Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
The Changing Tides of the In Vitro Diagnostics Market
With the increasing focus in personalized medicine, diagnostics plays a crucial role in patient monitoring.
Immunotherapy Agent Benefits Patients with Drug-Resistant Multiple Myeloma in First Human Trial
Daratumumab proved generally safe in patients, even at the highest doses.
Low-level Arsenic Exposure Before Birth Associated with Early Puberty in Female Mice
Study examine whether low-dose arsenic exposure could have similar health outcomes in humans.
Inciting an Immune Attack On Cancer Cells
A new minimally invasive vaccine that combines cancer cells and immune-enhancing factors could be used clinically to launch a destructive attack on tumors.
‘Mutation-Tracking’ Blood Test for Breast Cancer
Scientists have developed a blood test for breast cancer able to identify which patients will suffer a relapse after treatment, months before tumours are visible on hospital scans.
Cellular Contamination Pathway for Heavy Elements Identified
Berkeley Lab scientists find that an iron-binding protein can transport actinides into cells.
Intensity of Desert Storms May Affect Ocean Phytoplankton
MIT study finds phytoplankton are extremely sensitive to changing levels of desert dust.
Common ‘Heart Attack’ Blood Test May Predict Future Hypertension
Small rises in troponin levels may have value as markers for subclinical heart damage and high blood pressure.
LaVision BioTec Reports on the Neuro Research on the Human Brain After Trauma
Company reports on the work of Dr Ali Ertürk from the Institute for Stroke and Dementia Research at LMU Munich.
NIH Study Shows No Benefit of Omega-3 Supplements for Cognitive Decline
Research was published in the Journal of the American Medical Association.
Scroll Up
Scroll Down

Live Cell Biosensor Assay Used To Interrogate GPCRs - Comparative Analysis of Biosensor Variants
Bookmark and Share


BioTek Instruments

Functional, cell-based assays are essential to identify modulators of GPCRs, including those that signal via increases or decreases in cAMP. Previously, we demonstrated the automation of the GloSensorTM cAMP Assay from Promega, which utilizes a live cell biosensor consisting of a fusion of a cAMP binding domain to a circularly permuted form of firefly luciferase. Upon binding to cAMP, conformational changes in the expressed sensor lead to increases in light output in the presence of substrate. Several variants of the biosensor exist with different affinities for cAMP, some offering increased sensitivity and others offering increased dynamic range. The sensor with the broadest dynamic range, 22F, has the lowest affinity for cAMP, where the basal signal for this construct can be difficult to detect under select conditions. 

This application note compares the performance of the highest and lowest affinity variants using automated methods and the highly-sensitive bottom reading capabilities of an HTS microplate reader.

Further Information

Related Content

BioTek Readers Receive Promega DLReady™ Certification
Promega’s Dual-Luciferase© Reporter System demonstrate both companies’ common commitment.
Friday, August 07, 2015
BioTek Instruments Receives 5x5x5 Growth Award for Technology
BioTek Instruments has been recognized as one of the 5x5x5 Growth Award winners for 2015.
Wednesday, July 29, 2015
BioTek Expands in the United Kingdom
Company expands their ongoing investment with the opening of their new UK Office in Swindon.
Friday, June 05, 2015
Biotek Named as Finalist for Top Employer Award
The company have been announced as a finalist for the Secretary of Defense Employer Support Freedom Award.
Thursday, May 21, 2015
BioTek Nominated for Department of Defense’s Top Employer Award
BioTek Instruments been nominated for the 2015 Secretary of Defense Employer Support Freedom Award by the Employer Support of the Guard and Reserve (ESGR), a Department of Defense (DoD) office.
Tuesday, March 31, 2015
BioTek Receives 2014 Supplier of the Year Award from Fisher Scientific
BioTek was presented with the award at Thermo Fisher Scientific’s annual meeting in Phoenix.
Wednesday, March 25, 2015
BioTek to Receive 2014 Governor’s Excellence in Worksite Wellness Award
Award recognises worksites in Vermont that promote the health and wellness of their employees.
Wednesday, January 21, 2015
BioTek Receives Two Awards from ESGR
Company has received the Patriot Award and the Above and Beyond Award.
Friday, August 22, 2014
BioTek Receives Extraordinary New Product Line Award from Fisher
Company receives award for Cytation 3 Cell Imaging Multi-Mode Reader.
Tuesday, March 04, 2014
BioTek Announces Establishment of Japanese Office
BioTek Japan will be fully functional by July 1, 2013.
Monday, June 10, 2013
BioTek Responds to Growing AP Market Needs with New Taiwan Office
New office will focus on increasing BioTek's brand presence in Taiwan.
Tuesday, May 14, 2013
BioTek Wins Governor's Award for Outstanding Workplace Safety
BioTek receives the award by Governor Peter Shumlin at the 9th Annual Vermont Workplace Safety Conference.
Thursday, April 18, 2013
BioTek Germany Certified in Accordance with EN ISO 13485
Certification guarantees that all of BioTek’s products and services satisfy the requirements and comply with the currently valid legislation.
Monday, December 17, 2012
BioTek Among 5x5x5 Growth Award Special Honorees
Annual award is presented to Vermont companies experiencing outstanding growth in one of five business categories.
Friday, September 21, 2012
BioTek Receives European Patent for Microplate Washer Technology
Ultrasonic Advantage uses unique, integrated ultrasonic cleaning to reduce protein and salt crystal build-up.
Tuesday, June 12, 2012
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!