Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Join | Sign in
Home>Videos>This Video
  Videos

Return

3D Organotypic Liver Cultures and their Application in Predictive Toxicology
SELECTBIO

3D cultivation allows organotypic microtissue formation ensuring cell-cell contacts and contacts to the extracellular matrix. The communication between cells at tight junctions and across extracellular matrix induces and maintains cellular differentiation, functionality and viability. 3D cell cultivation techniques can tremendously improve studies on drug metabolism, drug toxicity especially long-term repeated dose effects. Using a high-throughput 3D cultivation system, we produced 3D organotypic cultures of human hepatic cells using a 96 well plate based hanging drop method. This method allows scaffold-free reorganization of cells under the force of gravity. We show that the production of 3D organotypic cultures of various hepatic cell lines and primary liver cells is possible (Mueller et al., 2011, 2013). The hepatic cells in these 3D cultures were analyzed for viability and functionality. We show that the organotypic cultures maintain high liver-specific function over 3 weeks of culture (Gunness et al., 2013). The effects of several compounds (acetaminophen, aflatoxin B, valproic acid, chlorpromazine, troglitazone and rosiglitazone) were monitored and compared. Currently we are using this system for mechanistic studies. In conclusion, better functionality of 3D systems improves the prediction of toxicity and will have impact in the changed paradigm of drug screening.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
New Molecule Could Slow The Progression Of Parkinson’s
University of Bath researchers have designed a molecule that, if developed into a drug, could slow the progression of Parkinson's Disease.
Boy or Girl? Lemur Scents Have the Answer
A new study finds that a pregnant lemur’s signature scent depends in part on whether she’s carrying a girl or a boy.
Safer Methods for Stem Cell Culturing
New study from TSRI shows that certain stem cell culture methods are associated with increased DNA mutations.
New Genomics-Driven Surveillance To Track Crop Diseases
New genomics-driven surveillance strategy to tackle emerging and re-emerging crop pathogens that threaten global food security.
Scientists Uncover New Role for Neurotransmitter that Helps Fight Infection
Scientists have shed new light on the complexities of the immune system that could help develop vaccines to boost natural defences against disease.
Key Protein That Allows Plavix To Conquer Platelets Found
The findings could lead to more personalized approaches to controlling platelet activity during heart attacks and other vascular emergencies and diseases.
World’s First Cancer Stem Cell Model From iPS Cells
Professor Masaharu Seno at the Department of Biotechnology of Okayama University, reports that cancer stem cells produced from mouse iPS cells are actually necessary for the maintenance of the cancer cells themselves.
NIH-funded Scientists Create Potential Long-acting HIV Therapeutic
New molecule also might prevent HIV infection.
Molecule Hijacks Enzyme To Boost Alcohol Metabolism
Study could lead to treatments for people with impaired acetaldehyde metabolism.
Stem Cells from Wisdom Teeth Can Be Transformed into Corneal Cells
The cells could potentially be used to treat corneal scarring.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters