Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
Kitchen Utensils Can Spread Bacteria Between Foods
In a recent study researchers found that produce that contained bacteria would contaminate other produce items through the continued use of knives or graters—the bacteria would latch on to the utensils commonly found in consumers' homes and spread to the next item.
Exploring the Causes of Cancer
Queen's research to understand the regulation of a cell surface protein involved in cancer.
Safer, Faster Way To Remove Pollutants From Water
Using nanoparticles filled with enzymes proves more effective than current methods.
Drug May Prevent Life-Threatening Muscle Loss in Advanced Cancers
New data describes how an experimental drug can stop life-threatening muscle wasting (cachexia) associated with advanced cancers and restore muscle health.
Ancient Viral Molecules Essential for Human Development
Genetic material from ancient viral infections is critical to human development, according to researchers at the Stanford University School of Medicine.
Novel Tumor Treatment
In the first published results from a $386,000 National Cancer Institute grant awarded earlier this year, a paper by Scott Verbridge and Rafael Davalos has been published.
Speeding Up the Process of Making Vaccines
System uses a freeze-dry concept to develop "just-add-water" solution.
Chemical Design Made Easier
Rice University scientists prepare elusive organocatalysts for drug and fine chemical synthesis.
New Analysis Technique for Chiral Activity in Molecules
Professor Hyunwoo Kim of the Chemistry Department and his research team have developed a technique that can easily analyze the optical activity of charged compounds by using nuclear magnetic resonance (NMR) spectroscopy.
Scroll Up
Scroll Down

BRD4 Bromodomain 1 Inhibitor Screening Using a Homogeneous Proximity Assay
Bookmark and Share

BioTek Instruments

Epigenetics refers to a dynamic set of processes that regulate transcription of the genome, some involving chemical modification of histone proteins. Histones associate with DNA to form nucleosomes, which bundle to form chromatin and chromosomes. Histones contain a flexible N-terminus tail, which protrudes from the nucleosome and is the site for epigenetic modifications. Modifications to amino acid residues in the histone tail include the addition and removal of functional groups, such as methyl and acetyl groups by specific enzymes. Other proteins affect epigenitic changes by reading whether the histone tail has been modified. A bromodomain protein is one such protein that recognizes and binds to the acetylated lysine residues in the histone tail. Inhibition of this protein: protein binding event is of major interest in some forms of cancer. This application highlights an HTS screening assay useful for studying the inhibition of bromodomain/histone binding. Continued interest in histone modifying proteins as druggable targets makes these types of assays an important focus area given the increased performance of the Synergy™ Neo HTS instrument. The assay is an extremely rapid and easy to perform biochemical assay with little optimization required aside from some initial instrumentation optimization. This assay was easily scaled to a 384-well format required for screening of small compound libraries.

Further Information

Related Content

BioTek Introduces New General Manager in Germany
Company appoints Dr. Frank Siefert who will be responsible for managing day-to-day operations in Germany.
Tuesday, November 03, 2015
BioTek to Exhibit at Neuroscience 2015
Join BioTek Instruments as they demonstrate the latest microplate innovations at the SfN’s 45th annual meeting. Neuroscience 2015 will be held October 17-21 at McCormick Place in Chicago, IL. BioTek will showcase their products at booth #1809.
Tuesday, September 29, 2015
BioTek Readers Receive BellBrook Certifications
Company’s multi-mode microplate readers earn Transcreener HTS Assay certifications.
Friday, September 11, 2015
BioTek Readers Receive Promega DLReady™ Certification
Promega’s Dual-Luciferase© Reporter System demonstrate both companies’ common commitment.
Friday, August 07, 2015
BioTek Instruments Receives 5x5x5 Growth Award for Technology
BioTek Instruments has been recognized as one of the 5x5x5 Growth Award winners for 2015.
Wednesday, July 29, 2015
BioTek Expands in the United Kingdom
Company expands their ongoing investment with the opening of their new UK Office in Swindon.
Friday, June 05, 2015
Biotek Named as Finalist for Top Employer Award
The company have been announced as a finalist for the Secretary of Defense Employer Support Freedom Award.
Thursday, May 21, 2015
BioTek Nominated for Department of Defense’s Top Employer Award
BioTek Instruments been nominated for the 2015 Secretary of Defense Employer Support Freedom Award by the Employer Support of the Guard and Reserve (ESGR), a Department of Defense (DoD) office.
Tuesday, March 31, 2015
BioTek Receives 2014 Supplier of the Year Award from Fisher Scientific
BioTek was presented with the award at Thermo Fisher Scientific’s annual meeting in Phoenix.
Wednesday, March 25, 2015
BioTek to Receive 2014 Governor’s Excellence in Worksite Wellness Award
Award recognises worksites in Vermont that promote the health and wellness of their employees.
Wednesday, January 21, 2015
BioTek Receives Two Awards from ESGR
Company has received the Patriot Award and the Above and Beyond Award.
Friday, August 22, 2014
BioTek Receives Extraordinary New Product Line Award from Fisher
Company receives award for Cytation 3 Cell Imaging Multi-Mode Reader.
Tuesday, March 04, 2014
BioTek Announces Establishment of Japanese Office
BioTek Japan will be fully functional by July 1, 2013.
Monday, June 10, 2013
BioTek Responds to Growing AP Market Needs with New Taiwan Office
New office will focus on increasing BioTek's brand presence in Taiwan.
Tuesday, May 14, 2013
BioTek Wins Governor's Award for Outstanding Workplace Safety
BioTek receives the award by Governor Peter Shumlin at the 9th Annual Vermont Workplace Safety Conference.
Thursday, April 18, 2013
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos