Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Videos>This Video
  Videos

Return

Magneto-plasmonic Nanoparticle Platform for Capture, Separation and Enumeration of Rare Cells
SELECTBIO

Detection of disseminated tumor cells or tumor biomarkers in human fluids such as blood, urine, and saliva can provide an accessible tool for cancer detection and therapy monitoring. In particular, accurate quantitation of cancer cells in the bloodstream can help to determine prognosis and monitor the effectiveness of cancer therapy. However, the challenge of detecting circulating tumor cells (CTCs) is their rare occurrence, estimated as one to few CTCs among millions of leukocytes and billions of erythrocytes. Here we address this challenging problem by developing nanoparticle probes with multiple functionalities. Integration of multiple components in a single nanostructure is a challenging task. Most of the existing approaches to synthesis of hybrid nanoparticles require cumbersome multi-step protocols and result in nanostructures with limited tunability of physical and optical properties. Here, we developed a new type of nanoparticles which consist of primary 6 nm iron oxide core-gold shell nanoparticles that form highly uniform spherical assemblies with sizes that can be varied from ca. 70 to 180 nm. The magneto-plasmonic nanoclusters exhibit strong red-NIR absorbance and superparamagnetic properties with a high magnetic moment in an external magnetic field. We conjugated the nanoclusters with monoclonal antibodies specific for tumor biomarkers of breast, colon and skin cancers and demonstrated molecular specific optical and photoacoustic (PA) imaging with high sensitivity. We carried out experiments in whole blood from a normal volunteer spiked with various amounts of cancer cells. It was shown that cell capture efficiency exhibits a linear behavior from 5 to 500 cells per 2.5 mL of the whole blood. Furthermore, we demonstrated that molecular targeted nanoclusters can be used for simultaneous magnetic capture and PA detection of cancer cells in whole blood with greater than 90% capturing efficiency with no laborious processing steps that are commonly used in other cancer cell capture and enumeration assays. The magneto-plasmonic nanoparticle platform is being tested in blood samples of cancer patients with metastatic disease with a very good capture efficiency as compared to FDA approved CellSearch system. Our immunotargeted nanoparticles can be easily adapted to a variety of biomarkers, targeting both surface receptor molecules and intracellular biomarkers of epithelialderived cancer cells. In this presentation we will explore the opportunities afforded by the hybrid magneto-plasmonic nanoparticles and PA imaging for the development of a low cost simple and nearly real-time assays for capture, separation and enumeration of rare cells.

Request more information
Company product page


Scientific News
RNAi Screening Trends
Understand current trends and learn which application areas are expected to gain in popularity over the next few years.
The Genetic Roots of Adolescent Scoliosis
Scientists at the RIKEN Center for Integrative Medical Sciences in collaboration with Keio University in Japan have discovered a gene that is linked to susceptibility of Scoliosis.
A Gene-Sequence Swap Using CRISPR to Cure Haemophilia
For the first time chromosomal defects responsible for hemophilia have been corrected in patient-specific iPSCs using CRISPR-Cas9 nucleases
Experimental MERS Vaccine Shows Promise in Animal Studies
A two-step regimen of experimental vaccines against Middle East respiratory syndrome (MERS) prompted immune responses in mice and rhesus macaques, report National Institutes of Health scientists who designed the vaccines.
New Tool Uses 'Drug Spillover' to Match Cancer Patients with Treatments
Researchers have developed a new tool that improves the ability to match drugs to disease: the Kinase Addiction Ranker (KAR) predicts what genetics are truly driving the cancer in any population of cells and chooses the best "kinase inhibitor" to silence these dangerous genetic causes of disease.
Understanding the Molecular Origin of Epigenetic Markers
Researchers at IRB Barcelona discover the molecular mechanism that determines how epigenetic markers influence gene expression.
HIV Susceptibility Linked to Little-Understood Immune Cell Class
High levels of diversity among immune cells called natural killer cells may strongly predispose people to infection by HIV, and may be driven by prior viral exposures, according to a new study.
Diagnostic Test Developed for Enterovirus D68
researchers at Washington University School of Medicine in St. Louis have developed a diagnostic test to quickly detect enterovirus D68 (EV-D68), a respiratory virus that caused unusually severe illness in children last year.
How a Kernel Got Naked and Corn Became King
Ten thousand years ago, a golden grain got naked, brought people together and grew to become one of the top agricultural commodities on the planet.
Sweet Revenge Against Superbugs
A special type of synthetic sugar could be the latest weapon in the fight against superbugs.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!