Corporate Banner
Satellite Banner
Stem Cells, Cellular Therapy & Biobanking
>
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Stem Cells may do Best with a Little Help from their Friends

Published: Wednesday, September 11, 2013
Last Updated: Wednesday, September 11, 2013
Bookmark and Share
“Helper cells” improve survival rate of transplanted stem cells, mouse study finds.

Like volunteers handing out cups of energy drinks to marathon runners, specially engineered “helper cells” transplanted along with stem cells can dole out growth factors to increase the stem cells’ endurance, at least briefly, Johns Hopkins researchers report. Their study, published in the September issue of Experimental Neurology, is believed to be the first to test the helper-cell tactic, which they hope will someday help to overcome a major barrier to successful stem cell transplants.

“One of the bottlenecks with stem cell therapy is the survival of cells once they’re put in the body — about 80 to 90 percent of them often appear to die,” says Jeff Bulte, Ph.D., a professor in the Johns Hopkins University School of Medicine’s Institute for Cell Engineering. “We discovered it helps to put the stem cells in with some buddies that give off growth factors.”

Stem cells can morph to take on any role in the body, making them theoretically useful to treat conditions ranging from type 1 diabetes (replacing insulin-producing cells in the pancreas) to heart disease (taking over for damaged heart cells). The biggest problem for transplanted stem cells, Bulte says, is that they’re initially grown in a dish with ready access to oxygen, then put in the body, where levels are relatively low. “They get a shock,” he says. Other research groups have had some success with acclimating cells to lower oxygen levels before transplantation; another promising strategy has been to provide the stem cells with scaffolds that give them structure and help integrate them with the host.

The research team, spearheaded by postdoctoral fellow Yajie Liang, Ph.D., wondered whether the cells’ survival could also be enhanced with steady doses of a compound called basic fibroblast growth factor (bFGF), an “energy drink” that spurs cells to grow. They engineered cultured human and mouse cells to make greater-than-normal amounts of bFGF under the control of a drug called doxycycline (dox). Making the bFGF gene responsive to dox meant the researchers could control how much bFGF was made, Liang explains.

The team then transplanted the engineered helper cells and stem cells into mice. The stem cells had themselves been engineered to make a luminescent protein, and using a special optical instrument, the researchers could monitor the intensity of the luminescence through the animals’ skin to see how many of the cells were still alive. The team gave the mice steady doses of dox to keep the bFGF flowing.

For the first three days after injection, the stem cells with helpers gave off a noticeably stronger signal than stem cells transplanted alone, Liang says, but a few days later, there was no difference between the two.

Despite the short duration of the helper cells’ effect, Bulte says, the experiment shows the potential of using helper cells in this way. Perhaps the ultimate solution to keeping transplanted stem cells alive will be to use helpers that give off a cocktail of growth factors, he suggests, as well as pre-conditioning for low oxygen conditions and scaffolds. “Once the rubber hits the road, it’s very important that the stem cells survive for a long time,” he says.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,900+ scientific posters on ePosters
  • More than 4,200+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Bad Luck of Random Mutations Plays Predominant Role in Cancer, Study Shows
Statistical modeling links cancer risk with number of stem cell divisions.
Tuesday, January 06, 2015
Researchers Use Human Stem Cells to Create Light-Sensitive Retina in a Dish
Johns Hopkins researchers have created a 3-D complement of human retinal tissue in the laboratory.
Saturday, June 14, 2014
Signals Found That Recruit Host Animals’ Cells, Enabling Breast Cancer Metastasis
Mouse studies suggest that blocking aid from white blood cells and stem cells could keep tumors contained.
Thursday, May 22, 2014
Tumor-suppressor Protein Gives Up Its Secrets
Discovery promises new targets for cancer drug design.
Friday, July 12, 2013
Patients’ Skin Cells are Transformed into Heart Cells to Create a ‘Disease-In-A-Dish’
Lab model of an inherited, life-threatening disease known as ARVD/C allows researchers to study the condition and find ways to treat it.
Monday, February 18, 2013
Scientific News
Common Cell Transformed into Master Heart Cell
By genetically reprogramming the most common type of cell in mammalian connective tissue, researchers at the University of Wisconsin—Madison have generated master heart cells — primitive progenitors that form the developing heart.
Improving Regenerative Medicine
Lab-created stem cells may lack key characteristics, UCLA research finds.
Muscles on-a-Chip
This study may help explain why stem cell-based therapies have so far shown limited benefits for heart attack patients in clinical trials.
3-D Printed Lifelike Liver Tissue for Drug Screening
A team led by engineers at the University of California, San Diego has 3D-printed a tissue that closely mimics the human liver's sophisticated structure and function. The new model could be used for patient-specific drug screening and disease modeling.
Therapeutic Approach Gives Hope for Multiple Myeloma
A new therapeutic approach tested by a team from Maisonneuve-Rosemont Hospital (CIUSSS-EST, Montreal) and the University of Montreal gives promising results for the treatment of multiple myeloma, a cancer of the bone marrow currently considered incurable with conventional chemotherapy and for which the average life expectancy is about 6 or 7 years.
Cat Stem Cell Therapy Gives Humans Hope
By the time Bob the cat came to the UC Davis veterinary hospital, he had used up most of his nine lives.
Bile Acid Supports Production of Blood Stem Cells
A research group at Lund University has been able to show that bile acid is transferred from the mother to the foetus via the placenta to enable the foetus to produce blood stem cells.
New Biomarker to Assess Stem Cells Developed
A research team led by scientists from UCL have found a way to assess the viability of 'manufactured' stem cells known as induced pluripotent stem cells (iPSCs). The team's discovery offers a new way to fast-track screening methods used in stem cell research.
Tricked-Out Immune Cells Could Attack Cancer
New cell-engineering technique may lead to precision immunotherapies.
Edited Stem Cells Offer Hope of Precision Therapy for Blindness
Findings raise the possibility of treating blinding eye diseases using a patient's own corrected cells as replacement tissue.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!