Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Videos>This Video
  Videos

Return

The Epigenetic Regulation of microRNA Genes and Their Role in Cell Invasion and Motility in Human Melanomas
Sandford Burnham Medical Research Institute

MicroRNAs have joined the ranks of important regulatory molecules known to control human cell fate in normal and diseased states. This is also true of cancer cells, including melanoma cells, which are known to undergo progressive epigenetic changes during disease manifestation.   We have identified a group of epigenetically regulated miRNA genes in melanoma cells, and employing direct DNA bisulphite and immunoprecipitated methylated DNA (methyl-DIP) deep-sequencing we have confirmed that the upstream CpG island sequences of several such miRNA genes are hypermethylated in cell lines derived from different stages of melanoma but not in melanocytes and keratinocytes.  Abnormalities in CpG island methylation were also seen in distinct melanoma patient samples classified as primary in situ, regional metastatic, distant metastatic, and nodal metastatic.  The cell line WM1552C derived from a stage 3 melanoma was engineered to artificially express one such epigenetically modified miRNA, miR-34b. This caused a reduction in cell invasion and proliferation rates, suggesting that reduced expression of miR-34b is related to invasiveness and high cell division rate of WM1552C.  RNA samples isolated from WM1552C with or without a miR-34b expression construct were subjected to deep sequencing to identify gene networks around miR-34b. Network modules potentially regulated by miR-34b are related to cytoskeletal remodeling, cell invasion and cell mortality functions, suggesting a mechanism for the role of miR-34b in regulating normal cell motility and cytokinesis.

Request more information
Company product page


Scientific News
Criminal Justice Alcohol Program Linked to Decreased Mortality
Institute has announced that in the criminal justice alcohol program deaths dropped by 4.2 percent over six years.
Charting Kidney Cancer Metabolism
Changes in cell metabolism are increasingly recognized as an important way tumors develop and progress, yet these changes are hard to measure and interpret. A new tool designed by MSK scientists allows users to identify metabolic changes in kidney cancer tumors that may one day be targets for therapy.
Improving Regenerative Medicine
Lab-created stem cells may lack key characteristics, UCLA research finds.
Tick Genome Reveals Secrets of a Successful Bloodsucker
NIH has announced that decipher the genome of the blacklegged tick which could lead to new tick control methods.
"Dark Side" of the Transcriptome
New approach to quantifying gene "read-outs" reveals important variations in protein synthesis and has implications for understanding neurodegenerative diseases.
Individuals' Medical Histories Predicted by their Noncoding Genomes
Researchers have found that analyzing mutations in regions of the genome that control genes can predict medical conditions such as hypertension, narcolepsy and heart problems.
'Molecular Movie' Opens Door to New Cancer Treatments
An international team of scientists led by the University of Liverpool has produced a 'structural movie' revealing the step-by-step creation of an important naturally occurring chemical in the body that plays a role in some cancers.
New Source of Mutations in Cancer
Recently, a new mutation signature found in cancer cells was suspected to have been created by a family of enzymes found in human cells called the APOBEC3 family.
Advancing Synthetic Biology
Living systems rely on a dizzying variety of chemical reactions essential to development and survival. Most of these involve a specialized class of protein molecules — the enzymes.
Madison Researchers Begin Work on Zika Virus
Work will start with basic questions about Zika virus infection.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!