Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Join | Sign in
Home>Videos>This Video
  Videos

Return

Old Structures - New Targets for Antivirulence Salicylidene Acylhydrazide Compounds
Select Biosciences Ltd

Salicylidene acylhydrazide compounds have been demonstrated to perturb the function of the type three secretion of many important human pathogens including Escherichia coli O157 and Yersinia pseudotuberculosis. The actual protein targets of these compounds had, to this date, been unknown. In this work we present the identification and verification of three such protein targets. Each protein was identified using a combination of affinity chromatography and Far-western analyses followed by phenotypic testing of defined bacterial mutants using in vitro and in vivo assays. , , The structure of the target protein with the greatest effect on virulence in Yersinia pseudotuberculosis was solved in 3 forms by X-ray crystallography. In combination with studies of inactive mutants, and conformational changes in the protein caused by redox active state, we present localised regions likely to bind to the drug. Work is under way to solve the structure of protein and compound that will permit refinement of the salicylidene acylhydrazides with the long-term goal of developing clinically-useable drugs.

Request more information
Company product page


Access to this article and other content is for registered users.

Join the Technology Networks Community

  • Access to the latest scientific news, products and research through Technology Networks
  • Upload and share your posters on ePosters
  • View a library of 1,800+ scientific and medical posters
  • A library of 3,000+ scientific videos on LabTube


Sign In



Forgotten your details? Click Here
If you already have an account with Technology Networks, please use your existing login details. If you do not yet have an account please join here.

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Scientific News
Finding Points To A Cause Of Chronic Lung Disease
New research explains how macrophages in the lung sometimes stick around too long, even after clearing a viral infection, leading to long-term lung problems.
A New Wrinkle For Cell Culture
Researchers at Brown University have developed an advanced technique for cell culturing that uses sheets of wrinkled graphene to mimic the complex 3-D environment inside the body.
Twins Experiment Reveals Genetic Link with Mosquito Bites
Findings show that attractiveness to insects is based on differences in body odor.
Detecting Cryptosporidium in China
A recently developed lab-on-a-chip device, which can diagnose the opportunistic parasite in as little as 10 minutes, may help improve treatment in remote, at-risk rural areas.
Quotient Clinical Completes Innovative First-In-Human Program
First-in-human clinical program designed to develop an optimal oral formulation.
Detecting Low-Quality Antimalarial Drugs With A Lab-On-Paper
Lab-on-Paper can detect low-quality anitmalarial drugs without expensive equipment.
A New Lease on Life for Prostate Tissue
Rutgers Cancer Institute of New Jersey research results in development of organoid prostate models
Imaging Immunity
Noninvasive imaging of immune system detects tumors, could monitor therapeutic response.
First Atlas Of Airborne Microbes Across US
Bacteria and fungal species were identified from outdoor dust samples collected from all 50 states.
Measuring Volumes of Key 'Lab on a Chip' Components
NIST found a combination of techniques to effectively measure microfluidic channels, achieving an accuracy of within 5 percent for both a channel's depth and its bottom's width.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters