Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Videos>This Video
  Videos

Return

Magneto-plasmonic Nanoparticle Platform for Capture, Separation and Enumeration of Rare Cells
SELECTBIO

Detection of disseminated tumor cells or tumor biomarkers in human fluids such as blood, urine, and saliva can provide an accessible tool for cancer detection and therapy monitoring. In particular, accurate quantitation of cancer cells in the bloodstream can help to determine prognosis and monitor the effectiveness of cancer therapy. However, the challenge of detecting circulating tumor cells (CTCs) is their rare occurrence, estimated as one to few CTCs among millions of leukocytes and billions of erythrocytes. Here we address this challenging problem by developing nanoparticle probes with multiple functionalities. Integration of multiple components in a single nanostructure is a challenging task. Most of the existing approaches to synthesis of hybrid nanoparticles require cumbersome multi-step protocols and result in nanostructures with limited tunability of physical and optical properties. Here, we developed a new type of nanoparticles which consist of primary 6 nm iron oxide core-gold shell nanoparticles that form highly uniform spherical assemblies with sizes that can be varied from ca. 70 to 180 nm. The magneto-plasmonic nanoclusters exhibit strong red-NIR absorbance and superparamagnetic properties with a high magnetic moment in an external magnetic field. We conjugated the nanoclusters with monoclonal antibodies specific for tumor biomarkers of breast, colon and skin cancers and demonstrated molecular specific optical and photoacoustic (PA) imaging with high sensitivity. We carried out experiments in whole blood from a normal volunteer spiked with various amounts of cancer cells. It was shown that cell capture efficiency exhibits a linear behavior from 5 to 500 cells per 2.5 mL of the whole blood. Furthermore, we demonstrated that molecular targeted nanoclusters can be used for simultaneous magnetic capture and PA detection of cancer cells in whole blood with greater than 90% capturing efficiency with no laborious processing steps that are commonly used in other cancer cell capture and enumeration assays. The magneto-plasmonic nanoparticle platform is being tested in blood samples of cancer patients with metastatic disease with a very good capture efficiency as compared to FDA approved CellSearch system. Our immunotargeted nanoparticles can be easily adapted to a variety of biomarkers, targeting both surface receptor molecules and intracellular biomarkers of epithelialderived cancer cells. In this presentation we will explore the opportunities afforded by the hybrid magneto-plasmonic nanoparticles and PA imaging for the development of a low cost simple and nearly real-time assays for capture, separation and enumeration of rare cells.

Request more information
Company product page


Scientific News
The Rise of 3D Cell Culture and in vitro Model Systems for Drug Discovery and Toxicology
An overview of the current technology and the challenges and benefits over 2D cell culture models plus some of the latest advances relating to human health research.
Injecting New Life into Old Antibiotics
A new fully synthetic way to make a class of antibiotics called macrolides from simple building blocks is set to open up a new front in the fight against antimicrobial drug resistance.
Insight into Bacterial Resilience and Antibiotic Targets
Variant of CRISPR technology paired with computerized imaging reveals essential gene networks in bacteria.
Advancing Protein Visualization
Cryo-EM methods can determine structures of small proteins bound to potential drug candidates.
Alzheimer’s Protein Serves as Natural Antibiotic
Alzheimer's-associated amyloid plaques may be part of natural process to trap microbes, findings suggest new therapeutic strategies.
Slime Mold Reveals Clues to Immune Cells’ Directional Abilities
Study from UC San Diego identifies a protein involved in the directional ability of a slime mold.
How Do You Kill A Malaria Parasite?
Drexel University scientists have discovered an unusual mechanism for how two new antimalarial drugs operate: They give the parasite’s skin a boost in cholesterol, making it unable to traverse the narrow labyrinths of the human bloodstream. The drugs also seem to trick the parasite into reproducing prematurely.
Illuminating Hidden Gene Regulators
New super-resolution technique visualizes important role of short-lived enzyme clusters.
Supressing Intenstinal Analphylaxis in Peanut Allergy
Study from National Jewish Health shows that blockade of histamine receptors suppresses intestinal anaphylaxis in peanut allergy.
Genes That Increase Children's Risk Of Blood Infection Identified
A team led by Oxford University has identified genes that make certain children more susceptible to invasive bacterial infections by performing a large genome-wide association study in African children.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,100+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!