Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
RNAi Screening Trends
Understand current trends and learn which application areas are expected to gain in popularity over the next few years.
The Genetic Roots of Adolescent Scoliosis
Scientists at the RIKEN Center for Integrative Medical Sciences in collaboration with Keio University in Japan have discovered a gene that is linked to susceptibility of Scoliosis.
A Gene-Sequence Swap Using CRISPR to Cure Haemophilia
For the first time chromosomal defects responsible for hemophilia have been corrected in patient-specific iPSCs using CRISPR-Cas9 nucleases
Experimental MERS Vaccine Shows Promise in Animal Studies
A two-step regimen of experimental vaccines against Middle East respiratory syndrome (MERS) prompted immune responses in mice and rhesus macaques, report National Institutes of Health scientists who designed the vaccines.
New Tool Uses 'Drug Spillover' to Match Cancer Patients with Treatments
Researchers have developed a new tool that improves the ability to match drugs to disease: the Kinase Addiction Ranker (KAR) predicts what genetics are truly driving the cancer in any population of cells and chooses the best "kinase inhibitor" to silence these dangerous genetic causes of disease.
Understanding the Molecular Origin of Epigenetic Markers
Researchers at IRB Barcelona discover the molecular mechanism that determines how epigenetic markers influence gene expression.
HIV Susceptibility Linked to Little-Understood Immune Cell Class
High levels of diversity among immune cells called natural killer cells may strongly predispose people to infection by HIV, and may be driven by prior viral exposures, according to a new study.
Diagnostic Test Developed for Enterovirus D68
researchers at Washington University School of Medicine in St. Louis have developed a diagnostic test to quickly detect enterovirus D68 (EV-D68), a respiratory virus that caused unusually severe illness in children last year.
How a Kernel Got Naked and Corn Became King
Ten thousand years ago, a golden grain got naked, brought people together and grew to become one of the top agricultural commodities on the planet.
Sweet Revenge Against Superbugs
A special type of synthetic sugar could be the latest weapon in the fight against superbugs.
Scroll Up
Scroll Down

Determination of Antioxidant potential using an Oxygen Radical Absorbance Capacity (ORAC) Assay with Synergy™ H4
Bookmark and Share


BioTek Instruments

Oxidative damage to living organisms has been associated with several disease states as well as aging. The sources of reactive oxygen species (ROS) are varied and can lead to the formation of toxic compounds within organisms. ROS sources such as cellular respiration, interaction of biomolecules with ionizing radiation, and dedicated cellular pathways for ROS formation as a protective mechanism ensure chronic exposure of living organisms to ROS species.

A proper balance must be maintained between oxidants and antioxidants to ensure the ubiquitous ROS species do not become deleterious to the organism. Disruption of any one of these mechanisms can result in an imbalance and ensuing damage to a number of critical components required to maintain cellular homeostasis. Thus, there is interest in the ability to accurately determine the antioxidant capabilities of foods, cosmetics, dietary supplements and pharmaceutical agents.

While several methods exist to measure total antioxidant capacity, ORAC has emerged as a low cost method suitable for high throughput automation in a microplate format. The ORAC assay relies on free radical damage to a fluorescent probe, most commonly fluorescein, caused by an oxidizing reagent resulting in a loss of fluorescent intensity over time.The resultant damage can then be correlated with the amount of oxidant present. Conversely, inhibition of oxidative damage to the fluorescent probe can be correlated with the antioxidant capacity of a compound acting as a free radical scavenger.

This application note demonstrates the ability to perform the assay in a 96-well microplate format amenable to higher throughput platforms.

Further Information

Related Content

BioTek Instruments Receives 5x5x5 Growth Award for Technology
BioTek Instruments has been recognized as one of the 5x5x5 Growth Award winners for 2015.
Wednesday, July 29, 2015
BioTek Expands in the United Kingdom
Company expands their ongoing investment with the opening of their new UK Office in Swindon.
Friday, June 05, 2015
Biotek Named as Finalist for Top Employer Award
The company have been announced as a finalist for the Secretary of Defense Employer Support Freedom Award.
Thursday, May 21, 2015
BioTek Nominated for Department of Defense’s Top Employer Award
BioTek Instruments been nominated for the 2015 Secretary of Defense Employer Support Freedom Award by the Employer Support of the Guard and Reserve (ESGR), a Department of Defense (DoD) office.
Tuesday, March 31, 2015
BioTek Receives 2014 Supplier of the Year Award from Fisher Scientific
BioTek was presented with the award at Thermo Fisher Scientific’s annual meeting in Phoenix.
Wednesday, March 25, 2015
BioTek to Receive 2014 Governor’s Excellence in Worksite Wellness Award
Award recognises worksites in Vermont that promote the health and wellness of their employees.
Wednesday, January 21, 2015
BioTek Receives Two Awards from ESGR
Company has received the Patriot Award and the Above and Beyond Award.
Friday, August 22, 2014
BioTek Receives Extraordinary New Product Line Award from Fisher
Company receives award for Cytation 3 Cell Imaging Multi-Mode Reader.
Tuesday, March 04, 2014
BioTek Announces Establishment of Japanese Office
BioTek Japan will be fully functional by July 1, 2013.
Monday, June 10, 2013
BioTek Responds to Growing AP Market Needs with New Taiwan Office
New office will focus on increasing BioTek's brand presence in Taiwan.
Tuesday, May 14, 2013
BioTek Wins Governor's Award for Outstanding Workplace Safety
BioTek receives the award by Governor Peter Shumlin at the 9th Annual Vermont Workplace Safety Conference.
Thursday, April 18, 2013
BioTek Germany Certified in Accordance with EN ISO 13485
Certification guarantees that all of BioTek’s products and services satisfy the requirements and comply with the currently valid legislation.
Monday, December 17, 2012
BioTek Among 5x5x5 Growth Award Special Honorees
Annual award is presented to Vermont companies experiencing outstanding growth in one of five business categories.
Friday, September 21, 2012
BioTek Receives European Patent for Microplate Washer Technology
Ultrasonic Advantage uses unique, integrated ultrasonic cleaning to reduce protein and salt crystal build-up.
Tuesday, June 12, 2012
BioTek Expands Asia Pacific Office
BioTek Instruments, Inc., continues their commitment to the Asia Pacific region with the expansion of their Singapore office.
Thursday, May 10, 2012
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!