Satellite Banner
Technology Networks Header
Thursday, December 18, 2014
Technology Networks
 
Register | Sign in
Home Page > Videos > Extreme PCR: Robust, Efficient Amplification in < 1 Min
  Videos

Return

Extreme PCR: Robust, Efficient Amplification in < 1 Min
SELECTBIO

Rapid-cycle PCR was introduced in 1990, reducing 2-4 hours of amplification to 10-15 min. Since that time, incremental progress has been made in reducing amplification times below 10 minutes, although yield and PCR efficiency often suffer at higher speeds. We now introduce, “Extreme PCR”, a method that combines <2 s thermal cycles with chemistry modifications to enhance the completion of annealing and extension segments. Specifically, primer and polymersase concentrations are increased so that primer annealing and complete extension near 100% efficiency each cycle. Cycle times of less than 1 s were obtained for PCR products less than 100 bp, resulting in specific, high-yield amplification as demonstrated by gels and melting curves. In addition, we introduce a new method for measuring polymerase activity under PCR conditions. Performed on real-time instruments, the effect of various components on extension rates reveal that monovalent cations, most Tm depressants, secondary structure, probes and dyes inhibit extension, while Mg++, temperature, GC content, and DMSO have local maxima. By studying the effect of different PCR components on extension rate, amplification speed can be optimized.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Did You Hear The One About The Two Businessmen On A Plane?
EquipNet’s vision ‘to revolutionize the way companies manage their surplus assets’ was first conceived at 30,000 feet somewhere between Chicago and Boston in 1999. Here we learn more about the company's first 15 years.
Developing a Noninvasive Test for Endometriosis
UCSF researchers identify patterns of genetic activity that could help in early detection of disorder.
CDC Develops a New, Faster Lab Test for Enterovirus D68
Confirmed cases will appear to rise as agency accelerates specimen testing.
NanoString, Brigham and Women’s Hospital Collaborate
Research collaboration to accelerate translation of genomic discoveries into clinical diagnostics in oncology.
Unlocking The Non-Coding Half of Human Genome
Texas A&M biologists unlock non-coding half of human genome with novel DNA sequencing technique.
Improving Trout Resistance to a Deadly Disease
A highly sensitive real-time PCR test has been developed to measure F. psychrophilum in fish tissue.
Blood Test To Diagnose Depression
Test identifies nine blood markers tied to depression; predicts who will benefit from therapy.
Redefining High Risk Patients With Stage II Colon Cancer
microRNA-21 identified as an independent prognostic biomarker.
More People Testing Positive for Chikungunya Virus in the U.S.
Clinical testing volume and positivity rate both grew after the first case of the infectious disease spread to Western Hemisphere this year.
Novel rhPCR Unravels Gene Expression
Method enables identification and analysis of highly similar alternatively spliced transcripts.
Scroll Up
Scroll Down

SELECTBIO Market Reports
LabTube - Videos for the Scientific Community
eposters - The Online Journal of Scientific Posters
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn