Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Integrated Omics Analysis
Studying multi-omics promises to give a more holistic picture of the organism and its place in its ecosystem, however despite the complexities involved those within the field are optimistic.
Unravelling the Role of Key Genes and DNA Methylation in Blood Cell Malignancies
Researchers from the University of Nebraska Medical Center have demonstrated the role of Dnmt3a in safeguarding normal haematopoiesis.
Salford Lung Study - The First Real World Clinical Trial
In this podcast, we learn about the Salford Lung Study and its potential to revolutionize the way we assess new drugs and treatments around the world.
Point of Care Diagnostics - A Cautious Revolution
Advances in molecular biology, coupled with the miniaturization and improved sensitivity of assays and devices in general, have enabled a new wave of point-of-care (POC) or “bedside” diagnostics.
Mass Spec Technology Drives Innovation Across the Biopharma Workflow
With greater resolving power, analytical speed, and accuracy, new mass spectrometry technology and techniques are infiltrating the biopharmaceuticals workflow.
Structure of Primary Cannabinoid Receptor is Revealed
The findings provide key insights into how natural and synthetic cannabinoids including tetrahydrocannabinol —a primary chemical in marijuana—bind at the CB1 receptor to produce their effects.
Illumina Contributes to ClinVar Database
The contribution includes variants of all classifications, from pathogenic to benign, identified during interpretation of whole genome sequences generated in the CLIA-certified, CAP-accredited Illumina Clinical Services Laboratory.
Overlooked Molecules Could Revolutionise our Understanding of the Immune System
Researchers have discovered that around one third of all the epitopes displayed for scanning by the immune system are a type known as ‘spliced’ epitopes.
Study Finds Key Regulator in Pulmonary Fibrosis
Researchers identify an enzyme that could open the way to therpies for chronic fatal lung disease.
Signaling Pathway Could Be Key to Improved Osteoporosis Treatment
Inhibition of SIK2 enzyme both stimulates bone formation and reduces bone breakdown in animal model.
Scroll Up
Scroll Down

Bacterial Detection and Live/Dead Discrimination by Flow Cytometry
Bookmark and Share

BD Biosciences

Flow cytometry, a technique first applied to eukaryotic cells, has been adapted to the analysis of viability, metabolic state, and antigenic markers of bacteria.1-4 In particular, flow cytometry can be readily applied to the enumeration of viable bacteria in a sample.

Live cells have intact membranes and are impermeable to dyes such as propidium iodide (PI), which only leaks into cells with compromised membranes. Thiazole orange* (TO) is a permeant dye and enters all cells, live and dead, to varying degrees. With gram-negative organisms, depletion of the lipopolysaccharide layer with EDTA greatly facilitates TO uptake. Thus a combination of these two dyes provides a rapid and reliable method for discriminating live and dead bacteria. If enumeration of the bacteria is important, BD Liquid Counting Beads (BD Biosciences, San Jose, CA), a flow cytometry bead standard, can be used to accurately quantify the number of live, dead, and total bacteria in a sample.

Further Information

Related Content

BD Receives FDA Clearance for Gastroenteritis Test
The FDA-cleared BD MAX™ Enteric Bacterial Panel for use on the BD MAX System.
Monday, May 12, 2014
BD Collaborates with CME Medical
Collaboration to increase coverage in the UK and Ireland and to provide expanded specialist support to its growing Saf-T-Intima™ customer base.
Sunday, January 12, 2014
BD Announces Approval of Safety Integrated IV Catheter System for Subcutaneous Therapy in Europe
BD Saf-T-Intima™ increases patient comfort and in-dwell times while enabling cost savings and reducing the risk of sharps injuries and infection.
Tuesday, July 23, 2013
BD Helps to Support the Implementation of New Sharps Instruments in Healthcare Regulations
Legislation in place to protect healthcare workers from sharps injuries as a result of new EU Directive on sharps injury prevention.
Thursday, June 13, 2013
BD Acquires Sirigen Group Limited
Expands BD Biosciences' life science research reagent platform.
Wednesday, September 05, 2012
New IVD Product is Designed to Improve Prevention and Control of GBS in Newborns
BD MAX™ GBS assay receives FDA clearance and CLIA moderate complexity test categorization.
Wednesday, March 28, 2012
BD Diagnostics and Bruker Collaborate to Improve Microbial Identification and Antimicrobial Susceptibility Testing
BD Diagnostics and Bruker Daltonics Inc. have announced an international co-development and co-marketing collaboration that will promote an emerging, integrated approach to bacterial and fungal identification and antimicrobial susceptibility testing.
Friday, February 11, 2011
BD Biosciences to Expand Manufacturing in Durham, North Carolina
The expansion of Treyburn facility will consolidate and increase U.S. manufacturing for BD Biosciences’ Discovery Labware product line.
Monday, May 14, 2007
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,100+ scientific videos