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Improved Cryopreservation of Human Hepatocytes

Published: Tuesday, June 26, 2012
Last Updated: Tuesday, June 26, 2012
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Cryopreservation of hepatocytes by using STEM-CELLBANKER® cryopreservation solution from AMSBIO.

A recently published article from researchers at the Karolinska Institute details an improved protocol for cryopreservation of primary human hepatocyte using STEM-CELLBANKER® cryopreservation solution from AMSBIO.

In the article the authors compare cryopreservation of liver hepatocytes using STEM-CELLBANKER with the frequently used DMSO-University of Wisconsin (DMSO-UW) medium protocol.

Both protocols yielded hepatocytes with good P450 function, however, the STEM-CELLBANKER protocol gave a higher viability than the DMSO-UW cryoprotectant.

The researchers concluded that STEM-CELLBANKER beneficially is also xeno-free and will be applicable to cryopreservation of clinical-grade primary human hepatocytes.

STEM-CELLBANKER from AMSBIO is a chemically defined freezing medium with optimized formulation for stem cells and iPS cells storage as well as other valuable cells.

Supplied ready-to-use with a simple usage protocol, STEM-CELLBANKER is completely free of serum and animal derived components, and contains only European or US Pharmacopoeia graded ingredients.

Part of the CELLBANKER® series of cell freezing media, STEM-CELLBANKER® significantly increases cell viability while maintaining cell pluripotency, normal karyotype and proliferation ability following resuscitation from cryopreservation, even after extended long-term storage.

Every batch of STEM-CELLBANKER® cryopreservation medium is performance tested on Jurkat and SK-007 cells.

Additional standard evaluations for endotoxins, pH, osmolarity and Mycoplasma contaminants are undertaken to ensure GMP equivalent quality.

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