Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Join | Sign in
Home>Videos>This Video
  Videos

Return

Old Structures - New Targets for Antivirulence Salicylidene Acylhydrazide Compounds
Select Biosciences Ltd

Salicylidene acylhydrazide compounds have been demonstrated to perturb the function of the type three secretion of many important human pathogens including Escherichia coli O157 and Yersinia pseudotuberculosis. The actual protein targets of these compounds had, to this date, been unknown. In this work we present the identification and verification of three such protein targets. Each protein was identified using a combination of affinity chromatography and Far-western analyses followed by phenotypic testing of defined bacterial mutants using in vitro and in vivo assays. , , The structure of the target protein with the greatest effect on virulence in Yersinia pseudotuberculosis was solved in 3 forms by X-ray crystallography. In combination with studies of inactive mutants, and conformational changes in the protein caused by redox active state, we present localised regions likely to bind to the drug. Work is under way to solve the structure of protein and compound that will permit refinement of the salicylidene acylhydrazides with the long-term goal of developing clinically-useable drugs.

Request more information
Company product page


Access to this article and other content is for registered users.

Join the Technology Networks Community

  • Access to the latest scientific news, products and research through Technology Networks
  • Upload and share your posters on ePosters
  • View a library of 1,800+ scientific and medical posters
  • A library of 3,000+ scientific videos on LabTube


Sign In



Forgotten your details? Click Here
If you already have an account with Technology Networks, please use your existing login details. If you do not yet have an account please join here.

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Scientific News
Personalized Screening for Ovarian Cancer
With 60% of women diagnosed with ovarian cancer dying within five years of diagnosis there has been considerable efforts to try to detect the disease at an earlier stage.
Gene Therapy for Cystic Fibrosis Shows Encouraging Trial Results
A therapy that replaces the faulty gene responsible for cystic fibrosis in patients' lungs has produced encouraging results in a major UK trial.
In Blinding Eye Disease, Trash-Collecting Cells Go Awry, Accelerate Damage
NIH research points to microglia as potential therapeutic target in retinitis pigmentosa.
Artificial Pancreas Controls Diabetes
Scientists are reporting the development of an implantable “artificial pancreas” that continuously measures a person’s blood sugar, or glucose, level and can automatically release insulin as needed.
Growing Spinal Disc Tissue
Scientists develop new method for growing spinal disc tissue in the lab for combating chronic back pain.
Imaging Individual Molecules
JILA researchers have designed a microscope instrument so stable that it can accurately measure the 3D movement of individual molecules over many hours-hundreds of times longer than the current limit measured in seconds.
Long-term Memories Are Maintained by Prion-like Proteins
Research from Eric Kandel’s lab at Columbia University Medical Center has uncovered evidence of a system in the brain that persistently maintains memories for long periods of time.
How the Mammoth Got its Wool
Evolutionary change in a gene reconstructed in the lab from the woolly mammoth was part of a suite of adaptations that allowed the mammoth to survive in harsh arctic environments, according to new research.
Chemists Design a Quantum-Dot Spectrometer
New instrument is small enough to function within a smartphone, enabling portable light analysis.
Elastic Gel to Heal Wounds
A team of bioengineers at Brigham and Women’s Hospital has developed a new protein-based gel that, when exposed to light, mimics many of the properties of elastic tissue, such as skin and blood vessels.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters