Corporate Banner
Satellite Banner
Stem Cells, Cellular Therapy & Biobanking
>
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Stem Cells Form Light-Sensitive 3-D Retinal Tissue

Published: Tuesday, June 24, 2014
Last Updated: Tuesday, June 24, 2014
Bookmark and Share
Researchers induced human stem cells to create a 3-D retina structure that responds to light. The finding may aid the study of eye diseases and could eventually lead to new therapies.

When we view a sunset or a soccer game, an intricate, orchestrated series of events takes place in our eyes. Light passes through the front part of each eye and is refracted and focused on the retina, a thin, delicate tissue at the back of our eyes. The retina contains many specialized cells, including photoreceptor cells, which convert light into electrical signals. These signals are then processed and sent to the brain. If the photoreceptor cells malfunction or die, vision loss and blindness can occur.

Researchers have previously shown that induced pluripotent stem (iPS) cells can grow into a type of retinal cell under certain cell culture conditions. These types of stem cells are adult cells that have been genetically reprogrammed to take on the characteristics of embryonic stem cells. They can grow indefinitely in the laboratory and can theoretically change, or differentiate, into all cell types found in the body.

Previous work showed that mouse and human embryonic stem cells can develop into a 3-D optic cup in culture that resembles the embryonic vertebrate eye. A team led by Drs. Xiufeng Zhong and M. Valeria Canto-Soler at Johns Hopkins University set out to determine to what extent iPS cells could be prompted to differentiate and acquire structural and functional features similar to a human retina. The work was funded in part by NIH’s National Eye Institute (NEI) and National Heart, Lung, and Blood Institute (NHLBI).

The scientists grew human iPS cells in culture dishes in the lab. They reported online on June 10, 2014, in Nature Communications that they were able to coax the cells to gradually take on the characteristics of retinal cells without adding many of the chemicals typically used to induce the cells to mature.

Over time, the iPS cells spontaneously formed cup-like 3-D structures in a sequence of events that mimicked what occurs during human development. The structures developed layers containing all the major cell types that are normally present in the retina, including photoreceptor cells.

To determine whether the photoreceptor cells were sensitive to light, the researchers subjected cells to a flash of light and measured the electrical responses in individual cells. A few cells responded, indicating they had reached a fairly advanced stage of development under the culture conditions.

“We have basically created a miniature human retina in a dish that not only has the architectural organization of the retina but also has the ability to sense light,” Canto-Soler says. “When we began this work, we didn't think stem cells would be able to build up a retina almost on their own. In our system, somehow the cells knew what to do.”

The accomplishment may lead to better tissue culture models to study human eye diseases and explore new therapies.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,300+ scientific posters on ePosters
  • More than 4,900+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Stem Cell Therapy Heals Injured Mouse Brain
A team of researchers has developed a therapeutic technique that dramatically increases the production of nerve cells in mice with stroke-induced brain damage.
Tuesday, August 23, 2016
Stem Cells Grown On Scaffold Mimic Hip Joint Cartilage
Adult fat-derived stem cells grown on a 3-D scaffold that mimicked a hip joint surface formed cartilage and maintained the correct shape.
Wednesday, August 10, 2016
Using Animal Embryos Containing Human Cells
With recent advances in stem cell and gene editing technologies, an increasing number of researchers are interested in growing human tissues and organs in animals by introducing pluripotent human cells into early animal embryos.
Monday, August 08, 2016
Treatment Advancement for Gaucher and Parkinson's Diseases
NIH scientists identify molecule that may act as a possible treatment of neurological diseases.
Wednesday, July 13, 2016
NIH Funds Million-Person Medicine Study
NIH announces $55million in awards to build foundations for ambitious Cohort Program that aims to engage 1 million participants in lifestyle, environments and genetics research.
Friday, July 08, 2016
Manufactured Stem Cells To Advance Clinical Research
Clinical-grade cell line will enable development of new therapies and accelerate early-stage clinical research.
Tuesday, June 28, 2016
NIH Funds Biobank To Support Precision Medicine Initiative Cohort Program
$142 million over five years will be awarded to the Mayo Clinic to establish the world’s largest research-cohort biobank for the PMI Cohort Program
Friday, May 27, 2016
Molecule Proves Key to Brain Repair After Stroke
Scientists found that a molecule known as growth and differentiation factor 10 (GDF10) plays a key role in repair mechanisms following stroke.
Tuesday, November 10, 2015
A Patient’s Budding Cortex — In A Dish?
Networking neurons thrive in 3-D human “organoid”
Friday, May 29, 2015
Drugs that Activate Brain Stem Cells May Reverse Multiple Sclerosis
NIH-funded study identifies over-the-counter compounds that may replace damaged cells.
Tuesday, April 21, 2015
Stem Cell Transplants May Halt Progression of Multiple Sclerosis
NIH-funded study yields encouraging early results.
Tuesday, December 30, 2014
Scientists Sniff Out Unexpected Role for Stem Cells in the Brain
NIH scientists find that restocking new cells in the brain’s center for smell maintains crucial circuitry.
Saturday, October 11, 2014
Suspect Gene Corrupts Neural Connections
“Diseases of synapses” demo’d in a dish - NIH-funded study.
Tuesday, August 19, 2014
Early Treatment Benefits Infants with Severe Combined Immunodeficiency
NIH-funded study identifies factors contributing to successful stem cell transplants.
Friday, August 01, 2014
Stem Cell Therapy Rebuilds Heart Muscle in Primates
Human embryonic stem cells used to regenerate damaged primate hearts.
Tuesday, May 13, 2014
Scientific News
Controlling DNA Repair
Scientists discover that DNA repair outcomes following CRISPR-Cas9 cleaving are non-random and can be harnessed to produce desired effects.
Gene Therapy Via Ultrasound
Research into a gene therapy approach called sonoporation could help combat heart disease and cancer.
Stem Cell Therapy Heals Injured Mouse Brain
A team of researchers has developed a therapeutic technique that dramatically increases the production of nerve cells in mice with stroke-induced brain damage.
Challenging Stem Cell Fate Control
Researchers have found that the fate of stem cells is not only controlled by their local niche, but also by a cell-intrinsic mechanism.
Zika Proteins Responsible for Microcephaly Identified
Researchers have undertaken the first study to examine Zika infection in human neural stem cells from second-trimester fetuses.
Heart Muscle from Stem Cells Aid Cardiovascular Medicine
Researchers discover heart muscle cells from stem cells mirror expression patterns of key genes in donor tissue.
Examining New Hypotheses for Undiagnosed Patients
UnDx Consortium gathers in San Diego to create new paths to identifying currently undiagnosed illnesses.
Novel Therapeutic Approach for Blood Disorders
Gene editing of human blood-forming stem cells mimics a benign genetic condition that helps to overcome sickle cell disease and other blood disorders.
Bone Marrow Transplants Without Using Chemotherapy
Scientists have devised a way to destroy blood stem cells in mice without using chemotherapy or radiotherapy, both of which have toxic side effects.
How Cloud Connectivity Can Combat the Reproducibility Crisis
This infographic explains the reproducibility crisis, and how cloud connectivity can help overcome this problem.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,900+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!