3D High-Content Analysis of Luminal Spaces Inside Cysts or Organoids
In this technical note, we describe how to image and analyze epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS™ high-content analysis system, overcoming the most critical of challenges of 3D assays.
You will learn how to:
-Reduce the loss of image resolution as you image deeper into the sample
-Increase throughput for 3D imaging through shorter exposure times
-Enable 3D segmentation and analysis for your high-content assays
-Decrease the number of data transfer steps between software packages