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Liquid Biopsies: Utilization of Circulating Biomarkers for Minimally Invasive Diagnostics Development
Market Trends in Biofluid-based Liquid Biopsies: Deploying Circulating Biomarkers in the Clinic. Enal Razvi, Ph.D., Managing Director, Select Biosciences, Inc.
Lab-on-a-Chip Offers Promise for TB and Asthma Patients
A device to mix liquids using ultrasonics is the first and most difficult component in a miniaturized system for low-cost analysis of sputum from patients with pulmonary diseases such as tuberculosis and asthma.
Intracellular Microlasers Could Allow Precise Labeling of up to a Trillion Individual Cells
MGH investigators have induced structures incorporated within individual cells to produce laser light at wavelengths that differ based on the size, shape and composition of each microlaser, allowing precise labeling of individual cells.
Real-Time Imaging of Lung Lesions During Surgery
Targeted molecular agents cause lung adenocarcinomas to fluoresce during surgery, according to pilot report.
Watching a Tumour Grow in Real-Time
Researchers from the University of Freiburg have gained new insight into the phases of breast cancer growth.
Protein Related to Long Term Traumatic Brain Injury Complications Discovered
NIH-study shows protein found at higher levels in military members who have suffered multiple TBIs.
Childhood Cancer Cells Drain Immune System’s Batteries
Cancer cells in neuroblastoma contain a molecule that breaks down a key energy source for the body’s immune cells, leaving them too physically drained to fight the disease.
Urine Proteins Point to Early-Stage Pancreatic Cancer
A combination of three proteins found at high levels in urine can accurately detect early-stage pancreatic cancer, researchers at the BCI have shown.
Researcher Discovers Trigger of Deadly Melanoma
New research sheds light on the precise trigger that causes melanoma cancer cells to transform from non-invasive cells to invasive killer agents, pinpointing the precise place in the process where "traveling" cancer turns lethal.
New Vaccine For Chlamydia to Use Synthetic Biology
Prokarium Ltd, a biotechnology company developing transformational oral vaccines, have announced new funding from SynbiCITE, the UK’s Innovation and Knowledge Centre for Synthetic Biology.
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Complementing Next Generation Sequencing Technologies With Agilent’s SureSelect DNA Capture Array
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Agilent Technologies

Massively parallel DNA sequencing technologies are of pivotal importance in genome biology and medicine, as they can potentially enable comprehensive and systematic evaluation of genetic variation. Currently, these sequencing technologies are geared toward sequencing whole genomes. A broader adoption of these technologies requires a more cost-effective method with higher throughput and greater versatility than PCR—a method such as target enrichment, the targeted resequencing of multiple discrete genomic regions of interest. To validate the use of Agilent DNA microarrays for target enrichment, Agilent collaborated with the laboratory of Dr. Greg Hannon (Cold Spring Harbor Laboratory) to capture exonic regions relevant to a breast cancer sequencing study. We targeted 0.025% of the human genome, using an Agilent 244K array of 60-mer probes to capture approximately 1,287 discrete genomic regions.
The captured DNA was then released and sequenced. Various hybridization conditions were tested, ultimately obtaining a 2,700-fold enrichment of sequencing reads within targeted regions. The system was seen to be effective, with sequencing reads covering over 99.8% of the targeted regions and 98% of the targeted bases with at least one read and with a normalized average per-base read depth of 27 per million 32-base reads. These results confi rm that Agilent DNA microarrays can provide a rapid and effective solution for targeted sequencing of genomic regions of interest.

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