Corporate Banner
Satellite Banner
Stem Cells, Cellular Therapy & Biobanking
>
Scientific Community
 
Become a Member | Sign in
Home>Products>This Product
  Products


Human Nanog Differentiation Reporter (pGreenZeo, pre-packaged)

Product Description
Accurately monitor the pluripotent state Mammalian development requires the specification of over 200 unique cell types from a single totipotent cell. Embryonic stem (ES) cells are derived from the inner cell mass of the developing blastocyst and can be propagated in culture in an undifferentiated state while maintaining the capacity to generate any cell type in the body. The recent derivation of human ES cells provides a unique opportunity to study early development and an understanding of the transcriptional regulatory circuitry that is responsible for pluripotency and self-renewal in human ES cells is fundamental to understanding human development and realizing the therapeutic potential of these cells. Three transcription factors are known to be critical in the maintenance of ESC pluripotency: Oct4, Nanog, and Sox2. Oct4 (Pou5f1) has a highly conserved role in maintaining pluripotent cell populations (Nichols et al., 1998; Morrison and Brickman, 2006), and its expression level dictates ESC fate (Niwa et al., 2000). SOX2 forms a complex with OCT4 and is necessary to cooperatively activate target genes in ESCs (Yuan et al., 1995; Ambrosetti et al., 1997). These factors comprise one essential circuit regulating ESC pluripotency in which OCT4 regulates Sox2, and additionally, the OCT4-SOX2 complex activates Oct4 expression (Okumura-Nakanishi et al., 2005). The transcription factors OCT4, SOX2, and NANOG have essential roles in early development and are required for the propagation of undifferentiated embryonic stem (ES) cells in culture. SBI has built lentivector-based transciptional Response Reporters that are responsive to Oct4 or Sox2 activity as response elements or Promoter Reporters for either Human or Mouse Oct4 and Nanog into the pGreenZeo, pRedZeo and pRedTK systems. Choice of fluorescent screening marker The lenti-reporter systems employ either a Green Fluorescent Protein (copGFP) or a Red Fluorescent Protein (RFP). Depending upon your screen, either Green or Red markers may be suitable and compatible with other fluorescent protein markers in your experimental scheme. The Response Reporters feature the dual reporting system with both GFP and Luciferase. Choice of antibiotic selection marker The Response Reporters have an optional constitutive EF1-Puro or -Neo selection cassette for establishing cells lines. The Promoter Reporters feature Zeomycin (Zeo) resistance marker confers resistance to the antibiotic Zeocin™. Zeocin™ causes cell death by intercalating into DNA and causes DNA strand breakage. This antibiotic is effective on most aerobic cells and is therefore useful for selection in bacteria, eukaryotic microorganisms, plant and animal cells. Thymidine kinase (TK) is a Herpes simplex virus-derived enzyme that acts on the guanosine nucleotide analog ganciclovir. TK phosphorylates ganciclovir, and TK+ cells produce highly-toxic triphosphates that lead to cell death (negative selection). All Stem Cell Pluipotency Monitors are available as lentivector plasmids or as pre-packaged lentiviral preparations (using the HIV lentiviral backbone and pseudotyped with VSV-G protein)—ready for transduction of target cells. The standard product size is 10 ug of plasmid or 1 x 10^6 IFUs per packaged lentiviral reporter. Positive and negative transduction control plasmids and pre-packaged viral particles are also available.
Product Human Nanog Differentiation Reporter (pGreenZeo, pre-packaged)
Company System Biosciences
Price Request a quote
More Information View company product page
Catalog Number SR10030VA-1
Quantity >2 x 10^6 IFUs
Company Logo

System Biosciences
265 North Whisman Rd. Mountain View, CA 94043, USA

Tel: 1.650-968-2200
Fax: 1.650.968.2277
Email: info@systembio.com



Scientific News
Bile Acid Supports Production of Blood Stem Cells
A research group at Lund University has been able to show that bile acid is transferred from the mother to the foetus via the placenta to enable the foetus to produce blood stem cells.
New Biomarker to Assess Stem Cells Developed
A research team led by scientists from UCL have found a way to assess the viability of 'manufactured' stem cells known as induced pluripotent stem cells (iPSCs). The team's discovery offers a new way to fast-track screening methods used in stem cell research.
Tricked-Out Immune Cells Could Attack Cancer
New cell-engineering technique may lead to precision immunotherapies.
Edited Stem Cells Offer Hope of Precision Therapy for Blindness
Findings raise the possibility of treating blinding eye diseases using a patient's own corrected cells as replacement tissue.
Hacking the Programs of Cancer Stem Cells
All tumor cells are the offspring of a single, aberrant cell, but they are not all alike.
Newfound Strength in Regenerative Medicine
A promising new approach uses direct mechanical stimulation to repair severely damaged skeletal muscles.
Mapping out Cell Conversion
Researchers develop algorithm that takes the field of cell reprogramming forward.
Donor's Genotype Controls the Differentiation of IPS Cells
Pluripotent stem cells derived from different cell types are equally susceptible to reprogramming, indicates a recent study by the University of Helsinki and the National Institute for Health and Welfare, Finland. However, the genotype of the donor strongly influences the differentiation of the stem cell.
Signals That Make Early Stem Cells Identified
Researchers at The Rockefeller University have identified a new mechanism by which cells are instructed during development to become stem cells
Healing Scarred Hearts
Findings suggest stem cells may one day be used to regenerate damaged tissue after heart attack.

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!