Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>Videos>This Video


Challenges of Analyzing Single Cell Gene Expression Data
Fluidigm Corporation

Kenneth Livak, Senior Scientific Fellow, Fluidigm

Single cell researchers are currently using the Fluidigm BioMark System to measure gene expression levels for 10s to 100s of genes in 100s to 1000s of samples.  Data from single eukaryotic cells show cell-to-cell variation in mRNA amounts that ranges from 10- to 1000-fold depending on the gene and type of cell.  These results fit the stochastic model that eukaryotic transcripts are produced in short but intense bursts interspersed with intervals of inactivity.  This noise inherent in single cell gene expression challenges conventional methods for obtaining and analyzing qPCR data.  Factors such as replicates, limit of detection, normalization, data display, and univariate versus multivariate analysis need to be re-evaluated.  Another important consideration is that data needs to be collected from a statistically significant number of single cells and for at least tens to hundreds of genes.  By acknowledging and addressing the intrinsic noise, single-cell gene expression profiling can be used to gain biological insights that are just not possible when measuring average expression levels from hundreds or thousands of cells.

Request more information
Company product page

Scientific News
Fixing Holes in the Heart Without Invasive Surgery
UV-light enabled catheter is a medical device which represents a major shift in how cardiac defects are repaired.
Chromosomal Chaos
Penn study forms basis for future precision medicine approaches for Sezary syndrome
Enzyme Malfunction May be Why Binge Drinking Can Lead to Alcoholism
A new study in mice shows that restoring the synthesis of a key brain chemical tied to inhibiting addictive behavior may help prevent alcohol cravings following binge drinking.
Key to Natural Detoxifier’s Reactivity Discovered
Results have implications for health, drug design and chemical synthesis.
New Treatment for Obesity Developed
Researchers at the University of Liverpool, working with a global healthcare company, have helped develop a new treatment for obesity.
New Protein Found in Immune Cells
Immunobiologists from the University of Freiburg discover Kidins220/ARMS in B cells and demonstrate its functions.
Will Brain Palpation Soon Be Possible?
Researchers have developed non-invasive brain imaging technique which provides the same information as physical palpation.
Shaking Up the Foundations of Epigenetics
Researchers at the Centre for Genomic Regulation (CRG) and the University of Barcelona (UB) published a study that challenges some of the current beliefs about epigenetics.
Groundbreaking Computer Program Diagnoses Cancer in Two Days
Researchers have combined genetics with computer science and created a new diagnostic technology can with 85 per cent certainty identify the source of the disease and thus target treatment and, ultimately, improve the prognosis for the patient.
Michigan Researchers Use Raman Spectroscopy
inVia confocal Raman microscope used in the study of various childhood diseases.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos