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Nucleic Acid Reagents and Experimental Results in the NCBI Probe Database
Svetlana Iazvovskaia, Ilene Karsch Mizrachi, Kirill Rotmistrovsky, and Savani Tatake

Five years ago, the NCBI Probe database (ProbeDB) was established to provide a centralized archive of molecular probes used in biomedical applications. Currently ProbeDB contains around 10 million probes of 65 types including gene silencing agents, in situ hybridization probes, and probes for variation analysis and genome mapping. Presently, ProbeDB is the largest and most extensive database of this type available in public domain.

Regulatory requirements in the development of advanced therapy products (cell-, gene therapy, tissue engineering products)
Marianne Groeneveld

Regulatory authorities are active to ensure a good regulatory environment for somatic cell therapy-, gene therapy- and tissue engineering products (in the EU known as ‘Advanced Therapy Medicinal Products’). Both the EMEA and the FDA have, amongst others, issued guidelines to address the specific aspects of these products

EFFICIENT LARGE SCALE EXPANSION OF HUMAN EMBRYONIC STEM CELLS: COMPARISON OF 4 DIFFERENT SOLUTIONS FOR SINGLE CELL PASSAGING

Human embryonic stem cells (hESC) are promising for tissue engineering (TE) purposes due to their unique properties. However, the current standard mechanical passaging technique is time consuming and labour intensive, thus limiting the rate of hESC expansion and possible TE experiments.

Immunomodulatory Properties of Adipose-Derived Stem Cells Cultured in Autologous Serum
Eivazova G, Berzins U, Zvaigzne A, Skrastina D, Bogdanova A, Kozlovska T, Lejnieks A

This study investigated whether adipose-derived stem cells (ADSC), cultivated in the medium containing autologous serum, maintain immunomodulatory properties of MSC. Obtained cells were CD73, CD90 and CD105 positive but CD34 and CD45 negative. ADSC underwent in vitro differentiation into adipocytes, osteoblasts and chondroblasts. Immunomodulatory properties of ADSC were investigated by blast transformation reaction and significant suppression of T lymphocyte proliferation was induced in a dose d

Development of cell culture system on the basis of adult stem cells for modeling of HBV infection
Zajakina A, Berzins U, Bogdanova A, Kozlovska T

Efforts to elucidate the mechanisms by which HBV infects hepatocytes and causes liver damage have been hampered by the strict virus host range and a lack of suitable cell culture and animal models of HBV infection/replication. The present study has established and elucidated a novel HBV infection/replication in vitro model system based on adipose-derived stem cells differentiated into human hepatocyte-like cell line.

FOXP3 Gene Expression in Multiple Sclerosis patients before and after Mesenchymal Stem Cell therapy
Maryam Mohajeri, Mandana Mohyeddin Bonab, Behrooz Nikbin, Ali Farazmand

Multiple Sclerosis (MS) is a chronic inflammatory demyelinating disorder of the CNS. No successful treatment for MS, but one therapeutic strategy in research is the use of bone marrow-derived mesenchymal stem cells (MSC). We studied a group of MS patients who underwented MSCs, assayed for expression of a transcription factor, FOXP3, as a specific marker of MS amelioration in peripheral blood. qRT-PCR on PBMCs showed higher FoxP3 levels.

Three-Dimensional Cell Culture Within A Biomimetic Hydrogel Based On Chitosan And Hyaluronan

A growing demand for study of cells within biologically realistic, in vitro, microenvironments requires a paradigm shift in cell culture technology: from cell culture on 2-D, rigid substrata to cell growth within a 3-D space, defined by malleable materials composed of biologically “smart” compounds.1, 2 Therapeutic issues addressed by tissue engineering and regenerative medicine require similar materials, customized for human application in a wide variety of treatment circumstances: ranging

Characterization of enamel and dentin protein expression in bioengineered human tooth tissues
Sheila Soroushian1, Monica Duailibi2, Silvio Duailibi2, Weibo Zhang1, Robert Litterman1, Ivy Pruit1, Pamela C. Yelick1

Here we have used previously described methods to bioengineer teeth from human dental stem cells (hDSC). We we were able to demonstrate that dissociated hDSC-seeded polymer constructs can form bioengineered teeth that are morphologically similar to natural human teeth at both gross and microscopic levels. We anticipate that further modifications of this approach will eventually result in reliable methods to bioengineer replacement teeth in humans.

The advancement of feeder-independent culture systems for undifferentiated human pluripotent stem cells towards greater regulatory compliance

Defined and feeder-independent cell culture systems provide a platform for greater reproducibility and standardization in human pluripotent stem cell (hPSC) research, mTeSR 1 has become the most widely cited feeder independent system for the culture of undifferentiated human ES and iPSC.

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Showing Results 41 - 50 of 85
Scientific News
AACR 2016: Cancer Immunotherapy and Beyond
At this year's meeting there was a palpable buzz around subjects ranging from microbiomics to the tumor microenvironment and cancer vaccines, big data to in vitro and in vivo modeling and drug delivery (to name just a few).
How Skeletal Stem Cells Form The Blueprint Of The Face
USC researchers discover that two types of molecular signals work to control where and when stem cells turn into facial cartilage.
Turning Skin Cells into Heart, Brain Cells
In a major breakthrough, scientists at the Gladstone Institutes transformed skin cells into heart cells and brain cells using a combination of chemicals.
Stem Cells Know How to Unwind
Research led by the Babraham Institute with collaborators in the UK, Canada and Japan has revealed a new understanding of how an open genome structure supports the long-term and unrestricted developmental potential in embryonic stem cells.
Growing Stem Cells More Safely
Nurturing stem cells atop a bed of mouse cells works well, but is a non-starter for transplants to patients – Brown University scientists are developing a synthetic bed instead.
Cell Transplant Treats Parkinson’s in Mice
A University of Wisconsin—Madison neuroscientist has inserted a genetic switch into nerve cells so a patient can alter their activity by taking designer drugs that would not affect any other cell.
Skin Cells Turned into Heart Cells and Brain Cells Using Drugs
In a scientific first, Gladstone researchers have used chemical drugs to convert skin cells into heart cells and brain cells, without adding any external genes.
Shape Of Tumor May Affect Whether Cells Can Metastasize
Illinois researchers found that the shape of a tumor may play a role in how cancer cells become primed to spread.
‘Mini-Brains’ to Study Zika
Novel tool expected to speed research on brain and drug development.
Cytokine Triggers Immune Response at Expense of Blood Renewal
Research highlights promise of Anti-IL-1 drugs to treat chronic inflammatory disease.
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