|Non-disruptively Count and Quantify Fluorescent iPS Colonies During Secondary Reprogramming: 7 min per 6-well Plate, Dual-fluorescence Whole Well Imaging Cytometry|
SC Cribbes, S Brightwell, K Kaji
Nexcelom’s Celigo imaging cytometer has been applied to provide automated, rapid assessment of iPS reprogramming.
|Human Cardiomyocytes Derived from Induced Pluripotent Stem Cells: High Throughput and High Content Assessment of Cardiac Toxicity and Drug Efficacy by Monitoring Cytosolic Free Calcium Transients|
1Kettenhofen R, 1Duenbostell A, 2Niedereichholz T, 3D’Angelo JM, 4Horai H, 5Schwengberg S, 1Bohlen H, 6Licher T"
Introduction of selected, pure human cardiomyocytes derived from induced pluripotent stem cell (hiPSCM) into a calcium transient imaging high throughput screening (HTS) assay to assess cardiotoxicity and drug efficacies.
|Characterisation & Potential Applications of Human iPS Cell Derived Neural Progenitor Cells |
Wei J1., Gibbons G1., Lopez Alcantara S2., Dale T2., González Rueda A3., Paulsen O3. & Cox C1.
We characterised iPS cell-derived human neural progenitor cells (hNPCs) and their progeny produced using optimised methods to examine their suitability for neurobiology research.
|Genetic integrity of rice seeds during ageing and gene bank storage by SSR analysis|
I. O. Daniel, K. A. Adeboye, O. W. Adabale, E. S. Aladele and A. Boerner
Rice seeds loose germination capacity with ageing. Loses in genetic integrity was found to occur during artificial ageing at a time when percentage germination declined to 54%.
|Rapid analysis of 3D tumour spheroids in soft agar and on ultra-low attachment plates using a laser scanning imaging system|
Anne F Hammerstein, Diana Caracino, and Paul Wylie
The requirement for better in-vitro models that are compatible with high-throughput screening campaigns has led to the development of 3D cell cultures models, especially muliticellular spheroids, which retain many of the morphological and genetic traits of tumours.
Here we describe the formation of such spheroids by two methods: on ultra-low attachment plates and in semi-solid agarose. Both methods are compatible with 96- and 384-well microplate formats.
|Integrating compound storage into automated laboratory workflows |
James Craven, Simon Tullett
This poster discusses the benefits of comPOUND for automated sample storage and delivery. TTP Labtech’s pneumatic transport technology, lab2lab, integrates these stores with other instrumentation into managed, fully automated workflows, allowing the scientist to focus on research and data analysis.
|Chia-Hsun Hsieh and Sanger Hung-Chih Lin win at CellTech |
Sanger Hung-Chih Lin, Chia-Hsun Hsieh, Hung-Chih Hsu, Hung-Ming Wang, Chi-Ya Huang, Min-Hsien Wu and Ching-Ping Tseng
Chia-Hsun Hsieh and Sanger Hung-Chih Lin win at CellTech
|Low cost, low footprint, expandable automated biobanking solutions |
James Craven, Chris Morris, Maud Godfrey, Danielle Miller
This case study describes how TTP Labtech’s comPOUND storage modules have been employed by Abcam, a worldwide supplier of antibodies. Here, the turnaround time of sample placement and retrieval is an essential component for high quality service to its customers.
|Gene Expression from Pseudourine and 5-Methylcytidine Modified Messenger RNA|
Jiehua Zhou, Maggie L. Bobbin, Julie R. Escamilla-Powers, Anton P. McCaffrey and John J. Rossi
Study objective was to develop methodologies for gram scale synthesis of messenger RNA (mRNA) for gene therapy applications, as well as scalable purification methods that yield highly expressed, persistent and non-toxic mRNA.
|ISMTR-283 Protects Human Bone Marrow Progenitors from the Lethal Effects of Ionizing Radiation|
Clarke E, Traynor-Kaplan A
Bone Marrow (BM) is extremely susceptible to radiation damage. BM from matched donors is often used to ‘rescue’ patients following medical radiation for leukemia. However, in the event of accidental/environmental radiation exposure, the ability to identify appropriate donors in a timely manner is rarely possible. Using CFC assays, we evaluated a small molecule compound (ISMTR-283) for its potential to protect the marrow progenitor cells and microenvironment from radiation damage.
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