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Sorting Through Cellular Statistics
Aaron Dinner, professor in chemistry, and his graduate student Herman Gudjonson are trying to read the manual of life, DNA, as part of the Dinner group’s research into bioinformatics—the application of statistics to biological research.
Playing 'Tag' with Pollution lets Scientists See Who's It
Using a climate model that can tag sources of soot from different global regions and can track where it lands on the Tibetan Plateau, researchers have determined which areas around the plateau contribute the most soot — and where.
Women’s Immune System Genes Operate Differently from Men’s
A new technology reveals that immune system genes switch on and off differently in women and men, and the source of that variation is not primarily in the DNA.
Long Telomeres Associated with Increased Lung Cancer Risk
Genetic predisposition for long telomeres predicts increased lung adenocarcinoma risk.
First Artificial Ribosome Designed
Researchers at the University of Illinois at Chicago and Northwestern University have engineered a tethered ribosome that works nearly as well as the authentic cellular component, or organelle, that produces all the proteins and enzymes within the cell.
High-Resolution 3D Images Reveal the Muscle Mitochondrial Power Grid
NIH mouse study overturns scientific ideas on energy distribution in muscle.
Expanding the Brain
A team of researchers has identified more than 40 new “imprinted” genes, in which either the maternal or paternal copy of a gene is expressed while the other is silenced.
Identifying a Key Growth Factor in Cell Proliferation
Researchers discover that aspartate is a limiter of cell proliferation.
Study Uncovers Target for Preventing Huntington’s Disease
Scientists from Cardiff University believe that a treatment to prevent or delay the symptoms of Huntington’s disease could now be much closer, following a major breakthrough.
Paving the way to Better Ovarian Cancer Diagnosis
Aïcha BenTaieb will present her invention for automated identification of ovarian cancer’s many subtypes at an international conference this fall.
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Next-generation sequencing of highly multiplexed samples: the NuGEN Encore™ 384 Multiplex System
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NuGEN Technologies, Inc.

Advances in DNA sequencing throughput afforded by highly parallel, reversible-terminator sequencing platforms have completely redefined our view of biological inquiry. Crisp, high-resolution, digital views of whole-genome biology provide both profound clarity and significance. As with any disruptive technology, implementation creates turbulence. In the case of NGS, accelerated sequencing throughput has created challenges in upstream sample preparation and downstream data analysis. Initially, sample preparation was particularly problematic because it required individuals with highly specialized molecular biology skills for the hands-on execution of complex protocols. Fortunately, automated solutions for NGS library preparation have arrived, and it is reasonable to expect broad dissemination of turnkey solutions for routine library generation in the very near future. What remains problematic is that some sequencing projects involve large numbers of samples that require relatively shallow sequencing coverage. The sequencing costs of such projects are often prohibitive, calling for methods to multiplex high sample numbers in the same sequencing run. The obvious solution is to combine many samples into a single sequencing lane, and there are now many reports in the literature describing ad hoc methods and strategies for multiplexing samples prior to sequencing. Here we introduce a commercial solution for deep multiplexing of NGS samples that builds on our Encore™ NGS Multiplex System. The Encore 384 Multiplex System consists of a refined set of 384 molecularly “bar-coded” library adaptors that enable deep multiplexing of sequencing samples within a library preparation system that is compatible with high-throughput automation. The version of the product discussed in this White Paper contains adaptor sequences compatible with Illumina sequencing platforms.

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