Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Big Genetics in BC: The American Society for Human Genetics 2016 Meeting
Themes at this year's meeting ranged from the verification, validation, and sharing of data, to the translation of laboratory findings into actionable clinical results.
Stem Cells in Drug Discovery
Potential Source of Unlimited Human Test Cells, but Roadblocks Remain.
Automated Low Volume Dispensing Trends
Gain a better understanding of the current and future market requirements for fully automated LVD systems.
Personality Traits, Psychiatric Disorders Linked to Specific Genomic Locations
Researchers have unearthed genetic correlations between personality traits and psychiatric disorders.
Forensic 3D Documentation of Skin Injuries
In this study, the validity of using photogrammetry for documenting injuries in a pathological context was demonstrated.
3-D Printed Dog’s Nose Improves Vapor Detection
By mimicking how dogs get their whiffs, a team of government and university researchers have demonstrated that “active sniffing” can improve by more than 10 times the performance of current technologies that rely on continuous suction to detect trace amounts of explosives and other contraband.
New Markers for Forensic Body-fluid Identification
University of Bonn researchers have successfully identified specific Micro-RNA signatures to help forensically identify body fluids.
Genetics Control Regenerative Properties Of Stem Cells
Researchers define how genetic factors control regenerative properties of blood-forming stem cells.
Major Neuroscience Initiative Launched
Tianqiao and Chrissy Chen Institute invest $115 million to further expand neuroscience research, while Caltech construct $200 million biosciences complex.
Making It Personal
Cancer vaccine linked to increased immune response against leukemia cells.
Scroll Up
Scroll Down

Next Generation Sequencing Method for Illumina TruSeq DNA Sample Preparation Protocol on the Hamilton STAR
Bookmark and Share


Hamilton

Next Generation Sequencing (NGS) has accelerated the sequencing of genomes and this technology has a major role to play in the biomedical research. The simplicity of NGS library preparation, the ability to make these libraries from tiny input amounts of DNA, the elimination of a bacterial cloning intermediate and the development of data-analysis approaches to support the increasing size of the sequence datasets have been invaluable in the advancement of NGS. This application note describes the automation of the Illumina TruSeq DNA sample preparation on the Hamilton STAR . The data presented includes PicoGreen assay and Sybr E-Gel results for comparison of library construction using automated and manual methods, Bioanalyzer and KAPA assay results and data from Illumina HiSeq. Automation allowed processing 96 samples simultaneously in less than 4 hrs. Sample recovery yields higher by 50% were obtained with automated compared to manual method. The sample preparation provided average 80% reads on target from Illumina HiSeq.

Further Information

Related Content

Hamilton Storage Technologies Opens 52,000-Square-Foot Facility in Franklin, Massachusetts
Innovative open floor plan helps life science business address growing market demands.
Tuesday, January 22, 2013
Hamilton Robotics and French Police Scientifique Publish Paper Describing Large-Scale Production of Genetic Profiles
More than 300,000 profiles have already been processed on the Hamilton platforms and stored in French Database.
Wednesday, March 10, 2010
Hamilton Star Liquid Handler Installation at the Diamond Light Source MPL Laboratory
Hamilton Robotics has installed a STAR liquid handler at the commissioned Membrane Protein Crystallography Laboratory at the Diamond Light Source near Didcot in Oxfordshire.
Monday, June 11, 2007
Automation of Adherent Cell Culture Maintenance
Hamilton, Life & Brain and University of Bonn will jointly develop a system for the automated culture of primary cells, cell lines and embryonic stem cells.
Monday, May 14, 2007
Introduction of an Anti Droplet Control Pipette
HAMILTON introduces ADC, for pipetting volatile organic solvents, with pressure sensors in the pipetting channel to monitor each pipetting step.
Monday, December 11, 2006
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
4,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,300+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!