Corporate Banner
Satellite Banner
Technology Networks Header
Wednesday, November 26, 2014
 
Register | Sign in
Home Page > Videos > In-vivo Single Cell Transcript Analyses for Systems Modelling
  Videos

Return

In-vivo Single Cell Transcript Analyses for Systems Modelling
SELECTBIO

To investigate the importance of minimal residual disease of BCR-ABL expressing cells chronic myelogenous leukemia (CML) was assessed using single cell molecular analytical approaches. CML is believed to occur as a consequence of the clonal expansion of leukemic stem cells and to be maintained by an expanding population of hematopoietic stem cells that have acquired a BCR-ABL fusion gene. Recent studies indicate that primitive CML cells are less responsive to tyrosine kinase inhibitors and are a reservoir for the emergence of tyrosine kinase resistant subclones. It also has been reported that BCR-ABL mediated cell adhesion may be involved in post-therapy residual disease of CML. The natural heterogeneity seen across both attached and unattached BCR-ABL expressing populations has been investigated. The study developed a means to utilize flow-assisted cell sorting of cell lines expressing BCR-ABL to derive individual attached and unattached cell sub-populations. Using a homogenous extraction procedure, cells individually flow sorted into microtitre plates were subjected to combinations of ABL and BCR-ABL RT qPCR, and also phosphorylated BCR-ABL protein was assessed using the proximity ligation assay and revealed the existence of lowly and highly BCR-ABL expressing cell line populations. The implications of this study will be discussed along with newly derived systems models and in-vivo sample procedures employing AFM.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
New Advance in Cryopreservation Could Change Management of World Blood Supplies
Engineers have identified a method to rapidly prepare frozen red blood cells for transfusions.
New Study Reveals Why Some People May be Immune to HIV-1
Natural genetic variation in a protective antiviral enzyme holds promise for new therapies.
Protein Key To The Development Of Blood Stem Cells Identified
Understanding the self-replication mechanisms is critical for improving stem cell therapies for blood-related diseases and cancers.
Fast, Low-cost DNA Sequencing Technology One Step Closer To Reality
ASU Regents' Professor Stuart Lindsay led a team of scientists from Arizona State University's Biodesign Institute and IBM's T.J. Watson Research Center in the development of a prototype DNA reader that could make whole genome profiling an everyday practice in medicine.
Amazing Feet Of Science: Researchers Sequence The Centipede Genome
What it lacks in genes, it certainly makes up for in legs: the genome of the humble centipede has been found to have around 15,000 genes – around 7,000 fewer than a human.
New Genetic Markers To Combat Yellow Rust Disease In Wheat Identified
New study released identifying genetic markers that signal resistance to the wheat yellow rust pathogen.
Antibiotic Resistance Threatens Future of Modern Medicine
Overuse and misuse of antibiotics, one of the key contributors to antimicrobial resistance (AMR).
Pain In A Dish
Turning skin cells into pain-sensing neurons.
How the Environment Contributes to Human Diseases
Using a new imaging technique, NIH researchers have found that the biological machinery that builds DNA can insert molecules into the DNA strand that are damaged as a result of environmental exposures.
Monitoring Effectiveness Of Hay Fever Immunotherapy
A new test for measuring histamine release from certain white blood cells could help doctors monitor the effectiveness of immunotherapy for hay fever.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv