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Big Genetics in BC: The American Society for Human Genetics 2016 Meeting
Themes at this year's meeting ranged from the verification, validation, and sharing of data, to the translation of laboratory findings into actionable clinical results.
Stem Cells in Drug Discovery
Potential Source of Unlimited Human Test Cells, but Roadblocks Remain.
Cancer Genetics: Key to Diagnosis, Therapy
When applied judiciously, cancer genetics directs caregivers to the right drug at the right time, while sparing patients of unnecessary or harmful treatments.
Transporting Microscopic Cargo Between Human Cells
Scientists have developed a virus-inspired delivery system for material transport between cells.
Tissue Damage Is Key for Cell Reprogramming
Researchers have shown tissue damage is important for cells to return to an embryonic state for cell reprogramming.
Metabolite Promotes Cancer Cell Transformation
Researchers have identified a metabolite that promotes cancer cell transformation and colorectal cancer spread.
Improving Drug Production with Computer Model
A model has been developed that can be used to improve and accelerate the production of biotherapeutics, cancer drugs, and vaccines.
Bird Flu Confirmed in the Netherlands
An outbreak of H5 avian influenza was confirmed in the Flevoland province of the Netherlands.
Pasteurised Bacterium Reduces Obesity and Diabetes
Researchers have discovered that an intestinal bacterium provides a lasting effect on the intestinal barrier.
Turning Off Asthma Attacks
Researchers discover a critical cellular “off” switch for the inflammatory immune response that causes asthma attacks.
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Complementing Next Generation Sequencing Technologies With Agilent’s SureSelect DNA Capture Array
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Agilent Technologies

Massively parallel DNA sequencing technologies are of pivotal importance in genome biology and medicine, as they can potentially enable comprehensive and systematic evaluation of genetic variation. Currently, these sequencing technologies are geared toward sequencing whole genomes. A broader adoption of these technologies requires a more cost-effective method with higher throughput and greater versatility than PCR—a method such as target enrichment, the targeted resequencing of multiple discrete genomic regions of interest. To validate the use of Agilent DNA microarrays for target enrichment, Agilent collaborated with the laboratory of Dr. Greg Hannon (Cold Spring Harbor Laboratory) to capture exonic regions relevant to a breast cancer sequencing study. We targeted 0.025% of the human genome, using an Agilent 244K array of 60-mer probes to capture approximately 1,287 discrete genomic regions.
The captured DNA was then released and sequenced. Various hybridization conditions were tested, ultimately obtaining a 2,700-fold enrichment of sequencing reads within targeted regions. The system was seen to be effective, with sequencing reads covering over 99.8% of the targeted regions and 98% of the targeted bases with at least one read and with a normalized average per-base read depth of 27 per million 32-base reads. These results confi rm that Agilent DNA microarrays can provide a rapid and effective solution for targeted sequencing of genomic regions of interest.

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