Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Mass Spec Technology Drives Innovation Across the Biopharma Workflow
With greater resolving power, analytical speed, and accuracy, new mass spectrometry technology and techniques are infiltrating the biopharmaceuticals workflow.
One Step Closer to Precision Medicine for Chronic Lung Disease Sufferers
A study led by University of North Carolina at Chapel Hill, and National Jewish Health, has provided evidence of links between SNPs and known COPD blood protein biomarkers.
Gene Regulation in Brain May Explain Repetitive Behaviors in Rett Syndrome Patients
The research could be a key step in developing treatments to eliminate symptoms that drastically impair the quality of life in Rett patients.
Heart Arrhythmia Caused by Mosaic of Mutant Cells
Researchers have solved the genetic mystery of an infant suffering from heart arrhythmia.
Iron Nanoparticles Make Immune Cells Attack Cancer
Researchers accidentally discover that nanoparticles invented for anemia treatment can trigger the immune system’s ability to destroy tumor cells.
Crispr Toolbox Expanded By Protein
Researchers have shown a newly discovered CRISPR protein has two distinct RNA cutting activities.
Atmosphere Acidity Minimised to Preindustrial Levels
Sheet ice study shows acidic pollution of the atmosphere has now almost returned to preindustrial levels.
New Therapeutic Target for Crohn’s Disease
A promising new target for drugs that treat IBD has been identified along with a possible biomarker for IBD severity.
Culex Mosquitoes Do Not Transmit Zika
A study of the Culex species mosquito appears to show that the species does not transmit Zika virus.
Uncovering Water Bear Resilience
A protein identified in water bears can protect DNA of human cells from lethal doses of radiation damage.
Scroll Up
Scroll Down

Micro-Volume Purity Assessment of Nucleic Acids using A260/A280 Ratio and Spectral Scanning
Bookmark and Share


BioTek Instruments

The application note highlights the ability to determine the concentration and purity of analyte in a sample volume as low as 2 μL using spectrophotometry. Additionally, examples of the ability to perform absorption spectral scans on low volume samples are provided. The spectral data can be useful for detection of common residual contaminants from biomolecule isolation methods by comparison to spectra of highly purified sample.

While the usefulness of the A260/A280 ratio for determining the purity of the sample is understood, it is important to recognize the limitations of relying on the A260/A280 ratio as a sole means for validation.

Further Information

Related Content

BioTek Introduces New General Manager in Germany
Company appoints Dr. Frank Siefert who will be responsible for managing day-to-day operations in Germany.
Tuesday, November 03, 2015
BioTek to Exhibit at Neuroscience 2015
Join BioTek Instruments as they demonstrate the latest microplate innovations at the SfN’s 45th annual meeting. Neuroscience 2015 will be held October 17-21 at McCormick Place in Chicago, IL. BioTek will showcase their products at booth #1809.
Tuesday, September 29, 2015
BioTek Readers Receive BellBrook Certifications
Company’s multi-mode microplate readers earn Transcreener HTS Assay certifications.
Friday, September 11, 2015
BioTek Readers Receive Promega DLReady™ Certification
Promega’s Dual-Luciferase© Reporter System demonstrate both companies’ common commitment.
Friday, August 07, 2015
BioTek Instruments Receives 5x5x5 Growth Award for Technology
BioTek Instruments has been recognized as one of the 5x5x5 Growth Award winners for 2015.
Wednesday, July 29, 2015
BioTek Expands in the United Kingdom
Company expands their ongoing investment with the opening of their new UK Office in Swindon.
Friday, June 05, 2015
Biotek Named as Finalist for Top Employer Award
The company have been announced as a finalist for the Secretary of Defense Employer Support Freedom Award.
Thursday, May 21, 2015
BioTek Nominated for Department of Defense’s Top Employer Award
BioTek Instruments been nominated for the 2015 Secretary of Defense Employer Support Freedom Award by the Employer Support of the Guard and Reserve (ESGR), a Department of Defense (DoD) office.
Tuesday, March 31, 2015
BioTek Receives 2014 Supplier of the Year Award from Fisher Scientific
BioTek was presented with the award at Thermo Fisher Scientific’s annual meeting in Phoenix.
Wednesday, March 25, 2015
BioTek to Receive 2014 Governor’s Excellence in Worksite Wellness Award
Award recognises worksites in Vermont that promote the health and wellness of their employees.
Wednesday, January 21, 2015
BioTek Receives Two Awards from ESGR
Company has received the Patriot Award and the Above and Beyond Award.
Friday, August 22, 2014
BioTek Receives Extraordinary New Product Line Award from Fisher
Company receives award for Cytation 3 Cell Imaging Multi-Mode Reader.
Tuesday, March 04, 2014
BioTek Announces Establishment of Japanese Office
BioTek Japan will be fully functional by July 1, 2013.
Monday, June 10, 2013
BioTek Responds to Growing AP Market Needs with New Taiwan Office
New office will focus on increasing BioTek's brand presence in Taiwan.
Tuesday, May 14, 2013
BioTek Wins Governor's Award for Outstanding Workplace Safety
BioTek receives the award by Governor Peter Shumlin at the 9th Annual Vermont Workplace Safety Conference.
Thursday, April 18, 2013
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!