Corporate Banner
Satellite Banner
qPCR
Scientific Community
 
Become a Member | Sign in
Home>Products>This Product
  Products


Listeria Extraction and Detection Test Kit

Product Description
The MaxSignal® Listeria Extraction and Detection Kit uses an end-point PCR assay to screen for the presence of Listeria bacteria in potentially infectious material. The purification method is based on lysis of Listeria pellets at high temperature followed by solid-phase extraction of the DNA on silica filters. The procedure can be carried out in less than 30 min using common laboratory equipment. The use of purified Listeria DNA allows for increased sensitivity compared to Listeria detection methods that use samples consisting of unpurified bacterial lysates, because the DNA is concentrated into a smaller volume for use in volume-limited PCR reactions. The kit also contains 3 synthetic templates for use as Internal Amplification Controls. The synthetic templates have primer binding sites for one of the Outer Primer Pairs (A, B, or C) and an internal region consisting of a plasmid DNA sequence. The plasmid sequence is the same in all 3 templates. The control templates are typically added to the PCRs along with the sample DNA, to create positive amplification controls. Amplification of the expected size amplicons from the synthetic templates proves that the sample DNA did not contain inhibitors of PCR. To maximize user convenience, the reagents for PCR (buffer, dNTPs, and thermostable polymerase) are provided as a 5x MasterMix. The MasterMix also contains components to allow the PCR to be directly loaded for gel analysis, without adding gel-loading solution. The kit includes a molecular weight “ladder” for analysis of results from experimental samples. The kit contains 6 pairs of oligonucleotide primers targeting outer and nested regions of three different types of Listeria sample: 2 primer pairs specific for all Listeria species (Primer Pairs A outer and A nested); 2 primer pairs specific for Listeria monocytogenes (Primer Pairs B outer and B nested); and 2 primer pairs specific for Listerial moncytogenes isolate 4b (Primer Pairs C outer and C nested). The primers were designed against Listeria target genes. The kit also includes reagents and materials for purification of Listeria DNA from liquid cultures.

Product Listeria Extraction and Detection Test Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 1202
Quantity 50 Extractions & 100 PCR rxns
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
Benchtop Automation Trends
Gain a better understanding of current interest in and future deployment of benchtop automated systems.
Measuring the Abundance of Extremely Rare Mutations
Researchers from Rutgers University demonstrate the use of multiplex real-time PCR assays to measure the abundance of extremely rare mutations associated with cancer.
Harnessing Helpful Microbes
Seeking to further harness microbes’ many uses, the federal government has launched the National Microbiome Initiative (NMI) to “foster the integrated study of microbiomes across different ecosystems.”
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
New Tool Could Change How Infectious Diseases Are Diagnosed
Scientists at the University of Utah School of Medicine, ARUP Laboratories, and IDbyDNA, Inc., have developed ultra-fast, meta-genomics analysis software called Taxonomer that dramatically improves the accuracy and speed of pathogen detection.
Finding Zika One Paper Disc At A Time
A novel, inexpensive method for detecting the Zika virus could help slow spread of outbreak, and potentially other future pandemic diseases.
Rapid Diagnosis of Bacterial Infections
Mass. General-developed compact system could shorten diagnostic time from days to hours, bring testing to point of care.
Monovar Drills Down Into Cancer Genome
Rice, MD Anderson develop program to ID mutations in single cancer cells.
Biomarkers for Profiling Prostate Cancer Patients
Exiqon A/S has announced the publication of validation of prognostic microRNA biomarkers for the aggressiveness of prostate cancer in independent cohorts.
Gene Analysis System Could Accelerate Pace of Research on the Space Station
The WetLab-2 system will enable spaceflight genomic studies involving a wide variety of biospecimen types in the unique microgravity environment of space.

SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!