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Automated Dispensation of Yttrium Oxide SPA Imaging Beads, into 1536-Well Plates Using Deerac Fluidics’ Equator™ HTS Pipetting System
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Deerac Fluidics

Abstract

The drive towards miniaturization within the pharmaceutical field has created a need for liquid handling technologies that accurately deliver low volume reagents to high-density well plates. GE Healthcare’s Scintillation Proximity Assay (SPA) provides an innovative approach for assay  development and biochemical screening that allows the rapid and sensitive measurement of a wide variety of molecular interactions in a homogeneous system.

Here we demonstrate the successful combination of the use of the Equator HTS pipetting system to dispense Streptavidin coupled Yttrium Oxide (YOx) SPA Imaging beads. 2ul of YOx beads were dispensed into1536-well plates containing an excess of 3H-biotin. Plates were read on LEADseeker™ Multimodality Imaging System and analyzed for accuracy and reproducibility. CVs of 6-7% were obtained. Outliers were within spec of 10 or less per 1000 wells.

These results demonstrate the efficacy of the Equator™ HTS with built-in stirred reservoir for the automation of the dispensation of YOx beads to high density plate formats.

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