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Tuesday, September 30, 2014
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CyTRAK™ Probes: Novel Nuclear and Cytoplasm Discriminators Compatible with GFP-Based HCS and HTS Assays
RJ Errington, LH Patterson, R Edward and PJ Smith

Image-based high-content screening assays, demand solutions for image segmentation and cellular compartment encoding to track critical events – for example those presented by GFPreporters within cell cycle tracking and GPCR translocation assays. We have designed nuclear and cytoplasm discriminator CyTRAK™ probes - spectrally compatible with all variants of GFPreporters offering new solutions in cytometry.

A novel Hydrogel Mountant - CyGEL™ - Enables Temporospatial HCS Imaging Assays of Live non-Adherent Cells
Edward R, Errington RJ, Patterson LH, Ogrodzinski S and Smith PJ

New technologies are needed to deal with live cell-based HCS assays on non-adherent cells or to exploit suspension cells in fluidic systems. Here we describe CyGEL™ - a thermo-reversible hydrogelbased 4-D immobilization technology for live cell location without imposing an anchoring to a substrate.

DNA Methylation Analysis – Reliable Cell Characterization in Regenerative Medicine
Uli Hoffmueller, Stephen Rapko, Udo Baron, Georg Wieczorek, Alexander Hellwag, Cornelia Krüger, Stefan Kärst, Leslie Wolfe and Sven Olek

We demonstrate that DNA methylation patterns can serve as characteristic markers to distinguish different cell types. We have identified panels of methylation markers that are specific to mesenchymal stem cells or various differentiated cell types in the mesenchymal lineage. This method of cell type identification has a number of advantages over conventional markers in that it is robust, is both qualitative and quantitative.

An Emerging Revolution in Automated 3D Cell Culture
Cal Trepagnier, Stacey Szymanski, Kevin Huff, Alision Rush, Richard Peltier, Berta strulovici, Rebecca Lobo and Robin Felder

Market pressure exists for new paradigms to meet the demands for more rapid and reproducible cell production to keep up with advances in cell-based assays used in drug discovery and development. Novel automation platforms enable high productivity on three dimensional surfaces.

The Effect of Microwell size and Inoculation Density on the Automated Processing of Stem Cells
Rosario Scott, Farlan S Veraitch, Spyridon Gerontas, Gary J Lye and Chris Mason

The work presented in this poster covers the investigation of key parameters for the expansion of stem cells in an automated micro-well format. The effect of inoculation seeding density (ICD), well size, passage number and feeding strategies on the proliferation of human mesenchymal stromal cells (hMSC) was investigated. In parallel the effect of well size and seeding density on the proliferation of mouse embryonic stem (mES) cells was also examined.

Identification of Chemokines and Cytokines Involved in the Migration of MSC to Bone Marrow as Well as to Damaged Tissues
Tondreau T, Meuleman N, Massy M, Delforge A, Bron D and Lagneaux L.

Recent in vivo studies have demonstrated that transplanted MSC in mice were able to migrate into various tissues including lung, brain, bone marrow, heart and many others. Nevertheless, mechanisms responsible for the MSC migration remain unclear.

DNA Microarrays for Microbiological Diagnosis in Stem Cell Cultures
F. Cobo, A. Nieto, P. Catalina, JL. Cortés, A. Barroso, C. Cabrera, R. Montes, A. Barnie and A. Concha

In stem cell cultures there is the possibility of infectious disease transmission to the recipients. Any microbial contamination of the donor`s biological products or introduced during the manufacturing process can potentially present a serious hazard to the recipients. The majority of potential contaminants are mycoplasma and other bacterias, yeasts and fungi. Moreover, viral and prion contamination of cell cultures and “feeder” cells is also a common risk.

In vitro Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Towards the Osteoblast Lineage. A Model to Study the Effects of Cancer Cells on Bone Formation
Hichame Id Boufker, Fabrice Journé, Laurence Lagneaux, Tatiana Tondreau and Jean-Jacques Body

This poster describes an in vitro model of DAG-induced osteogenic differentiation of bone marrow mesenchymal stem using various osteoblastic markers. Human MSC should constitute a useful in vitro tool to the effects of osteotropic cancer cells on osteoblast differentiation and to better understand the inhibition of normal formation during the process of tumor-induced osteolysis.

Microbiological Control in a Stem Cell Bank: Standardisation
F. Cobo, A. Nieto, R.M. Montes, C. Cadrera, A. Barnie, J.L.Cortes, P. Catalina, L. Gutierrez, R. Lopez and A. Concha

The transplant of cells of human origin is a sector of medicine which shows a high increase and which entails great opportunities for treatment of certain diseases. Stem cell banks should assure the quality, the traceability and the safety of cultures in order to avoid transmission of disease.

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Showing Results 61 - 70 of 70
Scientific News
How Common Mutation in Asians Affects Heart Health
Researchers studied heart muscle cells derived from pluripotent stem cells to find out why a genetic mutation common in East Asians leads to an increased risk of heart disease.
How Gene Expression Affects Facial Expressions
Researchers from USC have demonstrated how a mutation in TBX1 causes facial deformities associated with DiGeorge Syndrome.
Stem Cells Use “First Aid Kits” to Repair Damage
Neural stem cells - master cells that can develop into any type of nerve cell - are able to generate mini “first aid kits” and transfer them to immune cells, according to a study published.
Reliable and Highly Efficient Method for Making Stem Cells
New finding could accelerate research to regenerate damaged tissue.
A New Molecule Allows for an Increase in Stem Cell Transplants
A clinical study using UM171 and a new type of bioreactor developed for stem culture will be initiated in December 2014.
Novel Drug Targeting Leukemia Cells Enters Clinical Trial
Phase 1 human clinical trial to assess the safety and efficacy of a new monoclonal antibody for CLL patients.
What Lies Behind the Death of Stem Cells
Researchers have identified key processes that control stem cell survival, providing insights that could improve their use in medicine.
Scientists Discover Neurochemical Imbalance in Schizophrenia
Researchers discovers that neurons from schizophrenia patients secrete higher amounts of three neurotransmitters.
Building Replacement Kidneys in the Lab
Milestone reached in work to build replacement kidneys in the lab.
Diabetes Researchers Find Faster Way to Create Insulin-producing Cells
A new protocol can turn stem cells into reliable, insulin-producing cells in about six weeks.
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