Corporate Banner
Satellite Banner
Technology Networks Header
Wednesday, January 28, 2015
 
Register | Sign in
Home Page > Videos > In-vivo Single Cell Transcript Analyses for Systems Modelling
  Videos

Return

In-vivo Single Cell Transcript Analyses for Systems Modelling
SELECTBIO

To investigate the importance of minimal residual disease of BCR-ABL expressing cells chronic myelogenous leukemia (CML) was assessed using single cell molecular analytical approaches. CML is believed to occur as a consequence of the clonal expansion of leukemic stem cells and to be maintained by an expanding population of hematopoietic stem cells that have acquired a BCR-ABL fusion gene. Recent studies indicate that primitive CML cells are less responsive to tyrosine kinase inhibitors and are a reservoir for the emergence of tyrosine kinase resistant subclones. It also has been reported that BCR-ABL mediated cell adhesion may be involved in post-therapy residual disease of CML. The natural heterogeneity seen across both attached and unattached BCR-ABL expressing populations has been investigated. The study developed a means to utilize flow-assisted cell sorting of cell lines expressing BCR-ABL to derive individual attached and unattached cell sub-populations. Using a homogenous extraction procedure, cells individually flow sorted into microtitre plates were subjected to combinations of ABL and BCR-ABL RT qPCR, and also phosphorylated BCR-ABL protein was assessed using the proximity ligation assay and revealed the existence of lowly and highly BCR-ABL expressing cell line populations. The implications of this study will be discussed along with newly derived systems models and in-vivo sample procedures employing AFM.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
NIH Launches Tool to Advance Down Syndrome Research
Web portal will help approved professionals to plan clinical studies.
Cancer-Causing Virus Blocks Human Immune Response
Epstein-Barr virus shown to outwit the human immune response using microRNAs.
Telomere Extension Turns Back Aging Clock In Cultured Cells
Researchers delivered a modified RNA that encodes a telomere-extending protein to cultured human cells. Cell proliferation capacity was dramatically increased, yielding large numbers of cells for study.
Role of Immune System in Promoting Digestive Health
Study shows that the immune system helps to promotes digestive health by fostering community of “good” gut bacteria.
Nanodiamonds Deliver Cancer Drug To Kill Chemoresistant Cancer Stem Cells More Effectively
Delivery of Epirubicin by nanodiamonds resulted in a normally lethal dosage of Epirubicin becoming a safe and effective dosage for treatment of liver cancer.
Metabolic Mystery Solved, Lending Insight Into Lafora Disease
Scientists at the University of Kentucky College of Medicine have determined how an enzyme essential for energy metabolism functions, solving a mystery eluding molecular biologists for decades.
New Discoveries In Primary Pathway for Neurological Diseases
Discovery could lead to new drug design for neurodegenerative diseases.
Gene Changes That Affect Brain Size Identified
The identity of eight common mutations may one day reveal more about Alzheimer’s, autism and other neurological disorders.
New Revolutionary Culturing Technique for Liver and Pancreas
Culturing of mini-guts from single mouse intestine stem cells.
Noisy Data Facilitates Investigation of Breast Cancer Gene Expression
Study tested the potential of denoising autoencoders to identify and extract complex patterns from genomic data.
Scroll Up
Scroll Down
Skyscraper Banner
SELECTBIO Market Reports
LabTube - Videos for the Scientific Community
eposters - The Online Journal of Scientific Posters
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn