Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Resources>White Papers>This White Paper
  White Papers
Scientific News
Big Genetics in BC: The American Society for Human Genetics 2016 Meeting
Themes at this year's meeting ranged from the verification, validation, and sharing of data, to the translation of laboratory findings into actionable clinical results.
Stem Cells in Drug Discovery
Potential Source of Unlimited Human Test Cells, but Roadblocks Remain.
Automated Low Volume Dispensing Trends
Gain a better understanding of the current and future market requirements for fully automated LVD systems.
Personality Traits, Psychiatric Disorders Linked to Specific Genomic Locations
Researchers have unearthed genetic correlations between personality traits and psychiatric disorders.
Forensic 3D Documentation of Skin Injuries
In this study, the validity of using photogrammetry for documenting injuries in a pathological context was demonstrated.
3-D Printed Dog’s Nose Improves Vapor Detection
By mimicking how dogs get their whiffs, a team of government and university researchers have demonstrated that “active sniffing” can improve by more than 10 times the performance of current technologies that rely on continuous suction to detect trace amounts of explosives and other contraband.
New Markers for Forensic Body-fluid Identification
University of Bonn researchers have successfully identified specific Micro-RNA signatures to help forensically identify body fluids.
Genetics Control Regenerative Properties Of Stem Cells
Researchers define how genetic factors control regenerative properties of blood-forming stem cells.
Major Neuroscience Initiative Launched
Tianqiao and Chrissy Chen Institute invest $115 million to further expand neuroscience research, while Caltech construct $200 million biosciences complex.
Making It Personal
Cancer vaccine linked to increased immune response against leukemia cells.
Scroll Up
Scroll Down

Next-generation sequencing of highly multiplexed samples: the NuGEN Encore™ 384 Multiplex System
Bookmark and Share


NuGEN Technologies, Inc.

Advances in DNA sequencing throughput afforded by highly parallel, reversible-terminator sequencing platforms have completely redefined our view of biological inquiry. Crisp, high-resolution, digital views of whole-genome biology provide both profound clarity and significance. As with any disruptive technology, implementation creates turbulence. In the case of NGS, accelerated sequencing throughput has created challenges in upstream sample preparation and downstream data analysis. Initially, sample preparation was particularly problematic because it required individuals with highly specialized molecular biology skills for the hands-on execution of complex protocols. Fortunately, automated solutions for NGS library preparation have arrived, and it is reasonable to expect broad dissemination of turnkey solutions for routine library generation in the very near future. What remains problematic is that some sequencing projects involve large numbers of samples that require relatively shallow sequencing coverage. The sequencing costs of such projects are often prohibitive, calling for methods to multiplex high sample numbers in the same sequencing run. The obvious solution is to combine many samples into a single sequencing lane, and there are now many reports in the literature describing ad hoc methods and strategies for multiplexing samples prior to sequencing. Here we introduce a commercial solution for deep multiplexing of NGS samples that builds on our Encore™ NGS Multiplex System. The Encore 384 Multiplex System consists of a refined set of 384 molecularly “bar-coded” library adaptors that enable deep multiplexing of sequencing samples within a library preparation system that is compatible with high-throughput automation. The version of the product discussed in this White Paper contains adaptor sequences compatible with Illumina sequencing platforms.

Further Information


Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
4,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,300+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!