Satellite Banner
Technology Networks Header
Thursday, December 18, 2014
Technology Networks
 
Register | Sign in
Home Page > Videos > In-vivo Single Cell Transcript Analyses for Systems Modelling
  Videos

Return

In-vivo Single Cell Transcript Analyses for Systems Modelling
SELECTBIO

To investigate the importance of minimal residual disease of BCR-ABL expressing cells chronic myelogenous leukemia (CML) was assessed using single cell molecular analytical approaches. CML is believed to occur as a consequence of the clonal expansion of leukemic stem cells and to be maintained by an expanding population of hematopoietic stem cells that have acquired a BCR-ABL fusion gene. Recent studies indicate that primitive CML cells are less responsive to tyrosine kinase inhibitors and are a reservoir for the emergence of tyrosine kinase resistant subclones. It also has been reported that BCR-ABL mediated cell adhesion may be involved in post-therapy residual disease of CML. The natural heterogeneity seen across both attached and unattached BCR-ABL expressing populations has been investigated. The study developed a means to utilize flow-assisted cell sorting of cell lines expressing BCR-ABL to derive individual attached and unattached cell sub-populations. Using a homogenous extraction procedure, cells individually flow sorted into microtitre plates were subjected to combinations of ABL and BCR-ABL RT qPCR, and also phosphorylated BCR-ABL protein was assessed using the proximity ligation assay and revealed the existence of lowly and highly BCR-ABL expressing cell line populations. The implications of this study will be discussed along with newly derived systems models and in-vivo sample procedures employing AFM.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Did You Hear The One About The Two Businessmen On A Plane?
EquipNet’s vision ‘to revolutionize the way companies manage their surplus assets’ was first conceived at 30,000 feet somewhere between Chicago and Boston in 1999. Here we learn more about the company's first 15 years.
Developing a Noninvasive Test for Endometriosis
UCSF researchers identify patterns of genetic activity that could help in early detection of disorder.
CDC Develops a New, Faster Lab Test for Enterovirus D68
Confirmed cases will appear to rise as agency accelerates specimen testing.
NanoString, Brigham and Women’s Hospital Collaborate
Research collaboration to accelerate translation of genomic discoveries into clinical diagnostics in oncology.
Unlocking The Non-Coding Half of Human Genome
Texas A&M biologists unlock non-coding half of human genome with novel DNA sequencing technique.
Improving Trout Resistance to a Deadly Disease
A highly sensitive real-time PCR test has been developed to measure F. psychrophilum in fish tissue.
Blood Test To Diagnose Depression
Test identifies nine blood markers tied to depression; predicts who will benefit from therapy.
Redefining High Risk Patients With Stage II Colon Cancer
microRNA-21 identified as an independent prognostic biomarker.
More People Testing Positive for Chikungunya Virus in the U.S.
Clinical testing volume and positivity rate both grew after the first case of the infectious disease spread to Western Hemisphere this year.
Novel rhPCR Unravels Gene Expression
Method enables identification and analysis of highly similar alternatively spliced transcripts.
Scroll Up
Scroll Down
Skyscraper Banner
SELECTBIO Market Reports
LabTube - Videos for the Scientific Community
eposters - The Online Journal of Scientific Posters
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn