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Gene Expression Profiling of Archived FFPE Samples
Silvia Rüberg, Sabine Classen, Jana Ciomperlik, Dirk Dietrich, Ines Dischinger, Alena Böttcher, Sabrina Schmitz and Bernhard Gerstmayer

According to the BBMRI (Biobanking and Biomolecular Resources Research Infrastructure) about 8,000,000 formalin-fixed paraffin-embedded (FFPE) samples derived from a multitude of different diseases have been collected in medical centers and biobanks all over Europe during the last decades.

New Culture Medium Creates Immune Tolerance Between Two Allogeneic Tissue Cells
Victor Alexander, PhD.; Anthony Passerini, PhD; Emir Hodzic, PhD.

We discovered that in vitro high dose of Progesterone (P) together with FGFa, EGF, VEGF, LPS had dual effect on Liver tissue cells: increased multiplication of Hepatocytes and suppressed all non-parenchymal (NP) liver tissue cells and created immune tolerance between two allogeneic mice Liver tissue

Role of PPARd in satellite cells and muscle differentiation
Alison Angione and Shihuan Kuang

Investigating the result of PPARd mutation in the myogenic progenitor cells

High content imaging based mapping of stem cell phenotypes
Er Liu, Sebastian Vega, Anthony Kulesa, Jared Bushman, Hak-Joon Sung, Mattew Becker, Joachim Kohn, and Prabhas Moghe

Stem cells possess the ability of self-renewal and multi-lineage differentiation. However, conventional biological assays usually take weeks to months to identify the stem cell fates. Here we present a high content imaging based profiling platform that enables identifying stem cell phenotypes at much early time points. This platform could help monitor and even predict the long term fate of stem cells.

Hepatitis B virus (HBV) and Human immunodeficiency virus (HIV) antibodies detected by peptide microarrays
ahmed Abd El Wahed1, Ulrike Beutling2, Ronald Frank2, Gerhard Hunsmann1, and Hans-Joachim Fritz3

HBV and HIVenv chips with overlapping oligopeptides encompassing the full amino acid sequences of HBV and HIV polypeptides were produced. In addition, a chip displaying a library of random 4608 different 15-mers peptides (4608-RPL) was prepared. Both chips were used for analyzing monoclonal antibodies and sera from HIV- and HBV-infected individuals. 4608-RPL could be used for identifying target sequences of antibodies without prior knowledge of the corresponding immunizing antigen.

SuperNatural: A Database of Available Natural Compounds
Melanie Füllbeck, Mathias Dunkel and Robert Preissner

The majority of marketed drugs are natural compounds or derivatives thereof. The compounds availability is often unclear. Therefore we have compiled a database of ~50,000 natural compounds. Starting point for in silico screenings are about 2,500 well-known, classified natural compounds or imported molecules. Possible medical applications can be detected and about three million conformers computed to account for the flexibility during usage of the 3D-superposition algorithm.

Nucleic Acid Reagents and Experimental Results in the NCBI Probe Database
Svetlana Iazvovskaia, Ilene Karsch Mizrachi, Kirill Rotmistrovsky, and Savani Tatake

Five years ago, the NCBI Probe database (ProbeDB) was established to provide a centralized archive of molecular probes used in biomedical applications. Currently ProbeDB contains around 10 million probes of 65 types including gene silencing agents, in situ hybridization probes, and probes for variation analysis and genome mapping. Presently, ProbeDB is the largest and most extensive database of this type available in public domain.

Regulatory requirements in the development of advanced therapy products (cell-, gene therapy, tissue engineering products)
Marianne Groeneveld

Regulatory authorities are active to ensure a good regulatory environment for somatic cell therapy-, gene therapy- and tissue engineering products (in the EU known as ‘Advanced Therapy Medicinal Products’). Both the EMEA and the FDA have, amongst others, issued guidelines to address the specific aspects of these products

EFFICIENT LARGE SCALE EXPANSION OF HUMAN EMBRYONIC STEM CELLS: COMPARISON OF 4 DIFFERENT SOLUTIONS FOR SINGLE CELL PASSAGING

Human embryonic stem cells (hESC) are promising for tissue engineering (TE) purposes due to their unique properties. However, the current standard mechanical passaging technique is time consuming and labour intensive, thus limiting the rate of hESC expansion and possible TE experiments.

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Showing Results 21 - 30 of 71
Scientific News
‘Stem Cell’ Test Could Identify Most Aggressive Breast Cancers
Testing breast cancer cells for how closely they resemble stem cells could identify women with the most aggressive disease, a new study suggests.
A Gene For Brain Size - Only Found In Humans
Following the traces of evolution: Max Planck Researchers find a key to the reproduction of brain stem cells.
3D-Printed Guides Can Help Restore Function In Damaged Nerves
Scientists at the University of Sheffield have succeeded in using a 3D printed guide to help nerves damaged in traumatic incidents repair themselves.
Neurons Controlling Appetite Made from Skin Cells
Cells provide individualized model for studying obesity and testing treatments.
Safer Methods for Stem Cell Culturing
New study from TSRI shows that certain stem cell culture methods are associated with increased DNA mutations.
World’s First Cancer Stem Cell Model From iPS Cells
Professor Masaharu Seno at the Department of Biotechnology of Okayama University, reports that cancer stem cells produced from mouse iPS cells are actually necessary for the maintenance of the cancer cells themselves.
Stem Cells from Wisdom Teeth Can Be Transformed into Corneal Cells
The cells could potentially be used to treat corneal scarring.
3-D Engineered Bone Marrow Makes Functioning Platelets
Scalable model supports patient-specific treatments, advanced study of blood disorders.
Growing Functioning Brain Tissue In 3D
RIKEN researchers have induced human embryonic stem cells to self-organize into a 3D cerebellum like structure.
Novel Organoid Matrix Enables Long-Term Culture of Human Hepatocytes
Organoid growth matrix to enable long-term culture of genome-stable bipotent stem cells from adult human liver.
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