Corporate Banner
Satellite Banner
Technology Networks Header
Wednesday, July 23, 2014
 
Register | Sign in
Home Page > Videos > Results From Serum-Tumorautoantibody Profiling of Breast, Colon, Lung and Prostate Cancers Using a 16k Protein Array for Improving Minimal Invasive Diagnostics
  Videos

Return

Results From Serum-Tumorautoantibody Profiling of Breast, Colon, Lung and Prostate Cancers Using a 16k Protein Array for Improving Minimal Invasive Diagnostics
Austrian Institute of Technology GmbH

Andreas Weinhäusel, Senior Scientist, Austrian Institute of Technology GmbH

Abstract
In Europe there were an estimated 3.2 million new cases of cancer in 2008. The most commonly reported cases in Europe were colorectal cancers ( 13.6%), breast cancer (13.1%), lung cancer (12.2%) and prostate cancer (11.9%). These 4 big cancer entities make up more than 50% of all cancers. It is well accepted that early detection of cancer does improve patient survival, thus there is a pressing need to improve early cancer detection.  This can be best fulfilled by an easy and simple test suitable for minimal invasive testing. Tumour-associated antigens (TAA) can be detected prior to clinical diagnosis and thus would be ideal biomarkers for early detection of cancer using only a few microliters of a patient's serum.We have developed an 16k protein-microarray using the UNIPEX human cDNA expression library. During methods optimization we studies the influence of different serum and plasma sampling procedures and finally came up with an optimized protocol using purified IgG from samples for TAA profiling. This protocol enabled definition of TAA classifier panels with very promising classification success of patients versus controls for the big 4 cancer entities. Currently we set up targeted microarrays for validation of our screening results. In addition in silico design of antigenic peptides from sero-reactive clone-sequences has also been conducted and preliminary data enabled an improved classification compared to corresponding proteins. Thus multiplexed protein and peptide based serum-analyses could further improve cancer diagnostics

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
NIST Instrument Enables High-speed Chemical Imaging of Tissues
Researchers have demonstrated a dramatically improved technique for analyzing biological cells and tissues based on characteristic molecular vibration "signatures."
Transplanting Gene into Injured Hearts Creates Biological Pacemakers
Researchers develop first minimally invasive gene therapy procedure to treat heart rhythm disorders by transforming ordinary heart muscle cells into specialized rhythm-keeping cells, potentially eliminating future need for electronic pacemakers.
Common Gene Variants Account for Most Genetic Risk for Autism
Roles of heritability, mutations, environment estimated - NIH-funded study.
Using LC-MS to Analyse Sulfonamides in Food Grade Honey
Sulfonamides are one of a number of groups of chemicals whose presence in honey is limited by international regulations.
ChIP Sequencing Kit for Next Generation Sequencing
The assay kit from Porvair Sciences extends the benefits of their proprietary Chromatrap® solid state ChIP technology.
Illumina's HiSeq X Ten Sequencing Technology for Population Studies
Next-generation sequencing technologies to be implemented at new High-Throughput Genomics Center for the MENA region.
Foodborne Pathogen Detection Speeds Up Dramatically
Next-generation sequencing techniques allow rapidly identification of strains of salmonella, quickening responses to potential outbreaks.
Bowel Cancer Breakthrough May Benefit Thousands of Patients
Researchers at Queen’s University have discovered how two genes cause bowel cancer cells to become resistant to treatments used against the disease.
Innovative Technology Enables First Crystal Structure of mGlu5 Transmembrane Domain to be Determined
Several novel drug candidates have been identified to help treat specific neuropsychiatric disorders.
NIH Scientists Identify Gene Linked to Fatal Inflammatory Disease in Children
Repurposed drugs may offer first potential therapy.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv