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Nuclei Isolation Kit: Nuclei PURE Prep

Product Description
For preparation of pure nuclei and fragile nuclei from cell lines and solid tissues.

Biochem/physiol Actions

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. The sucrose concentration that is suitable for a particular cell type is determined empirically by the user. The sucrose concentrate and sucrose cushion buffer give the user flexibility to modify the density of the sucrose cushion as appropriate. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.
Product Nuclei Isolation Kit: Nuclei PURE Prep
Company Sigma Aldrich
Price Request a quote
More Information View company product page
Catalog Number NUC201-1KT
Quantity Sufficient for 15 nuclei preparations (~1-10×10^7 cells or 1g of tissue per preparation)
Company Logo

Sigma Aldrich
Sigma-Aldrich 3050 Spruce St. St. Louis, MO 63103 US

Tel: 800-325-3010
Fax: 800-325-5052
Email: cssorders@sial.com



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