Satellite Banner
Technology Networks Header
Thursday, October 02, 2014
 
Register | Sign in
Home Page > Videos > Magneto-plasmonic Nanoparticle Platform for Capture, Separation and Enumeration of Rare Cells
  Videos

Return

Magneto-plasmonic Nanoparticle Platform for Capture, Separation and Enumeration of Rare Cells
SELECTBIO

Detection of disseminated tumor cells or tumor biomarkers in human fluids such as blood, urine, and saliva can provide an accessible tool for cancer detection and therapy monitoring. In particular, accurate quantitation of cancer cells in the bloodstream can help to determine prognosis and monitor the effectiveness of cancer therapy. However, the challenge of detecting circulating tumor cells (CTCs) is their rare occurrence, estimated as one to few CTCs among millions of leukocytes and billions of erythrocytes. Here we address this challenging problem by developing nanoparticle probes with multiple functionalities. Integration of multiple components in a single nanostructure is a challenging task. Most of the existing approaches to synthesis of hybrid nanoparticles require cumbersome multi-step protocols and result in nanostructures with limited tunability of physical and optical properties. Here, we developed a new type of nanoparticles which consist of primary 6 nm iron oxide core-gold shell nanoparticles that form highly uniform spherical assemblies with sizes that can be varied from ca. 70 to 180 nm. The magneto-plasmonic nanoclusters exhibit strong red-NIR absorbance and superparamagnetic properties with a high magnetic moment in an external magnetic field. We conjugated the nanoclusters with monoclonal antibodies specific for tumor biomarkers of breast, colon and skin cancers and demonstrated molecular specific optical and photoacoustic (PA) imaging with high sensitivity. We carried out experiments in whole blood from a normal volunteer spiked with various amounts of cancer cells. It was shown that cell capture efficiency exhibits a linear behavior from 5 to 500 cells per 2.5 mL of the whole blood. Furthermore, we demonstrated that molecular targeted nanoclusters can be used for simultaneous magnetic capture and PA detection of cancer cells in whole blood with greater than 90% capturing efficiency with no laborious processing steps that are commonly used in other cancer cell capture and enumeration assays. The magneto-plasmonic nanoparticle platform is being tested in blood samples of cancer patients with metastatic disease with a very good capture efficiency as compared to FDA approved CellSearch system. Our immunotargeted nanoparticles can be easily adapted to a variety of biomarkers, targeting both surface receptor molecules and intracellular biomarkers of epithelialderived cancer cells. In this presentation we will explore the opportunities afforded by the hybrid magneto-plasmonic nanoparticles and PA imaging for the development of a low cost simple and nearly real-time assays for capture, separation and enumeration of rare cells.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
NIH to Admit Patient Exposed to Ebola Virus for Observation
Ebola patients can be safely cared for at any hospital that follows CDC's infection control recommendations.
How to Make Stronger, “Greener” Cement
Analysis of material’s molecular structure leads to a new formula that could cut greenhouse-gas emissions.
Chemists Recruit Anthrax to Deliver Cancer Drugs
With some tinkering, a deadly protein becomes an efficient carrier for antibody drugs.
Biologists Find An Early Sign Of Cancer
Patients show boost in certain amino acids years before diagnosis of pancreatic cancer.
Researchers Develop New DNA Sequencing Method to Diagnose Tuberculosis
Teams aim to test the metagenomics technique on a wide range of samples.
UW-Madison Team Developing ‘Tissue Chip’ to Screen Neurological Toxins
A faster, more affordable way to screen for neural toxins.
A Novel Method for Portable Detection of Potent Drugs Known as ‘Bath Salts’
Researchers developed a low-cost, disposable and rapid platform for detecting bath salts.
Protein ‘Map’ Could Lead to Potent New Cancer Drugs
Findings will help scientists to design drugs that could target NMT enzyme.
Research Shows Alcohol Sensations Influenced by Genes
The research was the first to consider whether variation in the burn receptor gene might influence alcohol sensations.
Monkey Antibodies Help Fight Virus
Details of monkey antibodies against chikungunya virus could help to fight the disease in humans.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv