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Mitochondria Staining Kit

Product Description
In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.

Features and Benefits

Kit offers:
• A fast, simple, and convenient method for staining and assaying mitochondria intactness
• Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential
• Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation
• The shift in fluorescence is clearly detectable in both green and red channels
• Suitable for adherent cells as well as cells in suspension
• Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells
• The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry
Product Mitochondria Staining Kit
Company Sigma Aldrich
Price Request a quote
More Information View company product page
Catalog Number CS0390
Quantity 1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)
Company Logo

Sigma Aldrich
Sigma-Aldrich 3050 Spruce St. St. Louis, MO 63103 US

Tel: 800-325-3010
Fax: 800-325-5052
Email: cssorders@sial.com



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