Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Resources>White Papers>This White Paper
  White Papers
Scientific News
Big Genetics in BC: The American Society for Human Genetics 2016 Meeting
Themes at this year's meeting ranged from the verification, validation, and sharing of data, to the translation of laboratory findings into actionable clinical results.
Stem Cells in Drug Discovery
Potential Source of Unlimited Human Test Cells, but Roadblocks Remain.
Cancer Genetics: Key to Diagnosis, Therapy
When applied judiciously, cancer genetics directs caregivers to the right drug at the right time, while sparing patients of unnecessary or harmful treatments.
Transporting Microscopic Cargo Between Human Cells
Scientists have developed a virus-inspired delivery system for material transport between cells.
Metabolite Promotes Cancer Cell Transformation
Researchers have identified a metabolite that promotes cancer cell transformation and colorectal cancer spread.
Improving Drug Production with Computer Model
A model has been developed that can be used to improve and accelerate the production of biotherapeutics, cancer drugs, and vaccines.
Zika’s Entry Points
Discovery shows Zika infection of neural progenitor cells occurs regardless of AXL production, which was thought to be the main vector for the virus.
New Form of Autism Found
An international team of researchers have identified a new form of syndromic autism.
Radiation-Free Imaging in the Brain
Scientists create sensors that use proteins to detect particular targets through induced blood flow changes.
Failings in Conveying Risks of Undercooked Meat
A study has found that restaurants do not communicate the risks of eating undercooked meats.
Scroll Up
Scroll Down

A Review of Imaging Techniques Compatible with Three Dimensional Culture of Cells Grown in Alvetex® Scaffold
Bookmark and Share


Reinnervate

As scientists better understand the benefits of growing cells in three dimensions (3D) and routinely adopt 3D culture techniques, methods for visualising cells must also be adapted and optimised.

The most common and routinely used technique for tracking two dimensional (2D) cell cultures is light microscopy. Traditional 2D monolayer cultures are highly transparent and within a single optical plane. The minimal light diffraction and diffusion presented by the plastic surface allows the collection of focussed microscopic images. Cells cultured in genuine 3D environments, such as in Alvetex® Scaffold present some of the same constraints as tissue samples or biopsies in that simple, live observation of cultures via phase microscopy is not optimal. There are however, other techniques that can be implemented which will allow the user to monitor culture progress easily and effectively in 3D; Simple dyes can be used to identify culture confluence and viability. The varietyof end-point visualisation techniques available to those culturing cells in 3D is extensive. Options include, but arenot limited to, live cell imaging, fluorescent marker analysis, confocal analysis, histology using a range of cytological stains and electron microscopy. All of these techniques have been performed on cultures grown in Alvetex® Scaffold with excellent results. Here we review common imaging methods and outline their use and suitability for cultures grown in 3Dwithin the Alvetex® Scaffold.

Further Information

Related Content

Reinnervate Partners with Oncotest and SBH Sciences
Companies to partner on introductory services for screening novel anti-cancer drugs using 3D tumour cell culture.
Thursday, October 31, 2013
Reinnervate Ltd and Roslin Cellab Partner
Reinnervate Ltd announced that it is entering into a collaboration with Roslin Cellab to develop 3D stem cell printing products and services using Alvetex® technology.
Friday, September 20, 2013
3D cell Culture Firm Reinnervate and Specialist Oncology CRO Oncotest GmbH Enter Collaboration Agreement
Oncotest to offer enhanced pre-clinical oncology and cancer research screening services incorporating Alvetex®Scaffold 3D cell culture technology.
Monday, January 07, 2013
Reinnervate and Tecan Sign Co-marketing Agreement
Both Companies to develop automated 3D cell culture solutions for improved cellular assays.
Tuesday, November 27, 2012
Reinnervate and Roslin Cellab Announce Plans to Collaborate
Collaboration on the development of protocols for three dimensional growth of hESC’s using Alvetex®Scaffold.
Friday, April 27, 2012
Reinnervate and Kirkstall to Collaborate on New 3D Cell Culture Products
Companies will collaborate to bring next generation three dimensional (3D) cell culture products to market in 2012. The two UK companies develop cell culture systems that bridge the current gap between 2D in-vitro cell culture and animal models.
Monday, March 19, 2012
New Reinnervate Whitepaper Includes Details on Live 3D Cell Imaging in 3D Cell Culture Using Alvetex®
The release is set to help scientists make the switch from traditional 2D cell culture to 3D cell growth.
Monday, January 30, 2012
Reinnervate and Mirus Bio announce collaboration
Reinnervate and Mirus Bio announce commercial agreement to launch new range of products for the transfection of cells cultured in 3D.
Friday, November 18, 2011
Alvetex® Voted One of Most Technologically Significant New Research Products By R&D Magazine
Alvetex® enables genuine 3D cell growth to be performed routinely and cost effectively in cell biology laboratories.
Monday, June 27, 2011
Reinnervate Partners with AMS Biotechnology (Europe) Limited for Distribution of Alvetex®
Alvetex® is a unique and proprietary cell culture scaffold that enables genuine 3D cell growth to be performed routinely and cost-effectively in cell biology laboratories.
Wednesday, June 01, 2011
Reinnervate Enhances Commercial and Product Development Teams with Key Senior Appointments
Appointments of Richard Rowling as commercial director and Barry Lynch as marketing manager to lead the sales and marketing operations supporting alvetex®.
Wednesday, April 06, 2011
Alvetex® Named in the “Top Ten Life Science Innovations of 2010” by The Scientist Magazine
Reinnervate’s 3D cell culture technology selected as one the ten most exciting new tools to hit the life sciences market this year.
Monday, December 06, 2010
Reinnervate Partners with LGC for Global Distribution of Alvetex®
Additional collaboration to develop 3d cell cultures for applications in drug discovery and development.
Tuesday, November 30, 2010
Reinnervate Partners with LGC for Global Distribution of ALVETEX®
Additional collaboration to develop 3D cell cultures for applications in drug discovery and development.
Monday, November 29, 2010
Reinnervate Completes Expansion into New Purpose-Built Facility at Netpark
Reinnervate, a life sciences company driving the adoption of routine 3D cell culture, has announced its expansion into a new purpose-built research and production facility at NETPark near Sedgefield, UK.
Tuesday, November 16, 2010

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,300+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!