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Achieving Efficient Delivery of Morpholino Oligos with Nucleofection®
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Amaxa GmbH

Morpholino oligos provide a stable, specific and effective antisense activity. Developmental biologists have long since used these properties by microinjecting or electroporating Morpholinos into embryos to allow for splice-blocking or gene knockdown. However, efficient delivery of Morpholinos in mammalian cell cultures can be a challenge. While complex formation with lipid-based reagents is ineffective due to the non-ionic Morpholino backbone, we show here that Nucleofection® can be used as an efficient delivery method.

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