate Banner
Satellite Banner
qPCR
Scientific Community
 
Become a Member | Sign in
Home>Products>This Product
  Products


Shrimp WSSV Detection Test Kit

Product Description
The MaxSignal® Shrimp WSSV Detection Kit uses an end-point Polymerase Chain Reaction (PCR) assay to screen for WSSV in fresh or frozen shrimp or other potentially infectious material. White Spot Syndrome Virus (WSSV) is claimed to be the most serious viral pathogen that affects cultivated shrimp worldwide. The MaxSignal® Shrimp WSSV Detection Kit contains 2 pairs of oligonucleotide primers targeting nested regions of the WSSV VP466 gene (accession # EF564812), which encodes a structural protein located in the nucleocapsid. The WSSV amplicon produced using the WSSV-483 primers in the kit is 483 bp and the amplicon produced using the WSSV-388 primers is 388 bp. These WSSV amplicons have no significant homology to any other viral sequence. The detection limit for the synthetic WSSV template included in the kit is approximately 50 copies using the standard 1-step PCR protocol, and approximately 5 copies using the 2-step nested PCR protocol. For detection of WSSV in infected shrimp, positive signals have been obtained using the 1-step PCR protocol with input amounts of approximately 50 femtograms (50 x 10-15 gm) of total nucleic acid extracted from shrimp that were experimentally infected with high levels of the WSSV virus. The Shrimp Positive Control primers included in the kit were designed to the actin 1 gene (Accession # AF100986) from Penaeus monodon and will produce an amplicon of 249 bp from P monodon and also from Penaeus vannamei. The Shrimp Positive Control primers are used to demonstrate successful extraction of DNA that is able to support PCR. Input sample for this kit is total DNA or total nucleic acid extracted from solid tissue or derived from liquid tissues such as hemolymph; purification of viral particles for analysis is not required. To maximize user convenience, the reagents for PCR (buffer, dNTPs, and thermostable polymerase) are provided as a 5x MasterMix. The MasterMix also contains components to allow the PCR to be directly loaded for gel analysis, without adding gel-loading solution.
Product Shrimp WSSV Detection Test Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 1201
Quantity 100 PCR rxns
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
Top 10 Life Science Innovations of 2016
2016 has seen the release of some truly innovative products. To help you digest these developments, The Scientist have listed their top picks for the year.
Using Cancer Cells' Mass to Predict Treatment Response
A device has been developed that can detect changes in cell mass at a minute scale.
NVIDIA Awards $400k to Trailblazers in Cancer Research
NVIDIA Foundation furthers research that could lead to new and more targeted treatments with investments.
Malaria Parasite Evades Rapid Test Detection in Children
A study at the University of North Carolina found that gene deletion poses a threat to Malaria eradication efforts.
Working With Advanced Nanoimagers
Oxford Nanoimaging report on the work of early-adopters for their Nanoimager technology at the MRC Centre for Molecular Bacteriology and Infection.
History of Cells Told Through MEMOIR
MEMOIR technique developed by CalTech researchers enables the life history of cells to be read.
Nanoscale ‘Muscles’ Powered by DNA
Scientists have developed nanoscale "muscles" to integrate with custom DNA, that can force the material to bend, curl and flip.
Dissecting Bacterial Infections at the Single-Cell Level
Researchers have used single-cell analysis technology to provide new insight into the Salmonella infection process.
Peer Review is in Crisis, But Should be Fixed, Not Abolished
After the time to get the science done, peer review has become the slowest step in the process of sharing studies, and some scientists have had enough.
Cancer Stem Cells Fuel Tumor Growth
Mass. General, Broad Institute team finds strong evidence that cancer stem cells are important drivers of tumors in patients.

Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
4,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,300+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!