In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.
Features and Benefits
Kit offers: • A fast, simple, and convenient method for staining and assaying mitochondria intactness • Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential • Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation • The shift in fluorescence is clearly detectable in both green and red channels • Suitable for adherent cells as well as cells in suspension • Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells • The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry