Achieve Greater Reliability in Western Blot Loading Controls
A western blot experiment generates at least two important pieces of data: a target protein blot image and a loading control blot image. Reliable assessment of changes in target protein levels by western blot requires the measurement of both target and loading control proteins in the linear dynamic range.
Unfortunately, it is not uncommon to see over-saturated band signals when using β-actin, β-tubulin or GAPDH proteins as loading controls.
Download this app note to discover:
- Why these oversaturated bands occur
- How total protein measurement can alleviate this dilemma
- A stain-free technology for visualizing and quantitating proteins in gels and blots