Satellite Banner
Technology Networks Header
Tuesday, September 16, 2014
 
Register | Sign in
Home Page > Videos > In-vivo Single Cell Transcript Analyses for Systems Modelling
  Videos

Return

In-vivo Single Cell Transcript Analyses for Systems Modelling
SELECTBIO

To investigate the importance of minimal residual disease of BCR-ABL expressing cells chronic myelogenous leukemia (CML) was assessed using single cell molecular analytical approaches. CML is believed to occur as a consequence of the clonal expansion of leukemic stem cells and to be maintained by an expanding population of hematopoietic stem cells that have acquired a BCR-ABL fusion gene. Recent studies indicate that primitive CML cells are less responsive to tyrosine kinase inhibitors and are a reservoir for the emergence of tyrosine kinase resistant subclones. It also has been reported that BCR-ABL mediated cell adhesion may be involved in post-therapy residual disease of CML. The natural heterogeneity seen across both attached and unattached BCR-ABL expressing populations has been investigated. The study developed a means to utilize flow-assisted cell sorting of cell lines expressing BCR-ABL to derive individual attached and unattached cell sub-populations. Using a homogenous extraction procedure, cells individually flow sorted into microtitre plates were subjected to combinations of ABL and BCR-ABL RT qPCR, and also phosphorylated BCR-ABL protein was assessed using the proximity ligation assay and revealed the existence of lowly and highly BCR-ABL expressing cell line populations. The implications of this study will be discussed along with newly derived systems models and in-vivo sample procedures employing AFM.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Scientists Discover Neurochemical Imbalance in Schizophrenia
Researchers discovers that neurons from schizophrenia patients secrete higher amounts of three neurotransmitters.
Building Replacement Kidneys in the Lab
Milestone reached in work to build replacement kidneys in the lab.
Intestinal Bacteria Needed for Strong Flu Vaccine Responses in Mice
Study demonstrate a dependency on gut bacteria for strong immune responses to the seasonal flu and inactivated polio vaccines.
Stanford Engineers Aim to Connect the World with Ant-sized Radios
Costing just pennies to make, tiny radios-on-a-chip are designed to serve as controllers or sensors for the 'Internet of Things.'
Diabetes Researchers Find Faster Way to Create Insulin-producing Cells
A new protocol can turn stem cells into reliable, insulin-producing cells in about six weeks.
Genetic Testing for Prostate Cancer
Genetic testing can identify men at six-fold increased risk of prostate cancer.
Novel Method for Portable Detection of ‘Bath Salts’
Despite being outlawed in 2012 in the U.S., the synthetic drugs known as “bath salts” are still readily available in some retail shops, on the Internet and on the streets.
Mass Spectrometry in Your Hand
Electrospray arrays can dramatically downsize systems and costs for onsite chemical analysis and many other applications.
Continuous Flow Production of Metal-Organic Frameworks
An optimal continuous flow chemistry concept for the production of over kilogram per day of MOFs has been devised.
Zebrafish Genes Linked To Human Respiratory Diseases
Genes have been discovered which may be synonymous with the genes for developing hair-like structures in the human airway.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv