Corporate Banner
Satellite Banner
Technology Networks Header
Thursday, November 27, 2014
 
Register | Sign in
Home Page > Videos > Mapping Chemical Gradients in Living Tissue in Space and Time Using Microfluidics
  Videos

Return

Mapping Chemical Gradients in Living Tissue in Space and Time Using Microfluidics
SelectBio

Chemical gradients drive many processes in biology, ranging from nerve signal transduction to ovulation. At present, microscopy is the primary tool used to understand these gradients. Microscopy has provided many important breakthroughs in our understanding of the fundamental biology, but is limited due to the need to incorporate fluorescent molecules into biological systems. As a result, there is a need to develop tools that can measure chemical gradient formation in biological systems that do not require fluorescent modification of the targets in question, can be multiplexed to measure more than one molecule and is compatible with a variety of biological sample types, including in vitro cell cultures and ex vivo tissue slices. Work from our group on the development of microfluidic tools to measure chemical gradients in living tissue will be presented. Two separate systems are under development. The first is a microfluidic system designed to analyze metabolite and protein expression from tissue. The sampling system can resolve up to 19 different ports and can be interface with either electrochemical or fluorescence-based detection methods. Using these two detection methods, we are capable of analyzing the release of either small molecule metabolites or proteins and peptides using immunoassays. The second system uses a high-density electrode array to image release of electrochemically active metabolites like nitric oxide from live tissue slices. Electrochemical characterization of this system combined with a microfluidic system for gradient generation will be shown.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Researchers Develop Efficient Method to Produce Nanoporous Metals
New technique to manufacture nanoporous metals is cheap and can be done over many scales.
Turkeys may be Lifesavers
Antibiotic to target staph infections, strep, comes from good bacteria in turkeys.
A Hybrid Vehicle That Delivers DNA
University at Buffalo researchers are developing new technology to improve DNA vaccines. The new transport system for DNA vaccines could help treat HIV, malaria, HPV and other major illnesses.
Big Data Set To Make A Big Difference In Childhood Cancer Treatment
UTS researchers are working with the Kids Research Institute to visualise large quantities of patient data to better diagnose and treat childhood cancer patients.
Two Studies Identify A Detectable, Pre-Cancerous State In The Blood
Findings pave way for new lines of cancer research focused on detection and prevention.
A Link between DNA Transcription and Disease-Causing Expansions Which Lead to Hereditary Disorders
A Tufts University study explores the relationship between transcription and the expansions of DNA repeats.
Did You Hear The One About The Two Businessmen On A Plane?
EquipNet’s vision ‘to revolutionize the way companies manage their surplus assets’ was first conceived at 30,000 feet somewhere between Chicago and Boston in 1999. Here we learn more about the company's first 15 years.
New Advance in Cryopreservation Could Change Management of World Blood Supplies
Engineers have identified a method to rapidly prepare frozen red blood cells for transfusions.
New Study Reveals Why Some People May be Immune to HIV-1
Natural genetic variation in a protective antiviral enzyme holds promise for new therapies.
Protein Key To The Development Of Blood Stem Cells Identified
Understanding the self-replication mechanisms is critical for improving stem cell therapies for blood-related diseases and cancers.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv