Corporate Banner
Satellite Banner
Technology Networks Header
Tuesday, September 16, 2014
 
Register | Sign in
Home Page > Videos > Single Copy qPCR-Based Detection of BRAF and KRAS Mutations
  Videos

Return

Single Copy qPCR-Based Detection of BRAF and KRAS Mutations
Swift Biosciences Inc

Vladimir Makarov, Chief Scientific Officer, Swift Biosciences Inc

Abstract
The development of highly sensitive genotyping assays that are suitable for clinical diagnostics opens new opportunities for the detection, assessment, and management of cancer.  Anticipated uses for these assays include profiling tissue biopsies, circulating tumor cells (CTCs), and detecting mutations in circulating cell-free nucleic acids.  Swift Biosciences™ has developed myT™ Primers which have unique structural and thermodynamic properties that make them highly sensitive to mismatch discrimination. A myT Primer assay for BRAF V600E/K mutations demonstrated single mutant copy sensitivity in a background of 14,000 wild-type genomic DNA copies. This assay also demonstrated high specificity as indicated by a low percentage (< 3%) of amplification events from 14,000 wild-type genomic copies. myT Primer assays are compatible with multiple qPCR instruments and reaction mixes.  myT Primer qPCR assays for seven common KRAS mutations demonstrated similar sensitivity and specificity. When compared to a leading commercially available KRAS mutation qPCR test kit, several orders of improved specificity were observed. The extreme selectivity of myT Primer assays will be especially useful for detection of mutations present at ultra-low copy number and for genotyping difficult samples such as needle biopsies, CTCs, and serum, resulting in better detection, evaluation, and monitoring of cancer.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Scientists Discover Neurochemical Imbalance in Schizophrenia
Researchers discovers that neurons from schizophrenia patients secrete higher amounts of three neurotransmitters.
Building Replacement Kidneys in the Lab
Milestone reached in work to build replacement kidneys in the lab.
Intestinal Bacteria Needed for Strong Flu Vaccine Responses in Mice
Study demonstrate a dependency on gut bacteria for strong immune responses to the seasonal flu and inactivated polio vaccines.
Stanford Engineers Aim to Connect the World with Ant-sized Radios
Costing just pennies to make, tiny radios-on-a-chip are designed to serve as controllers or sensors for the 'Internet of Things.'
Diabetes Researchers Find Faster Way to Create Insulin-producing Cells
A new protocol can turn stem cells into reliable, insulin-producing cells in about six weeks.
Genetic Testing for Prostate Cancer
Genetic testing can identify men at six-fold increased risk of prostate cancer.
Novel Method for Portable Detection of ‘Bath Salts’
Despite being outlawed in 2012 in the U.S., the synthetic drugs known as “bath salts” are still readily available in some retail shops, on the Internet and on the streets.
Mass Spectrometry in Your Hand
Electrospray arrays can dramatically downsize systems and costs for onsite chemical analysis and many other applications.
Continuous Flow Production of Metal-Organic Frameworks
An optimal continuous flow chemistry concept for the production of over kilogram per day of MOFs has been devised.
Zebrafish Genes Linked To Human Respiratory Diseases
Genes have been discovered which may be synonymous with the genes for developing hair-like structures in the human airway.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv