Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Liquid Biopsies: Miracle Diagnostic or Next New Fad?
Thanks to the development of highly specific gene-amplification and sequencing technologies liquid biopsies access more biomarkers relevant to more cancers than ever before.
Flu Vaccine May Reduce Risk of Death For Type 2 Diabetes Patients
Researchers at Imperial College London have suggested that the vaccine may have substantial benefits for patients with long-term conditions.
Cancer Gene-Drug Combinations Ripe for Precision Medicine
The study aims to expand the number of cancer gene mutations that can be paired with a precision therapy.
Porphyrins as Catalysts in Scalable Organic Reactions
This review covers the most relevant scalable porphyrin-catalysed procedures, showing how these compounds represent broad applications in chemistry.
CDC Updates Zika Recommendations
CDC has issued updated Zika recommendations and guidance for healthcare providers with a focus on sexual transmission.
Exploiting Malaria’s Achilles’ Heel
Researchers have uncovered an Achilles' heel in malaria's anti-drug treatment arsenal that could lead to a disease cure.
Genome of 6000-Year-Old Barley Sequenced
Researchers have successfully sequenced the genome of Chalcolithic barley grains for the first time.
3D Models May Yield Ovarian Cancer Insights
Researchers are developing new tools to decipher ovarian cancer developments through a 3D printing technology.
Targeting BRAF Mutations in Thyroid Cancer
Treating metastatic thyroid cancer patients harboring a BRAF mutation with vemurafenib showed anti-tumor activity in a third of patients.
Plant Compounds Fight Together Against Colon Cancer
Research shows treating colon cancer cells with curcumin, then silymarin is more effective than treatment with each individually.
Scroll Up
Scroll Down

The ELISAONE ™ Assay Performed on the POLARstar Omega from BMG LABTECH
Bookmark and Share


BMG LABTECH

ELISAONE assays use a traditional immuno-sandwich format, but with a major difference. The analyte and both antibodies are added to the ELISAONE assay microplate at the same time, allowing solution-phase binding.

In contrast to other ELISA formats, the target-specific antibodies needed for the assay are not pre-bound to the microplate itself. The binding of antibodies to the analyte takes place in solution, allowing for efficient binding. This not only reduces assay times, but also affords several other benefits to the user. As the antibodies are not fixed to the plate, assays for several different targets can be performed in different wells on the same microplate, side-by-side. Another important benefit is reagent lifetime – many ELISA kits need to be discarded one month after opening, largely because the antibody-coated plates are often not stable after opening. With ELISAONE however, the expensive assay reagents are in reusable bottles, and last a long time after the ELISAONE microplate has expired. The inexpensive microplate can simply be discarded and another ordered, as they’re all identical.

This application note details how the ELISAONE assays for EGF, TNFα and IL-2 developed by TGR BioSciences, were used to demonstrate the robustness and high sensitivity of the ELISAONE technology.  

Further Information

Related Content

25 Years of Revolutionary Microplate Reader Technology at the LS2
BMG LABTECH exhibits at the biggest Swiss Life Sciences Meeting for the first time.
Tuesday, December 16, 2014
BMG LABTECH is Attending ARABLAB 2012
Company will be exhibiting at ARABLAB 2012 from March 26-29th. Visit Booth #408 to get a personal introduction to the SPECTROstar Nano and the POLARstar Omega microplate readers.
Thursday, March 22, 2012
BMG LABTECH is a Diamond Sponsor of the First Annual SLAS 2012
Stop by booth #921 to see The Microplate Reader Company and to see what is new with microplate reader technology.
Thursday, January 26, 2012
BMG LABTECH Announces First FP User Group Meeting
The meeting brings together a wide range of speakers with extensive experience of Fluorescence Polarization techniques.
Monday, April 26, 2010
BMG LABTECH Announces First FP User Group Meeting
Company announces their inaugural UK Fluorescence Polarisation user group meeting on the 23rd of April 2010.
Monday, April 19, 2010
BMG LABTECH, World Leader in Microplate Reader Technology, is proud to be a Bronze Sponsor of the SBS Conference 2010.
Visit us at booth 817 and join us at our workshops to see latest innovations in microplate reading technology including the ultimate HTS Microplate Reader, PHERAstar FS
Tuesday, March 30, 2010
BMG Labtech Joins ALSSA
The ALSSA - Analytical & Life Science Systems Association - Board of Directors recently approved applications for membership submitted by BMG LABTECH, Inc. Based in Cary, North Carolina.
Thursday, March 25, 2010
DLReady Certification for the Omega Series of Microplate Readers
DLReady certification, awarded by Promega Corp., validates an instrument that has passed the strict performance levels and standards required for the Dual-Luciferase Reporter (DLR) assay system.
Monday, December 03, 2007
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,800+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!