Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Natural Protein Points to New Inflammation Treatment
Findings may offer insight to effective treatments for inflammatory diseases, such as rheumatoid arthritis, psoriasis, and multiple sclerosis.
Genetic Cause of Rare Allergy
Institute has identified a genetic mutation responsible for a rare form of inherited hives induced by vibratory urticaria.
Battery Component Found to Harm Key Soil Microorganism
The material at the heart of the lithium ion batteries that power electric vehicles, laptop computers and smartphones has been shown to impair a key soil bacterium, according to new research.
Keeping Tumor Growth at Bay
Engineers at Washington University in St. Louis found a way to keep a cancerous tumor from growing by using nanoparticles of the main ingredient in common antacid tablets.
Natural Protein Points to New Inflammation Treatment
Findings may offer insight to effective treatments for inflammatory diseases, such as rheumatoid arthritis, psoriasis, and multiple sclerosis.
Mitochondria Shown to Trigger Cell Ageing
An international team of scientists has for the first time shown that mitochondria, the batteries of the cells, are essential for ageing.
Cancer Cells Kill Off Healthy Neighbours
Cancer cells create space to grow by killing off surrounding healthy cells, according to UK researchers working with fruit flies.
Validating the Accuracy of CRISPR-Cas9
IBS Researchers create multiplex Digenome-seq to find errors in CRISPR-Cas9 processes.
Cancer Drug Target Visualized at Atomic Resolution
New study using cryo-electron microscopy shows how potential drugs could inhibit cancer.
Genetic Mechanism Behind Cancer-Causing Mutations
Researchers at Indiana University has identified a genetic mechanism that is likely to drive mutations that can lead to cancer.
Scroll Up
Scroll Down

Improved cell viability assay performance using PrestoBlue® Reagent
Bookmark and Share


Tecan Trading AG

Resazurin is a well-established fluorescent dye that is frequently used in various commercially available assay systems. It is a redox indicator that can be added directly to cells in culture. Viable cells convert the dark blue oxidized form of the dye (resazurin) into a red-fluorescent reduced form (resorufin; λEx = 570 nm; λEm = 590 nm). This system is specific for cell viability as non-viable cells rapidly lose metabolic capacity to reduce resazurin, and thus no fluorescent signal is detected. 

Life Technologies offers an enhanced version of the commonly available resazurin-based assays, the PrestoBlue® Cell Viability Reagent. This novel dye offers the same analytical properties (fluorescence excitation and emission wavelength) as common resazurin-based assays (eg. alamarBlue®), but is characterized by a significantly shorter incubation time (minimum 15 mins vs 1 hr), increasing experimental efficiency and performance. 

This application note describes the evaluation of Life Technologies’ PrestoBlue® Cell Viability Reagent using the Infinite M200 PRO and the Infinite M1000 PRO multimode microplate readers. 


Further Information


Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!