Corporate Banner
Satellite Banner
qPCR
Scientific Community
 
Become a Member | Sign in
Home>Products>This Product
  Products


Listeria Extraction and Detection Test Kit

Product Description
The MaxSignal® Listeria Extraction and Detection Kit uses an end-point PCR assay to screen for the presence of Listeria bacteria in potentially infectious material. The purification method is based on lysis of Listeria pellets at high temperature followed by solid-phase extraction of the DNA on silica filters. The procedure can be carried out in less than 30 min using common laboratory equipment. The use of purified Listeria DNA allows for increased sensitivity compared to Listeria detection methods that use samples consisting of unpurified bacterial lysates, because the DNA is concentrated into a smaller volume for use in volume-limited PCR reactions. The kit also contains 3 synthetic templates for use as Internal Amplification Controls. The synthetic templates have primer binding sites for one of the Outer Primer Pairs (A, B, or C) and an internal region consisting of a plasmid DNA sequence. The plasmid sequence is the same in all 3 templates. The control templates are typically added to the PCRs along with the sample DNA, to create positive amplification controls. Amplification of the expected size amplicons from the synthetic templates proves that the sample DNA did not contain inhibitors of PCR. To maximize user convenience, the reagents for PCR (buffer, dNTPs, and thermostable polymerase) are provided as a 5x MasterMix. The MasterMix also contains components to allow the PCR to be directly loaded for gel analysis, without adding gel-loading solution. The kit includes a molecular weight “ladder” for analysis of results from experimental samples. The kit contains 6 pairs of oligonucleotide primers targeting outer and nested regions of three different types of Listeria sample: 2 primer pairs specific for all Listeria species (Primer Pairs A outer and A nested); 2 primer pairs specific for Listeria monocytogenes (Primer Pairs B outer and B nested); and 2 primer pairs specific for Listerial moncytogenes isolate 4b (Primer Pairs C outer and C nested). The primers were designed against Listeria target genes. The kit also includes reagents and materials for purification of Listeria DNA from liquid cultures.

Product Listeria Extraction and Detection Test Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 1202
Quantity 50 Extractions & 100 PCR rxns
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
New Method Promises to Speed Development of Food Crops
A new study addresses a central challenge of transgenic plant development: how to reliably evaluate whether genetic material has been successfully introduced.
Study Validates Analysis of Copy Number Variation in Miniaturized Reaction Volumes
Data shows that accurate and reproducible CNV results can be produced with IntelliQube using the Array Tape® consumable.
Faster Drug Discovery?
Startup develops more cost-effective test for assessing how cells respond to chemicals.
Edited Stem Cells Offer Hope of Precision Therapy for Blindness
Findings raise the possibility of treating blinding eye diseases using a patient's own corrected cells as replacement tissue.
Parallel Single-Cell Profiling
New single-cell genomics protocol allows researchers to study links between DNA modifications (methylation) and the activity of a gene.
Pathogens Found in Iceman's Gut
Scientists discover Helicobacter pylori in the contents of Ötzi’s stomach along with some unexpected insights into the coexistence of man and bacterium.
Diagnosing Cancer from a Single Drop of Blood
What if a physician could effectively diagnose cancer from one drop of a patient’s blood?
Tracing a Cellular Family Tree
New technique allows tracking of gene expression over generations of cells as they specialize.
Accelerating Protein Evolution
A new tool enables researchers to test millions of mutated proteins in a matter of hours or days, speeding the search for new medicines, industrial enzymes and biosensors.
Ancient Viral Molecules Essential for Human Development
Genetic material from ancient viral infections is critical to human development, according to researchers at the Stanford University School of Medicine.

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!