Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Sorting Through Cellular Statistics
Aaron Dinner, professor in chemistry, and his graduate student Herman Gudjonson are trying to read the manual of life, DNA, as part of the Dinner group’s research into bioinformatics—the application of statistics to biological research.
Playing 'Tag' with Pollution lets Scientists See Who's It
Using a climate model that can tag sources of soot from different global regions and can track where it lands on the Tibetan Plateau, researchers have determined which areas around the plateau contribute the most soot — and where.
Women’s Immune System Genes Operate Differently from Men’s
A new technology reveals that immune system genes switch on and off differently in women and men, and the source of that variation is not primarily in the DNA.
Long Telomeres Associated with Increased Lung Cancer Risk
Genetic predisposition for long telomeres predicts increased lung adenocarcinoma risk.
First Artificial Ribosome Designed
Researchers at the University of Illinois at Chicago and Northwestern University have engineered a tethered ribosome that works nearly as well as the authentic cellular component, or organelle, that produces all the proteins and enzymes within the cell.
High-Resolution 3D Images Reveal the Muscle Mitochondrial Power Grid
NIH mouse study overturns scientific ideas on energy distribution in muscle.
Expanding the Brain
A team of researchers has identified more than 40 new “imprinted” genes, in which either the maternal or paternal copy of a gene is expressed while the other is silenced.
Identifying a Key Growth Factor in Cell Proliferation
Researchers discover that aspartate is a limiter of cell proliferation.
Study Uncovers Target for Preventing Huntington’s Disease
Scientists from Cardiff University believe that a treatment to prevent or delay the symptoms of Huntington’s disease could now be much closer, following a major breakthrough.
Paving the way to Better Ovarian Cancer Diagnosis
Aïcha BenTaieb will present her invention for automated identification of ovarian cancer’s many subtypes at an international conference this fall.
Scroll Up
Scroll Down

The ELISAONE ™ Assay Performed on the POLARstar Omega from BMG LABTECH
Bookmark and Share


BMG LABTECH

ELISAONE assays use a traditional immuno-sandwich format, but with a major difference. The analyte and both antibodies are added to the ELISAONE assay microplate at the same time, allowing solution-phase binding.

In contrast to other ELISA formats, the target-specific antibodies needed for the assay are not pre-bound to the microplate itself. The binding of antibodies to the analyte takes place in solution, allowing for efficient binding. This not only reduces assay times, but also affords several other benefits to the user. As the antibodies are not fixed to the plate, assays for several different targets can be performed in different wells on the same microplate, side-by-side. Another important benefit is reagent lifetime – many ELISA kits need to be discarded one month after opening, largely because the antibody-coated plates are often not stable after opening. With ELISAONE however, the expensive assay reagents are in reusable bottles, and last a long time after the ELISAONE microplate has expired. The inexpensive microplate can simply be discarded and another ordered, as they’re all identical.

This application note details how the ELISAONE assays for EGF, TNFα and IL-2 developed by TGR BioSciences, were used to demonstrate the robustness and high sensitivity of the ELISAONE technology.  

Further Information

Related Content

25 Years of Revolutionary Microplate Reader Technology at the LS2
BMG LABTECH exhibits at the biggest Swiss Life Sciences Meeting for the first time.
Tuesday, December 16, 2014
BMG LABTECH is Attending ARABLAB 2012
Company will be exhibiting at ARABLAB 2012 from March 26-29th. Visit Booth #408 to get a personal introduction to the SPECTROstar Nano and the POLARstar Omega microplate readers.
Thursday, March 22, 2012
BMG LABTECH is a Diamond Sponsor of the First Annual SLAS 2012
Stop by booth #921 to see The Microplate Reader Company and to see what is new with microplate reader technology.
Thursday, January 26, 2012
BMG LABTECH Announces First FP User Group Meeting
The meeting brings together a wide range of speakers with extensive experience of Fluorescence Polarization techniques.
Monday, April 26, 2010
BMG LABTECH Announces First FP User Group Meeting
Company announces their inaugural UK Fluorescence Polarisation user group meeting on the 23rd of April 2010.
Monday, April 19, 2010
BMG LABTECH, World Leader in Microplate Reader Technology, is proud to be a Bronze Sponsor of the SBS Conference 2010.
Visit us at booth 817 and join us at our workshops to see latest innovations in microplate reading technology including the ultimate HTS Microplate Reader, PHERAstar FS
Tuesday, March 30, 2010
BMG Labtech Joins ALSSA
The ALSSA - Analytical & Life Science Systems Association - Board of Directors recently approved applications for membership submitted by BMG LABTECH, Inc. Based in Cary, North Carolina.
Thursday, March 25, 2010
DLReady Certification for the Omega Series of Microplate Readers
DLReady certification, awarded by Promega Corp., validates an instrument that has passed the strict performance levels and standards required for the Dual-Luciferase Reporter (DLR) assay system.
Monday, December 03, 2007
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!