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Expression of Pluripotency-determining Factors in in vitro Fertilized Buffalo Embryos and Embryonic Stem Cells
T Anand, D Kumar, M S Chauhan, and P Palta

The POU octamer-binding domain transcription factor Oct-4, Stage-specific embryonic antigens (SSEAs), and Tumor rejection antigens (TRAs), are developmentally regulated during early embryogenesis.

Effect of Culture Media and Serum Supplementation on the Development of in vitro Fertilized Buffalo Embryos
D Kumar, T Anand, P Palta and M.S. Chauhan

This poster compares the development of buffalo embryos in simple and complex culture media and aims to determine the effects of serum supplementation on the development of buffalo embryos.

High Content Analysis of Neural Stem Cell Expansion and Differentiation
Oksana Sirenko, Allan C. Powe, Steven L. Stice, Karen Cook, Nick Callamaras, Jayne Hesley, Xin Jiang and Evan F. Cromwell

Automated assay methods for monitoring neural stem cell expansion and differentiation using stem cell derived neural cell lines and high content imaging systems have been described.

Neurotoxicity Assays Using iPSC-Derived Neurons and High Content Imaging
Oksana Sirenko, Susan DeLaura, Lucas Chase, Jayne Hesley and Evan F. Cromwell

Neurotoxicity can cause temporary or permanent damage of brain or peripheral nervous system and has been found to be a major cause of neurodegenerative diseases such as Alzheimer’s or Parkinson’s. Accordingly, there is a great interest in developing more predictive, disease relevant cell-based models and efficient screening tools for assessing the neurotoxicity of chemical compounds, drug candidates and environmental agents.

Live Cell Beating Assay Using Human iPSC-derived Cardiomyocytes for Evaluation of Drug Efficacy and Toxicity
Oksana Sirenko, Carole Crittenden, Blake Anson, Jayne Hesley, Yen-Wen Chen, Nick Callamaras and Evan F. Cromwell

A large percentage of new drugs fail in clinical studies due to cardiac toxicity. Development of highly predictive in vitro assays suitable for screening, safety assessment or other environments is therefore extremely important for drug development. Human cardiomyocytes derived from stem cell sources can greatly accelerate the discovery of cardiac drugs and improve drug safety by offering more clinically relevant cell-based models than those presently available.

Regulation of shoot apical meristem development by SEUSS and SEUSS-LIKE 2 in Arabidopsis
Joanne E. Lee and John F. Golz

In Arabidopsis, SEU and SLK2 are redundant components of a regulatory complex that is proposed to promote shoot apical meristem (SAM) formation during embryogenesis. Expression analysis indicates that SEU and SLK2 act upstream of several known SAM regulators, and also regulate auxin accumulation, probably via interaction with auxin response factors.

Expansion of mesenchymal stem cells from frozen UCB
Christophe NP Madsen and Christian Clausen

Umbilical cord blood (UCB) has recently been the focus of clinical applications. UCB contains of hematopoietic stem cells and mesenchymal stem cells (MSC). Clinical studies shows that MSC can be used in regenerative medicine hereby treatment of cardiac diseases. The aim of this project is to establish a protocol for isolation of MSCs from frozen UCB. This study demonstrated that it’s possible to expand MSC.

A high-throughput colony formation assay for profiling novel compounds and RNAi reagents using the Acumen® eX3
Andrew Goulter and Jason Mundin

Cell colony formation assays measure a cell's ability to grow unattached to a surface and have applications in a range of areas including hematopoietic stem cell research, cell transformation studies and the prediction of responses of tumors to chemotherapeutic agents. The results of this study demonstrated that Acumen eX3 can be used as a high-throughput platform for investigation of effects of test compounds and RNAi reagents on cell colony formation.

Gene Expression Profiling of Archived FFPE Samples
Silvia Rüberg, Sabine Classen, Jana Ciomperlik, Dirk Dietrich, Ines Dischinger, Alena Böttcher, Sabrina Schmitz and Bernhard Gerstmayer

According to the BBMRI (Biobanking and Biomolecular Resources Research Infrastructure) about 8,000,000 formalin-fixed paraffin-embedded (FFPE) samples derived from a multitude of different diseases have been collected in medical centers and biobanks all over Europe during the last decades.

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Showing Results 21 - 30 of 79
Scientific News
Promising Stem Cell Therapy
Animal model of breast-to-brain cancer spread allows testing of therapeutic-cell approach.
A New Lease on Life for Prostate Tissue
Rutgers Cancer Institute of New Jersey research results in development of organoid prostate models
Drugs that Activate Brain Stem Cells May Reverse Multiple Sclerosis
NIH-funded study identifies over-the-counter compounds that may replace damaged cells.
New Transitional Stem Cells Discovered
New stem cells are easier to manipulate, could help future research on reproductive problems.
A New Tool for Understanding ALS: Patients’ Brain Cells
Researchers create a free public library of versatile stem cells from ALS patients.
Limber Lungs: One Type of Airway Cell Can Regenerate Another Lung Cell Type
Findings from animal study have implications for disorders such as chronic obstructive pulmonary disease.
Amniotic Stem Cells Demonstrate Healing Potential
Rice University, Texas Children’s Hospital study proves cells promote vasculature in hydrogel therapy.
Characterizing the Micro-Mechanical Properties of Soft Materials, Tissues and Cells
Ernst Breel of Optics11 discusses the current problems with characterizing the mechanical properties of soft materials, focusing on how the company's Piuma Nanoindenter can assist researchers.
Novel Mechanism Controlling Lung Cancer Stem Cell Growth
Moffitt Cancer Center researchers have discovered a novel mechanism that plays an important role in the maintenance of lung cancer stem cells.
The Switch That Might Tame The Most Aggressive Of Breast Cancers
Garvan researchers have found that so-called ‘triple-negative breast cancers’ are two distinct diseases that likely originate from different cell types. They have also found a gene that drives the aggressive disease, and hope to find a way to ‘switch it off’.
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