|CyTRAK™ Probes: Novel Nuclear and Cytoplasm Discriminators Compatible with GFP-Based HCS and HTS Assays|
RJ Errington, LH Patterson, R Edward and PJ Smith
Image-based high-content screening assays, demand solutions for image segmentation and cellular compartment encoding to track critical events – for example those presented by GFPreporters within cell cycle tracking and GPCR translocation assays. We have designed nuclear and cytoplasm discriminator CyTRAK™ probes - spectrally compatible with all variants of GFPreporters offering new solutions in cytometry.
|A novel Hydrogel Mountant - CyGEL™ - Enables Temporospatial HCS Imaging Assays of Live non-Adherent Cells|
Edward R, Errington RJ, Patterson LH, Ogrodzinski S and Smith PJ
New technologies are needed to deal with live cell-based HCS assays on non-adherent cells or to exploit suspension cells in fluidic systems. Here we describe CyGEL™ - a thermo-reversible hydrogelbased 4-D immobilization technology for live cell location without imposing an anchoring to a substrate.
|DNA Methylation Analysis – Reliable Cell Characterization in Regenerative Medicine|
Uli Hoffmueller, Stephen Rapko, Udo Baron, Georg Wieczorek, Alexander Hellwag, Cornelia Krüger, Stefan Kärst, Leslie Wolfe and Sven Olek
We demonstrate that DNA methylation patterns can serve as characteristic markers to distinguish different cell types. We have identified panels of methylation markers that are specific to mesenchymal stem cells or various differentiated cell types in the mesenchymal lineage. This method of cell type identification has a number of advantages over conventional markers in that it is robust, is both qualitative and quantitative.
|An Emerging Revolution in Automated 3D Cell Culture|
Cal Trepagnier, Stacey Szymanski, Kevin Huff, Alision Rush, Richard Peltier, Berta strulovici, Rebecca Lobo and Robin Felder
Market pressure exists for new paradigms to meet the demands for more rapid and reproducible cell production to keep up with advances in cell-based assays used in drug discovery and development. Novel automation platforms enable high productivity on three dimensional surfaces.
|The Effect of Microwell size and Inoculation Density on the Automated Processing of Stem Cells|
Rosario Scott, Farlan S Veraitch, Spyridon Gerontas, Gary J Lye and Chris Mason
The work presented in this poster covers the investigation of key parameters for the expansion of stem cells in an automated micro-well format. The effect of inoculation seeding density (ICD), well size, passage number and feeding strategies on the proliferation of human mesenchymal stromal cells (hMSC) was investigated. In parallel the effect of well size and seeding density on the proliferation of mouse embryonic stem (mES) cells was also examined.
|Identification of Chemokines and Cytokines Involved in the Migration of MSC to Bone Marrow as Well as to Damaged Tissues|
Tondreau T, Meuleman N, Massy M, Delforge A, Bron D and Lagneaux L.
Recent in vivo studies have demonstrated that transplanted MSC in mice were able to migrate into various tissues including lung, brain, bone marrow, heart and many others. Nevertheless, mechanisms responsible for the MSC migration remain unclear.
|DNA Microarrays for Microbiological Diagnosis in Stem Cell Cultures|
F. Cobo, A. Nieto, P. Catalina, JL. Cortés, A. Barroso, C. Cabrera, R. Montes, A. Barnie and A. Concha
In stem cell cultures there is the possibility of infectious disease transmission to the recipients. Any microbial contamination of the donor`s biological products or introduced during the manufacturing process can potentially present a serious hazard to the recipients. The majority of potential contaminants are mycoplasma and other bacterias, yeasts and fungi. Moreover, viral and prion contamination of cell cultures and “feeder” cells is also a common risk.
|In vitro Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Towards the Osteoblast Lineage. A Model to Study the Effects of Cancer Cells on Bone Formation|
Hichame Id Boufker, Fabrice Journé, Laurence Lagneaux, Tatiana Tondreau and Jean-Jacques Body
This poster describes an in vitro model of DAG-induced osteogenic differentiation of bone marrow mesenchymal stem using various osteoblastic markers. Human MSC should constitute a useful in vitro tool to the effects of osteotropic cancer cells on osteoblast differentiation and to better understand the inhibition of normal formation during the process of tumor-induced osteolysis.
|Microbiological Control in a Stem Cell Bank: Standardisation|
F. Cobo, A. Nieto, R.M. Montes, C. Cadrera, A. Barnie, J.L.Cortes, P. Catalina, L. Gutierrez, R. Lopez and A. Concha
The transplant of cells of human origin is a sector of medicine which shows a high increase and which entails great opportunities for treatment of certain diseases. Stem cell banks should assure the quality, the traceability and the safety of cultures in order to avoid transmission of disease.
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