Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Releasing Cancer Cells for Better Analysis
A new device developed at the University of Michigan could provide a non-invasive way to monitor the progress of an advanced cancer treatment.
Releasing Cancer Cells for Better Analysis
A new device developed at the University of Michigan could provide a non-invasive way to monitor the progress of an advanced cancer treatment.
Apricot Kernels Pose Risk of Cyanide Poisoning
Eating more than three small raw apricot kernels, or less than half of one large kernel, in a serving can exceed safe levels. Toddlers consuming even one small apricot kernel risk being over the safe level.
Cell Transplant Treats Parkinson’s in Mice
A University of Wisconsin—Madison neuroscientist has inserted a genetic switch into nerve cells so a patient can alter their activity by taking designer drugs that would not affect any other cell.
Understanding Female HIV Transmission
Glowing virus maps points of entry through entire female reproductive tract for first time.
Genetic Markers Influence Addiction
Differences in vulnerability to cocaine addiction and relapse linked to both inherited traits and epigenetics, U-M researchers find.
Lab-on-a-Chip for Detecting Glucose
By integrating microfluidic chips with fiber optic biosensors, researchers in China are creating ultrasensitive lab-on-a-chip devices to detect glucose levels.
A lncRNA Regulates Repair of DNA Breaks in Breast Cancer Cells
Findings give "new insight" into biology of tough-to-treat breast cancer.
COPD Linked to Increased Bacterial Invasion
Persistent inflammation in COPD may result from a defect in the immune system that allows airway bacteria to invade deeper into the lung.
Detection of HPV in First-Void Urine
Similar sensitivity of HPV test on first void urine sample compared to cervical smear.
Scroll Up
Scroll Down

Efficient PCR from whole blood using Finnzymes High Performance PCR solution
Bookmark and Share


Labtech International

Finnzymes have developed a range of reagents,consumables and instrumentation to allow fastPCR in inhibiting conditions. Here we describean application for amplifying exogenous andhuman genomic DNA from whole blood withoutthe need for DNA purification, and use ofspecial buffers or complicated protocols.Finnzymes' solution for High Performance (HP)-PCR results in faster amplification with betteryields for raw blood samples as compared withconventional methods for PCR.

Finnzymes’ HP-PCR is a tripartite solution focused onspeeding up a PCR and providing marked improvementsin yield while maintaining high fidelity. The uniquecombination of our highly processive PhusionTM DNAPolymerases, quick PikoTM Thermal Cyclers and ultra-thinwalled UTWTM tubes provides considerable benefits to thespeed, yield, fidelity, specificity and robustness of PCR.Central to these improvements is our Phusion technology,which enhances activity by way of a double-strandedDNA-binding domain bound to a highly engineeredproofreading polymerase. This dsDNA-binding domainallows for ultra-robust reactions by tethering thepolymerase to the duplex DNA, driving much higherassociation constants, so the effects of conventionalinhibitors of PCR are significantly reduced1. Here wedescribe one such application, a simple protocol for robustamplification of DNA from whole blood. Whole blood isoften used in clinical diagnostics and as a DNA source forPCR tests. Unfortunately, Taq DNA polymerase, the mostcommonly used enzyme for PCR, is known to becompletely inhibited by even small quantities of wholeblood in the reaction2. Several strategies have been usedto overcome this difficulty, including the use of specializedpurifications, buffers and pretreatments3,4, but all of thesemethods have drawbacks, adding time and cost, and oftenproducing inconsistent results. As a consequence,purification of genomic DNA from whole blood remains acommon practice even though it adds considerable timeand expense to the test.

Further Information


Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,400+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!