Automated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS
Application Note Oct 01, 2015
Beckman Coulter Life Sciences
Automated sample preparation enables highly reproducible quantitative analyses while meeting the increased efficiency needs often required for sample processing in laboratories. This application note describes results obtained using a liquid handling-based automation system for the sample preparation of compounds to be analyzed by LC-MS/MS and applied to the monitoring of multiple classes of pharmaceutical and illicit drugs. This screen panel contains 56 compounds that could be identified and quantified over a wide range of concentrations from 0.5X to 10X the cut-off value. In addition, the large majority of the compounds could be analyzed with accuracy over 80% and CV under 15%. This method provides automated capabilities for analytical laboratories that currently routinely process samples manually for the monitoring of these drugs in biological fluids.