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Nucleofection® of mouse ES cells
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Amaxa GMBH

Efficient delivery of DNA is crucial for gene targeting in embryonic stem cells and for successful generation of knock-out mice. Data presented in this technote show that Nucleofection® leads to a homogenous gene expression pattern in mouse ES cell colonies. In contrast to standard electroporation, Nucleofection® results in a 100% deletion of frt-flanked genes on both alleles. Viable and fertile mice were generated from transfected mouse ES cell clones.

Further Information

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Amaxa to Host Webinar on the Nucleofection® of Stem Cells
The webinar imparts knowledge about setting up embryonic, mesenchymal, hematopoietic or neural stem cell experiments using Nucleofector® Technology.
Thursday, May 01, 2008
amaxa to Co-ordinate Collaboration to Develop High Throughput Devices for Primary Cell Transfection
amaxa receives a €2.75M grant from European Commission to develop devices for use in the study of immunological, neuronal and liver disorders.
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