Corporate Banner
Satellite Banner
Technology Networks Header
Monday, November 24, 2014
 
Register | Sign in
Home Page > Videos > Challenges of Analyzing Single Cell Gene Expression Data
  Videos

Return

Challenges of Analyzing Single Cell Gene Expression Data
Fluidigm Corporation

Kenneth Livak, Senior Scientific Fellow, Fluidigm

Abstract
Single cell researchers are currently using the Fluidigm BioMark System to measure gene expression levels for 10s to 100s of genes in 100s to 1000s of samples.  Data from single eukaryotic cells show cell-to-cell variation in mRNA amounts that ranges from 10- to 1000-fold depending on the gene and type of cell.  These results fit the stochastic model that eukaryotic transcripts are produced in short but intense bursts interspersed with intervals of inactivity.  This noise inherent in single cell gene expression challenges conventional methods for obtaining and analyzing qPCR data.  Factors such as replicates, limit of detection, normalization, data display, and univariate versus multivariate analysis need to be re-evaluated.  Another important consideration is that data needs to be collected from a statistically significant number of single cells and for at least tens to hundreds of genes.  By acknowledging and addressing the intrinsic noise, single-cell gene expression profiling can be used to gain biological insights that are just not possible when measuring average expression levels from hundreds or thousands of cells.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Brain-dwelling Worm in UK Man's Head Sequenced
Tapeworm removed from UK resident's brain reveals genetic secrets of an elusive Far East parasite.
Researchers Tease Out Glitches in Immune System's Self-Recognition
A new study revises understanding of how the process works and sheds light on autoimmune disease.
Crowdsourcing Science To Beat Psa
Scientists are hoping that crowdsourcing knowledge from the international science community will help them find the answer to a lethal kiwifruit disease.
Breakthrough in Managing Yellow Fever Disease
UC Riverside-led research could lead to antiviral therapeutics and better diagnostics to prevent spread of potentially fatal viral infection.
Themis Bioscience’s Vaccine Against Chikungunya Successful in Phase 1
Results presented at international conferences in Philadelphia and New Orleans.
Similarities and Striking Differences Found Between Mouse and Human Genome
Comparisons of both genomes’ inner workings may lead to a better use of mouse models.
New Strategy To Improve Stem Cell Transplantation
Research addresses hurdles to future transplant-based, gene-editing therapies that could help people with genetic blood diseases.
Molecular Event Mapping Opens Door to more in silico Tests
It is hoped that this new approach to mapping and predicting the impact of chemical compounds in the body could reduce the need for toxicity tests in animals.
Big Data Helps Pinpoint Possible New Stent Drug
Replacing the current drug used to coat artery-opening stents with a drug more targeted to the actual cause of stent disease could reduce blood clots and heart attacks.
Anti-Leukemia Drug May Also Work Against Ovarian Cancer
An antibody therapy already in clinical trials to treat chronic lymphocytic leukemia (CLL) may also prove effective against ovarian cancer – and likely other cancers as well.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv